Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS19725 and HSERO_RS19730 are separated by 40 nucleotides HSERO_RS19730 and HSERO_RS19735 are separated by 49 nucleotides HSERO_RS19735 and HSERO_RS19740 are separated by 362 nucleotides
HSERO_RS19725: HSERO_RS19725 - glycosyl hydrolase, at 4,524,725 to 4,525,762
_RS19725
HSERO_RS19730: HSERO_RS19730 - short-chain dehydrogenase, at 4,525,803 to 4,526,597
_RS19730
HSERO_RS19735: HSERO_RS19735 - acetolactate synthase, at 4,526,647 to 4,528,203
_RS19735
HSERO_RS19740: HSERO_RS19740 - transcriptional regulator, at 4,528,566 to 4,529,300
_RS19740
Position (kb)
4526
4527
4528
4529 Strain fitness (log2 ratio)
-1
0
1 at 4525.688 kb on + strand at 4525.713 kb on - strand at 4525.732 kb on - strand at 4525.884 kb on + strand at 4525.940 kb on + strand, within HSERO_RS19730 at 4525.953 kb on - strand, within HSERO_RS19730 at 4525.964 kb on + strand, within HSERO_RS19730 at 4525.964 kb on + strand, within HSERO_RS19730 at 4525.964 kb on + strand, within HSERO_RS19730 at 4526.077 kb on - strand, within HSERO_RS19730 at 4526.097 kb on - strand, within HSERO_RS19730 at 4526.132 kb on + strand, within HSERO_RS19730 at 4526.248 kb on - strand, within HSERO_RS19730 at 4526.461 kb on + strand, within HSERO_RS19730 at 4526.475 kb on - strand, within HSERO_RS19730 at 4526.580 kb on - strand at 4526.628 kb on - strand at 4526.731 kb on - strand at 4526.747 kb on + strand at 4526.747 kb on + strand at 4526.747 kb on + strand at 4526.747 kb on + strand at 4526.747 kb on + strand at 4526.755 kb on - strand at 4526.926 kb on + strand, within HSERO_RS19735 at 4527.216 kb on - strand, within HSERO_RS19735 at 4527.263 kb on - strand, within HSERO_RS19735 at 4527.424 kb on - strand, within HSERO_RS19735 at 4527.521 kb on - strand, within HSERO_RS19735 at 4527.695 kb on - strand, within HSERO_RS19735 at 4528.121 kb on + strand at 4528.149 kb on - strand at 4528.187 kb on + strand at 4528.254 kb on + strand at 4528.262 kb on - strand at 4528.542 kb on - strand at 4528.556 kb on + strand at 4528.564 kb on - strand at 4528.564 kb on - strand at 4528.564 kb on - strand at 4528.656 kb on + strand, within HSERO_RS19740 at 4528.752 kb on - strand, within HSERO_RS19740 at 4528.793 kb on + strand, within HSERO_RS19740 at 4528.917 kb on + strand, within HSERO_RS19740 at 4529.108 kb on - strand, within HSERO_RS19740 at 4529.186 kb on + strand, within HSERO_RS19740 at 4529.189 kb on - strand, within HSERO_RS19740 at 4529.191 kb on + strand, within HSERO_RS19740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 4,525,688 + -0.8 4,525,713 - -0.6 4,525,732 - -0.1 4,525,884 + -0.3 4,525,940 + HSERO_RS19730 0.17 -0.6 4,525,953 - HSERO_RS19730 0.19 +1.3 4,525,964 + HSERO_RS19730 0.20 -1.5 4,525,964 + HSERO_RS19730 0.20 -0.6 4,525,964 + HSERO_RS19730 0.20 +0.2 4,526,077 - HSERO_RS19730 0.34 +1.0 4,526,097 - HSERO_RS19730 0.37 +1.3 4,526,132 + HSERO_RS19730 0.41 -0.1 4,526,248 - HSERO_RS19730 0.56 -1.2 4,526,461 + HSERO_RS19730 0.83 -1.0 4,526,475 - HSERO_RS19730 0.85 +0.6 4,526,580 - +0.4 4,526,628 - +1.2 4,526,731 - +0.2 4,526,747 + +0.4 4,526,747 + -0.4 4,526,747 + -0.1 4,526,747 + +0.0 4,526,747 + -0.1 4,526,755 - -0.4 4,526,926 + HSERO_RS19735 0.18 +1.5 4,527,216 - HSERO_RS19735 0.37 -0.1 4,527,263 - HSERO_RS19735 0.40 +1.3 4,527,424 - HSERO_RS19735 0.50 -0.1 4,527,521 - HSERO_RS19735 0.56 +0.3 4,527,695 - HSERO_RS19735 0.67 -0.0 4,528,121 + +0.1 4,528,149 - +0.3 4,528,187 + -0.5 4,528,254 + +0.3 4,528,262 - -0.5 4,528,542 - +0.2 4,528,556 + -0.0 4,528,564 - +0.4 4,528,564 - -1.0 4,528,564 - +1.1 4,528,656 + HSERO_RS19740 0.12 +0.7 4,528,752 - HSERO_RS19740 0.25 +0.8 4,528,793 + HSERO_RS19740 0.31 +0.3 4,528,917 + HSERO_RS19740 0.48 -0.4 4,529,108 - HSERO_RS19740 0.74 -0.1 4,529,186 + HSERO_RS19740 0.84 +0.1 4,529,189 - HSERO_RS19740 0.85 +0.5 4,529,191 + HSERO_RS19740 0.85 -0.5
Or see this region's nucleotide sequence