Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20605 and HSERO_RS20610 are separated by 118 nucleotides HSERO_RS20610 and HSERO_RS20615 are separated by 86 nucleotides HSERO_RS20615 and HSERO_RS20620 are separated by 13 nucleotides
HSERO_RS20605: HSERO_RS20605 - phenylacetic acid degradation protein PaaY, at 4,726,541 to 4,727,143
_RS20605
HSERO_RS20610: HSERO_RS20610 - phenylacetate-CoA oxygenase subunit PaaA, at 4,727,262 to 4,728,248
_RS20610
HSERO_RS20615: HSERO_RS20615 - phenylacetate-CoA oxygenase subunit PaaB, at 4,728,335 to 4,728,619
_RS20615
HSERO_RS20620: HSERO_RS20620 - phenylacetic acid degradation protein, at 4,728,633 to 4,729,385
_RS20620
Position (kb) 4727
4728
4729 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4726.311 kb on + strand at 4726.326 kb on - strand at 4726.333 kb on + strand at 4726.333 kb on + strand at 4726.341 kb on - strand at 4726.362 kb on - strand at 4726.460 kb on + strand at 4726.594 kb on + strand at 4726.753 kb on - strand, within HSERO_RS20605 at 4726.753 kb on - strand, within HSERO_RS20605 at 4726.798 kb on - strand, within HSERO_RS20605 at 4727.028 kb on - strand, within HSERO_RS20605 at 4727.242 kb on - strand at 4727.298 kb on - strand at 4727.299 kb on - strand at 4727.660 kb on - strand, within HSERO_RS20610 at 4728.076 kb on + strand, within HSERO_RS20610 at 4728.400 kb on + strand, within HSERO_RS20615 at 4728.516 kb on + strand, within HSERO_RS20615 at 4728.749 kb on - strand, within HSERO_RS20620 at 4728.880 kb on + strand, within HSERO_RS20620
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 4,726,311 + +0.1 4,726,326 - -0.0 4,726,333 + -0.6 4,726,333 + +0.0 4,726,341 - +0.0 4,726,362 - -1.2 4,726,460 + -2.3 4,726,594 + -0.7 4,726,753 - HSERO_RS20605 0.35 +0.5 4,726,753 - HSERO_RS20605 0.35 -1.3 4,726,798 - HSERO_RS20605 0.43 -1.2 4,727,028 - HSERO_RS20605 0.81 +2.0 4,727,242 - -0.4 4,727,298 - +0.0 4,727,299 - +0.7 4,727,660 - HSERO_RS20610 0.40 +0.9 4,728,076 + HSERO_RS20610 0.82 +0.1 4,728,400 + HSERO_RS20615 0.23 -0.1 4,728,516 + HSERO_RS20615 0.64 +0.0 4,728,749 - HSERO_RS20620 0.15 +1.0 4,728,880 + HSERO_RS20620 0.33 -0.2
Or see this region's nucleotide sequence