Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04005 and HSERO_RS04010 are separated by 11 nucleotides HSERO_RS04010 and HSERO_RS04015 are separated by 247 nucleotides HSERO_RS04015 and HSERO_RS04020 are separated by 246 nucleotides HSERO_RS04020 and HSERO_RS04025 are separated by 4 nucleotides HSERO_RS04025 and HSERO_RS04030 overlap by 4 nucleotides
HSERO_RS04005: HSERO_RS04005 - type III secretion protein, at 877,507 to 878,358
_RS04005
HSERO_RS04010: HSERO_RS04010 - hypothetical protein, at 878,370 to 878,840
_RS04010
HSERO_RS04015: HSERO_RS04015 - flagellar biosynthesis/type III secretory pathway protein, at 879,088 to 879,624
_RS04015
HSERO_RS04020: HSERO_RS04020 - hypothetical protein, at 879,871 to 880,104
_RS04020
HSERO_RS04025: HSERO_RS04025 - hypothetical protein, at 880,109 to 880,522
_RS04025
HSERO_RS04030: HSERO_RS04030 - type II/III HrcC transmembrane protein, at 880,519 to 882,600
_RS04030
Position (kb) 879
880 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 878.193 kb on + strand at 878.193 kb on + strand, within HSERO_RS04005 at 878.283 kb on + strand at 878.291 kb on - strand at 878.303 kb on - strand at 878.303 kb on - strand at 878.358 kb on + strand at 878.371 kb on - strand at 878.473 kb on + strand, within HSERO_RS04010 at 878.542 kb on + strand, within HSERO_RS04010 at 878.604 kb on - strand, within HSERO_RS04010 at 878.682 kb on + strand, within HSERO_RS04010 at 879.011 kb on - strand at 879.011 kb on - strand at 879.011 kb on - strand at 879.016 kb on + strand at 879.106 kb on - strand at 879.251 kb on + strand, within HSERO_RS04015 at 879.453 kb on + strand, within HSERO_RS04015 at 879.557 kb on + strand, within HSERO_RS04015 at 879.595 kb on + strand at 879.867 kb on + strand at 880.082 kb on - strand at 880.324 kb on + strand, within HSERO_RS04025 at 880.404 kb on + strand, within HSERO_RS04025 at 880.507 kb on - strand at 880.599 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 878,193 + +3.2 878,193 + HSERO_RS04005 0.81 -0.1 878,283 + +0.2 878,291 - -0.6 878,303 - +0.1 878,303 - +0.5 878,358 + -0.1 878,371 - +0.5 878,473 + HSERO_RS04010 0.22 +0.2 878,542 + HSERO_RS04010 0.37 +0.2 878,604 - HSERO_RS04010 0.50 +0.4 878,682 + HSERO_RS04010 0.66 -0.6 879,011 - +0.2 879,011 - -0.1 879,011 - +0.7 879,016 + +0.2 879,106 - -0.7 879,251 + HSERO_RS04015 0.30 +0.3 879,453 + HSERO_RS04015 0.68 +1.1 879,557 + HSERO_RS04015 0.87 -1.8 879,595 + +0.1 879,867 + -0.1 880,082 - -0.9 880,324 + HSERO_RS04025 0.52 +0.3 880,404 + HSERO_RS04025 0.71 -0.0 880,507 - +0.0 880,599 - +0.6
Or see this region's nucleotide sequence