Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS18520 and HSERO_RS18525 overlap by 4 nucleotides HSERO_RS18525 and HSERO_RS18530 are separated by 13 nucleotides
HSERO_RS18520: HSERO_RS18520 - exporter, at 4,249,007 to 4,251,439
_RS18520
HSERO_RS18525: HSERO_RS18525 - hypothetical protein, at 4,251,436 to 4,252,005
_RS18525
HSERO_RS18530: HSERO_RS18530 - 3-oxoacyl-ACP synthase, at 4,252,019 to 4,253,218
_RS18530
Position (kb)
4251
4252
4253 Strain fitness (log2 ratio)
-1
0
1
2 at 4250.466 kb on + strand, within HSERO_RS18520 at 4250.474 kb on - strand, within HSERO_RS18520 at 4250.484 kb on - strand, within HSERO_RS18520 at 4250.496 kb on + strand, within HSERO_RS18520 at 4250.598 kb on - strand, within HSERO_RS18520 at 4250.604 kb on + strand, within HSERO_RS18520 at 4250.636 kb on - strand at 4250.682 kb on + strand, within HSERO_RS18520 at 4250.760 kb on - strand, within HSERO_RS18520 at 4250.805 kb on - strand, within HSERO_RS18520 at 4250.966 kb on + strand, within HSERO_RS18520 at 4251.174 kb on + strand, within HSERO_RS18520 at 4251.311 kb on - strand at 4251.563 kb on + strand, within HSERO_RS18525 at 4251.755 kb on + strand, within HSERO_RS18525 at 4251.763 kb on - strand, within HSERO_RS18525 at 4251.864 kb on - strand, within HSERO_RS18525 at 4251.972 kb on + strand at 4251.988 kb on + strand at 4251.991 kb on - strand at 4252.031 kb on - strand at 4252.165 kb on - strand, within HSERO_RS18530 at 4252.268 kb on - strand, within HSERO_RS18530 at 4252.469 kb on + strand, within HSERO_RS18530 at 4252.477 kb on - strand, within HSERO_RS18530 at 4252.477 kb on - strand, within HSERO_RS18530 at 4252.542 kb on + strand, within HSERO_RS18530 at 4252.550 kb on - strand, within HSERO_RS18530 at 4252.622 kb on + strand, within HSERO_RS18530 at 4252.707 kb on - strand, within HSERO_RS18530 at 4252.962 kb on - strand, within HSERO_RS18530 at 4252.971 kb on + strand, within HSERO_RS18530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 4,250,466 + HSERO_RS18520 0.60 +1.9 4,250,474 - HSERO_RS18520 0.60 +0.5 4,250,484 - HSERO_RS18520 0.61 +0.4 4,250,496 + HSERO_RS18520 0.61 +0.6 4,250,598 - HSERO_RS18520 0.65 +0.5 4,250,604 + HSERO_RS18520 0.66 -0.9 4,250,636 - -0.3 4,250,682 + HSERO_RS18520 0.69 +1.5 4,250,760 - HSERO_RS18520 0.72 -1.1 4,250,805 - HSERO_RS18520 0.74 +0.2 4,250,966 + HSERO_RS18520 0.81 +2.1 4,251,174 + HSERO_RS18520 0.89 +0.5 4,251,311 - -0.2 4,251,563 + HSERO_RS18525 0.22 +0.5 4,251,755 + HSERO_RS18525 0.56 +0.2 4,251,763 - HSERO_RS18525 0.57 +0.2 4,251,864 - HSERO_RS18525 0.75 +0.7 4,251,972 + -0.7 4,251,988 + +0.0 4,251,991 - +0.4 4,252,031 - +0.4 4,252,165 - HSERO_RS18530 0.12 -0.2 4,252,268 - HSERO_RS18530 0.21 +0.0 4,252,469 + HSERO_RS18530 0.38 +0.1 4,252,477 - HSERO_RS18530 0.38 -0.0 4,252,477 - HSERO_RS18530 0.38 +0.1 4,252,542 + HSERO_RS18530 0.44 -1.5 4,252,550 - HSERO_RS18530 0.44 +0.3 4,252,622 + HSERO_RS18530 0.50 +0.9 4,252,707 - HSERO_RS18530 0.57 +0.3 4,252,962 - HSERO_RS18530 0.79 +0.0 4,252,971 + HSERO_RS18530 0.79 +0.3
Or see this region's nucleotide sequence