Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS19145 and HSERO_RS19150 are separated by 50 nucleotides HSERO_RS19150 and HSERO_RS19155 are separated by 16 nucleotides HSERO_RS19155 and HSERO_RS19160 are separated by 274 nucleotides
HSERO_RS19145: HSERO_RS19145 - drug/metabolite transporter (DMT) superfamily permease, at 4,392,992 to 4,393,909
_RS19145
HSERO_RS19150: HSERO_RS19150 - 3-mercaptopyruvate sulfurtransferase, at 4,393,960 to 4,394,817
_RS19150
HSERO_RS19155: HSERO_RS19155 - LysR family transcriptional regulator, at 4,394,834 to 4,395,757
_RS19155
HSERO_RS19160: HSERO_RS19160 - divalent cation transporter, at 4,396,032 to 4,396,742
_RS19160
Position (kb) 4394
4395
4396 Strain fitness (log2 ratio) -1
0
1 at 4393.844 kb on - strand at 4393.876 kb on + strand at 4393.883 kb on + strand at 4393.904 kb on + strand at 4393.911 kb on - strand at 4393.912 kb on - strand at 4393.926 kb on - strand at 4394.474 kb on - strand, within HSERO_RS19150 at 4394.474 kb on - strand, within HSERO_RS19150 at 4394.476 kb on - strand, within HSERO_RS19150 at 4394.610 kb on + strand, within HSERO_RS19150 at 4394.917 kb on + strand at 4395.241 kb on - strand, within HSERO_RS19155 at 4395.509 kb on + strand, within HSERO_RS19155 at 4395.561 kb on + strand, within HSERO_RS19155 at 4395.641 kb on - strand, within HSERO_RS19155 at 4395.793 kb on - strand at 4395.850 kb on + strand at 4395.882 kb on - strand at 4395.979 kb on - strand at 4396.184 kb on - strand, within HSERO_RS19160 at 4396.391 kb on + strand, within HSERO_RS19160 at 4396.629 kb on - strand, within HSERO_RS19160 at 4396.642 kb on + strand, within HSERO_RS19160 at 4396.649 kb on - strand, within HSERO_RS19160
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 4,393,844 - +0.4 4,393,876 + -0.3 4,393,883 + -0.5 4,393,904 + -0.2 4,393,911 - +0.5 4,393,912 - +0.0 4,393,926 - -0.6 4,394,474 - HSERO_RS19150 0.60 -0.2 4,394,474 - HSERO_RS19150 0.60 +0.3 4,394,476 - HSERO_RS19150 0.60 +0.2 4,394,610 + HSERO_RS19150 0.76 +0.1 4,394,917 + +0.7 4,395,241 - HSERO_RS19155 0.44 +0.7 4,395,509 + HSERO_RS19155 0.73 +0.5 4,395,561 + HSERO_RS19155 0.79 +0.2 4,395,641 - HSERO_RS19155 0.87 +0.6 4,395,793 - -0.5 4,395,850 + +0.0 4,395,882 - -0.6 4,395,979 - -0.1 4,396,184 - HSERO_RS19160 0.21 -0.4 4,396,391 + HSERO_RS19160 0.50 -0.3 4,396,629 - HSERO_RS19160 0.84 -0.5 4,396,642 + HSERO_RS19160 0.86 -0.1 4,396,649 - HSERO_RS19160 0.87 -1.2
Or see this region's nucleotide sequence