Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14145 and HSERO_RS14150 are separated by 562 nucleotides HSERO_RS14150 and HSERO_RS14155 are separated by 3 nucleotides
HSERO_RS14145: HSERO_RS14145 - ArsR family transcriptional regulator, at 3,218,624 to 3,219,088
_RS14145
HSERO_RS14150: HSERO_RS14150 - RNA polymerase sigma factor, at 3,219,651 to 3,220,121
_RS14150
HSERO_RS14155: HSERO_RS14155 - Fe2+-dicitrate sensor membrane protein, at 3,220,125 to 3,221,066
_RS14155
Position (kb)
3219
3220
3221 Strain fitness (log2 ratio)
-1
0
1 at 3218.652 kb on + strand at 3218.660 kb on + strand at 3218.694 kb on + strand, within HSERO_RS14145 at 3218.722 kb on - strand, within HSERO_RS14145 at 3218.722 kb on - strand, within HSERO_RS14145 at 3218.743 kb on + strand, within HSERO_RS14145 at 3218.758 kb on + strand, within HSERO_RS14145 at 3218.766 kb on - strand, within HSERO_RS14145 at 3218.766 kb on - strand, within HSERO_RS14145 at 3218.766 kb on - strand, within HSERO_RS14145 at 3218.802 kb on + strand, within HSERO_RS14145 at 3218.803 kb on + strand, within HSERO_RS14145 at 3218.932 kb on - strand, within HSERO_RS14145 at 3218.983 kb on + strand, within HSERO_RS14145 at 3218.983 kb on + strand, within HSERO_RS14145 at 3218.991 kb on - strand, within HSERO_RS14145 at 3219.075 kb on + strand at 3219.117 kb on + strand at 3219.120 kb on - strand at 3219.144 kb on + strand at 3219.171 kb on - strand at 3219.263 kb on + strand at 3219.264 kb on + strand at 3219.272 kb on - strand at 3219.272 kb on - strand at 3219.272 kb on - strand at 3219.272 kb on - strand at 3219.281 kb on - strand at 3219.286 kb on - strand at 3219.291 kb on - strand at 3219.334 kb on + strand at 3219.342 kb on - strand at 3219.348 kb on + strand at 3219.348 kb on + strand at 3219.360 kb on + strand at 3219.387 kb on - strand at 3219.387 kb on - strand at 3219.411 kb on - strand at 3219.565 kb on - strand at 3219.585 kb on + strand at 3219.673 kb on + strand at 3219.681 kb on - strand at 3219.690 kb on - strand at 3219.944 kb on - strand, within HSERO_RS14150 at 3220.123 kb on + strand at 3220.176 kb on + strand at 3220.346 kb on + strand, within HSERO_RS14155 at 3220.408 kb on - strand, within HSERO_RS14155 at 3220.562 kb on + strand, within HSERO_RS14155 at 3220.609 kb on + strand, within HSERO_RS14155 at 3220.652 kb on + strand, within HSERO_RS14155 at 3220.710 kb on + strand, within HSERO_RS14155 at 3220.710 kb on + strand, within HSERO_RS14155 at 3220.710 kb on + strand, within HSERO_RS14155 at 3220.718 kb on - strand, within HSERO_RS14155 at 3221.008 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 3,218,652 + -0.4 3,218,660 + -0.1 3,218,694 + HSERO_RS14145 0.15 +0.4 3,218,722 - HSERO_RS14145 0.21 -0.5 3,218,722 - HSERO_RS14145 0.21 +0.0 3,218,743 + HSERO_RS14145 0.26 -0.1 3,218,758 + HSERO_RS14145 0.29 +0.4 3,218,766 - HSERO_RS14145 0.31 +0.4 3,218,766 - HSERO_RS14145 0.31 -0.1 3,218,766 - HSERO_RS14145 0.31 +1.0 3,218,802 + HSERO_RS14145 0.38 -0.1 3,218,803 + HSERO_RS14145 0.38 +0.2 3,218,932 - HSERO_RS14145 0.66 -0.5 3,218,983 + HSERO_RS14145 0.77 +0.4 3,218,983 + HSERO_RS14145 0.77 +0.0 3,218,991 - HSERO_RS14145 0.79 -0.2 3,219,075 + +0.2 3,219,117 + -0.5 3,219,120 - +0.2 3,219,144 + +0.4 3,219,171 - +0.3 3,219,263 + -0.6 3,219,264 + -0.3 3,219,272 - -0.6 3,219,272 - +0.6 3,219,272 - -0.0 3,219,272 - +0.2 3,219,281 - +0.7 3,219,286 - +0.0 3,219,291 - -0.0 3,219,334 + -1.0 3,219,342 - -0.3 3,219,348 + -0.2 3,219,348 + +0.2 3,219,360 + +0.6 3,219,387 - +0.5 3,219,387 - -0.4 3,219,411 - -0.3 3,219,565 - +0.6 3,219,585 + +0.0 3,219,673 + -0.3 3,219,681 - +1.0 3,219,690 - +1.3 3,219,944 - HSERO_RS14150 0.62 +0.4 3,220,123 + +0.6 3,220,176 + -0.5 3,220,346 + HSERO_RS14155 0.23 +0.6 3,220,408 - HSERO_RS14155 0.30 -0.1 3,220,562 + HSERO_RS14155 0.46 -0.1 3,220,609 + HSERO_RS14155 0.51 -0.6 3,220,652 + HSERO_RS14155 0.56 -0.0 3,220,710 + HSERO_RS14155 0.62 -0.9 3,220,710 + HSERO_RS14155 0.62 -0.3 3,220,710 + HSERO_RS14155 0.62 +0.8 3,220,718 - HSERO_RS14155 0.63 +0.8 3,221,008 + -1.0
Or see this region's nucleotide sequence