Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS08350 and HSERO_RS08355 overlap by 4 nucleotides HSERO_RS08355 and HSERO_RS08360 are separated by 28 nucleotides HSERO_RS08360 and HSERO_RS08365 are separated by 114 nucleotides
HSERO_RS08350: HSERO_RS08350 - esterase, at 1,901,946 to 1,902,824
_RS08350
HSERO_RS08355: HSERO_RS08355 - dioxygenase, at 1,902,821 to 1,903,474
_RS08355
HSERO_RS08360: HSERO_RS08360 - multidrug DMT transporter permease, at 1,903,503 to 1,904,363
_RS08360
HSERO_RS08365: HSERO_RS08365 - AraC family transcriptional regulator, at 1,904,478 to 1,905,299
_RS08365
Position (kb)
1903
1904
1905 Strain fitness (log2 ratio)
-1
0
1
2 at 1902.567 kb on + strand, within HSERO_RS08350 at 1902.567 kb on + strand, within HSERO_RS08350 at 1902.591 kb on + strand, within HSERO_RS08350 at 1902.638 kb on + strand, within HSERO_RS08350 at 1902.720 kb on + strand, within HSERO_RS08350 at 1902.757 kb on + strand at 1902.767 kb on + strand at 1903.001 kb on - strand, within HSERO_RS08355 at 1903.140 kb on + strand, within HSERO_RS08355 at 1903.345 kb on - strand, within HSERO_RS08355 at 1903.355 kb on - strand, within HSERO_RS08355 at 1903.368 kb on - strand, within HSERO_RS08355 at 1903.368 kb on - strand, within HSERO_RS08355 at 1903.584 kb on + strand at 1903.627 kb on + strand, within HSERO_RS08360 at 1903.627 kb on + strand, within HSERO_RS08360 at 1903.668 kb on - strand, within HSERO_RS08360 at 1903.729 kb on + strand, within HSERO_RS08360 at 1903.742 kb on - strand, within HSERO_RS08360 at 1903.783 kb on - strand, within HSERO_RS08360 at 1903.799 kb on + strand, within HSERO_RS08360 at 1903.834 kb on + strand, within HSERO_RS08360 at 1903.877 kb on - strand, within HSERO_RS08360 at 1903.995 kb on - strand, within HSERO_RS08360 at 1903.997 kb on + strand, within HSERO_RS08360 at 1904.142 kb on - strand, within HSERO_RS08360 at 1904.192 kb on - strand, within HSERO_RS08360 at 1904.267 kb on + strand, within HSERO_RS08360 at 1904.367 kb on + strand at 1904.406 kb on - strand at 1904.589 kb on + strand at 1904.589 kb on + strand, within HSERO_RS08365 at 1904.592 kb on - strand, within HSERO_RS08365 at 1904.592 kb on - strand, within HSERO_RS08365 at 1904.597 kb on - strand, within HSERO_RS08365 at 1904.639 kb on - strand, within HSERO_RS08365 at 1904.674 kb on + strand, within HSERO_RS08365 at 1904.872 kb on - strand, within HSERO_RS08365 at 1905.179 kb on - strand, within HSERO_RS08365 at 1905.192 kb on - strand, within HSERO_RS08365 at 1905.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 1,902,567 + HSERO_RS08350 0.71 +0.3 1,902,567 + HSERO_RS08350 0.71 +0.7 1,902,591 + HSERO_RS08350 0.73 -0.2 1,902,638 + HSERO_RS08350 0.79 -0.4 1,902,720 + HSERO_RS08350 0.88 +0.3 1,902,757 + +0.6 1,902,767 + -0.1 1,903,001 - HSERO_RS08355 0.28 +0.9 1,903,140 + HSERO_RS08355 0.49 +0.1 1,903,345 - HSERO_RS08355 0.80 -0.4 1,903,355 - HSERO_RS08355 0.82 -0.1 1,903,368 - HSERO_RS08355 0.84 -0.3 1,903,368 - HSERO_RS08355 0.84 -0.1 1,903,584 + +0.6 1,903,627 + HSERO_RS08360 0.14 +0.4 1,903,627 + HSERO_RS08360 0.14 +0.1 1,903,668 - HSERO_RS08360 0.19 +0.7 1,903,729 + HSERO_RS08360 0.26 +0.7 1,903,742 - HSERO_RS08360 0.28 -0.1 1,903,783 - HSERO_RS08360 0.33 -0.0 1,903,799 + HSERO_RS08360 0.34 +0.7 1,903,834 + HSERO_RS08360 0.38 +2.4 1,903,877 - HSERO_RS08360 0.43 -0.1 1,903,995 - HSERO_RS08360 0.57 +1.2 1,903,997 + HSERO_RS08360 0.57 +0.8 1,904,142 - HSERO_RS08360 0.74 +0.2 1,904,192 - HSERO_RS08360 0.80 +0.0 1,904,267 + HSERO_RS08360 0.89 -0.2 1,904,367 + +0.1 1,904,406 - -0.4 1,904,589 + +1.1 1,904,589 + HSERO_RS08365 0.14 -0.3 1,904,592 - HSERO_RS08365 0.14 -0.6 1,904,592 - HSERO_RS08365 0.14 -0.3 1,904,597 - HSERO_RS08365 0.14 +0.2 1,904,639 - HSERO_RS08365 0.20 +0.5 1,904,674 + HSERO_RS08365 0.24 -0.6 1,904,872 - HSERO_RS08365 0.48 -0.5 1,905,179 - HSERO_RS08365 0.85 -0.2 1,905,192 - HSERO_RS08365 0.87 -0.6 1,905,310 - +0.9
Or see this region's nucleotide sequence