Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12720 and HSERO_RS12725 are separated by 2 nucleotides HSERO_RS12725 and HSERO_RS12730 are separated by 82 nucleotides HSERO_RS12730 and HSERO_RS12735 are separated by 12 nucleotides HSERO_RS12735 and HSERO_RS12740 are separated by 44 nucleotides
HSERO_RS12720: HSERO_RS12720 - hypothetical protein, at 2,897,704 to 2,898,918
_RS12720
HSERO_RS12725: HSERO_RS12725 - GTP cyclohydrolase, at 2,898,921 to 2,899,832
_RS12725
HSERO_RS12730: HSERO_RS12730 - succinylglutamate desuccinylase, at 2,899,915 to 2,900,883
_RS12730
HSERO_RS12735: HSERO_RS12735 - GNAT family acetyltransferase, at 2,900,896 to 2,901,381
_RS12735
HSERO_RS12740: HSERO_RS12740 - histone acetyltransferase HPA2, GnaT family, acetyltransferase, at 2,901,426 to 2,901,842
_RS12740
Position (kb)
2899
2900
2901 Strain fitness (log2 ratio)
-1
0
1 at 2898.943 kb on - strand at 2898.977 kb on - strand at 2898.977 kb on - strand at 2899.082 kb on + strand, within HSERO_RS12725 at 2899.090 kb on - strand, within HSERO_RS12725 at 2899.090 kb on - strand, within HSERO_RS12725 at 2899.150 kb on + strand, within HSERO_RS12725 at 2899.415 kb on - strand, within HSERO_RS12725 at 2899.602 kb on + strand, within HSERO_RS12725 at 2899.602 kb on + strand, within HSERO_RS12725 at 2899.715 kb on + strand, within HSERO_RS12725 at 2899.750 kb on + strand at 2899.867 kb on - strand at 2899.898 kb on - strand at 2899.940 kb on - strand at 2900.139 kb on + strand, within HSERO_RS12730 at 2900.145 kb on + strand, within HSERO_RS12730 at 2900.145 kb on + strand, within HSERO_RS12730 at 2900.256 kb on + strand, within HSERO_RS12730 at 2900.318 kb on - strand, within HSERO_RS12730 at 2900.323 kb on - strand, within HSERO_RS12730 at 2900.356 kb on - strand, within HSERO_RS12730 at 2900.358 kb on + strand, within HSERO_RS12730 at 2900.366 kb on - strand, within HSERO_RS12730 at 2900.371 kb on + strand, within HSERO_RS12730 at 2900.513 kb on + strand, within HSERO_RS12730 at 2900.518 kb on + strand, within HSERO_RS12730 at 2900.912 kb on + strand at 2901.019 kb on - strand, within HSERO_RS12735 at 2901.053 kb on + strand, within HSERO_RS12735 at 2901.405 kb on - strand at 2901.628 kb on - strand, within HSERO_RS12740 at 2901.729 kb on - strand, within HSERO_RS12740 at 2901.850 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 2,898,943 - +0.1 2,898,977 - +0.4 2,898,977 - -1.2 2,899,082 + HSERO_RS12725 0.18 +0.0 2,899,090 - HSERO_RS12725 0.19 +0.0 2,899,090 - HSERO_RS12725 0.19 +0.0 2,899,150 + HSERO_RS12725 0.25 +0.5 2,899,415 - HSERO_RS12725 0.54 +0.3 2,899,602 + HSERO_RS12725 0.75 -0.8 2,899,602 + HSERO_RS12725 0.75 +1.2 2,899,715 + HSERO_RS12725 0.87 -0.8 2,899,750 + +0.1 2,899,867 - +0.2 2,899,898 - +0.4 2,899,940 - +0.1 2,900,139 + HSERO_RS12730 0.23 +0.6 2,900,145 + HSERO_RS12730 0.24 +0.3 2,900,145 + HSERO_RS12730 0.24 +0.2 2,900,256 + HSERO_RS12730 0.35 +0.8 2,900,318 - HSERO_RS12730 0.42 -0.3 2,900,323 - HSERO_RS12730 0.42 +0.4 2,900,356 - HSERO_RS12730 0.46 +0.5 2,900,358 + HSERO_RS12730 0.46 +0.4 2,900,366 - HSERO_RS12730 0.47 +0.3 2,900,371 + HSERO_RS12730 0.47 +0.3 2,900,513 + HSERO_RS12730 0.62 +0.8 2,900,518 + HSERO_RS12730 0.62 +0.6 2,900,912 + +0.1 2,901,019 - HSERO_RS12735 0.25 -0.8 2,901,053 + HSERO_RS12735 0.32 -0.2 2,901,405 - +0.6 2,901,628 - HSERO_RS12740 0.48 +0.0 2,901,729 - HSERO_RS12740 0.73 +0.0 2,901,850 + +0.5
Or see this region's nucleotide sequence