Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10010 and HSERO_RS10015 are separated by 124 nucleotides HSERO_RS10015 and HSERO_RS10020 overlap by 1 nucleotides HSERO_RS10020 and HSERO_RS10025 are separated by 55 nucleotides HSERO_RS10025 and HSERO_RS10030 are separated by 293 nucleotides
HSERO_RS10010: HSERO_RS10010 - UDP-glucose 6-dehydrogenase, at 2,265,440 to 2,266,816
_RS10010
HSERO_RS10015: HSERO_RS10015 - hypothetical protein, at 2,266,941 to 2,267,687
_RS10015
HSERO_RS10020: HSERO_RS10020 - flippase, at 2,267,687 to 2,269,117
_RS10020
HSERO_RS10025: HSERO_RS10025 - hypothetical protein, at 2,269,173 to 2,269,787
_RS10025
HSERO_RS10030: HSERO_RS10030 - acyltransferase, at 2,270,081 to 2,271,019
_RS10030
Position (kb)
2267
2268
2269
2270 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2267.185 kb on + strand, within HSERO_RS10015 at 2267.689 kb on + strand at 2267.697 kb on - strand at 2267.789 kb on - strand at 2267.989 kb on - strand at 2268.587 kb on - strand, within HSERO_RS10020 at 2268.626 kb on + strand, within HSERO_RS10020 at 2268.647 kb on + strand, within HSERO_RS10020 at 2270.113 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 2,267,185 + HSERO_RS10015 0.33 -0.4 2,267,689 + -0.8 2,267,697 - +0.5 2,267,789 - +0.0 2,267,989 - +0.8 2,268,587 - HSERO_RS10020 0.63 +0.3 2,268,626 + HSERO_RS10020 0.66 +0.3 2,268,647 + HSERO_RS10020 0.67 +3.0 2,270,113 + +0.6
Or see this region's nucleotide sequence