Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12140 and HSERO_RS12145 overlap by 4 nucleotides HSERO_RS12145 and HSERO_RS12150 are separated by 343 nucleotides HSERO_RS12150 and HSERO_RS12155 overlap by 1 nucleotides HSERO_RS12155 and HSERO_RS12160 are separated by 90 nucleotides
HSERO_RS12140: HSERO_RS12140 - 5-deoxyglucuronate isomerase, at 2,758,509 to 2,759,303
_RS12140
HSERO_RS12145: HSERO_RS12145 - hypothetical protein, at 2,759,300 to 2,759,617
_RS12145
HSERO_RS12150: HSERO_RS12150 - iron-regulated membrane protein, at 2,759,961 to 2,761,139
_RS12150
HSERO_RS12155: HSERO_RS12155 - hypothetical protein, at 2,761,139 to 2,761,348
_RS12155
HSERO_RS12160: HSERO_RS12160 - Fe2+-dicitrate sensor membrane protein, at 2,761,439 to 2,762,467
_RS12160
Position (kb)
2759
2760
2761
2762 Strain fitness (log2 ratio)
-1
0
1 at 2759.026 kb on - strand, within HSERO_RS12140 at 2759.075 kb on + strand, within HSERO_RS12140 at 2759.190 kb on - strand at 2759.322 kb on + strand at 2759.359 kb on - strand, within HSERO_RS12145 at 2759.403 kb on - strand, within HSERO_RS12145 at 2759.430 kb on - strand, within HSERO_RS12145 at 2759.581 kb on + strand, within HSERO_RS12145 at 2759.814 kb on - strand at 2760.116 kb on + strand, within HSERO_RS12150 at 2760.173 kb on + strand, within HSERO_RS12150 at 2760.322 kb on + strand, within HSERO_RS12150 at 2760.446 kb on + strand, within HSERO_RS12150 at 2760.865 kb on + strand, within HSERO_RS12150 at 2760.876 kb on + strand, within HSERO_RS12150 at 2760.903 kb on + strand, within HSERO_RS12150 at 2760.913 kb on + strand, within HSERO_RS12150 at 2760.991 kb on - strand, within HSERO_RS12150 at 2761.011 kb on - strand, within HSERO_RS12150 at 2761.066 kb on - strand at 2761.172 kb on - strand, within HSERO_RS12155 at 2761.296 kb on + strand, within HSERO_RS12155 at 2761.313 kb on + strand, within HSERO_RS12155 at 2761.468 kb on + strand at 2761.557 kb on + strand, within HSERO_RS12160 at 2761.565 kb on - strand, within HSERO_RS12160 at 2761.567 kb on + strand, within HSERO_RS12160 at 2761.669 kb on + strand, within HSERO_RS12160 at 2761.849 kb on + strand, within HSERO_RS12160 at 2762.101 kb on - strand, within HSERO_RS12160
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 2,759,026 - HSERO_RS12140 0.65 +0.4 2,759,075 + HSERO_RS12140 0.71 +0.4 2,759,190 - -0.5 2,759,322 + -0.3 2,759,359 - HSERO_RS12145 0.19 +0.2 2,759,403 - HSERO_RS12145 0.32 -0.6 2,759,430 - HSERO_RS12145 0.41 -0.0 2,759,581 + HSERO_RS12145 0.88 -0.2 2,759,814 - -0.3 2,760,116 + HSERO_RS12150 0.13 +1.2 2,760,173 + HSERO_RS12150 0.18 +0.4 2,760,322 + HSERO_RS12150 0.31 +0.5 2,760,446 + HSERO_RS12150 0.41 +0.3 2,760,865 + HSERO_RS12150 0.77 +0.0 2,760,876 + HSERO_RS12150 0.78 +0.2 2,760,903 + HSERO_RS12150 0.80 -0.3 2,760,913 + HSERO_RS12150 0.81 +0.5 2,760,991 - HSERO_RS12150 0.87 -0.1 2,761,011 - HSERO_RS12150 0.89 +1.4 2,761,066 - -1.3 2,761,172 - HSERO_RS12155 0.16 -0.0 2,761,296 + HSERO_RS12155 0.75 -0.7 2,761,313 + HSERO_RS12155 0.83 -0.5 2,761,468 + +0.5 2,761,557 + HSERO_RS12160 0.11 -0.3 2,761,565 - HSERO_RS12160 0.12 +0.1 2,761,567 + HSERO_RS12160 0.12 +0.5 2,761,669 + HSERO_RS12160 0.22 +0.2 2,761,849 + HSERO_RS12160 0.40 +0.0 2,762,101 - HSERO_RS12160 0.64 +0.3
Or see this region's nucleotide sequence