Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03970 and HSERO_RS03975 overlap by 7 nucleotides HSERO_RS03975 and HSERO_RS03980 are separated by 65 nucleotides HSERO_RS03980 and HSERO_RS03985 are separated by 3 nucleotides HSERO_RS03985 and HSERO_RS03990 are separated by 15 nucleotides
HSERO_RS03970: HSERO_RS03970 - hypothetical protein, at 872,341 to 873,540
_RS03970
HSERO_RS03975: HSERO_RS03975 - flagellar biosynthesis protein FliP, at 873,534 to 874,196
_RS03975
HSERO_RS03980: HSERO_RS03980 - type III secretion HrcS protein, at 874,262 to 874,525
_RS03980
HSERO_RS03985: HSERO_RS03985 - type III secretion protein, at 874,529 to 875,317
_RS03985
HSERO_RS03990: HSERO_RS03990 - type III secretion protein, at 875,333 to 876,448
_RS03990
Position (kb) 874
875 Strain fitness (log2 ratio) -1
0
1
2 at 873.348 kb on - strand, within HSERO_RS03970 at 873.635 kb on - strand, within HSERO_RS03975 at 873.711 kb on + strand, within HSERO_RS03975 at 873.747 kb on + strand, within HSERO_RS03975 at 873.752 kb on + strand, within HSERO_RS03975 at 873.846 kb on - strand, within HSERO_RS03975 at 874.068 kb on - strand, within HSERO_RS03975 at 874.183 kb on + strand at 874.343 kb on - strand, within HSERO_RS03980 at 874.353 kb on - strand, within HSERO_RS03980 at 874.392 kb on + strand, within HSERO_RS03980 at 874.472 kb on + strand, within HSERO_RS03980 at 874.660 kb on - strand, within HSERO_RS03985 at 874.673 kb on + strand, within HSERO_RS03985 at 874.720 kb on - strand, within HSERO_RS03985 at 874.773 kb on - strand, within HSERO_RS03985 at 874.812 kb on + strand, within HSERO_RS03985 at 875.064 kb on - strand, within HSERO_RS03985 at 875.228 kb on + strand, within HSERO_RS03985 at 875.236 kb on - strand, within HSERO_RS03985 at 875.247 kb on + strand at 875.319 kb on - strand at 875.393 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 873,348 - HSERO_RS03970 0.84 +0.2 873,635 - HSERO_RS03975 0.15 +0.1 873,711 + HSERO_RS03975 0.27 +0.8 873,747 + HSERO_RS03975 0.32 +0.1 873,752 + HSERO_RS03975 0.33 +0.4 873,846 - HSERO_RS03975 0.47 +0.3 874,068 - HSERO_RS03975 0.81 +0.8 874,183 + -0.3 874,343 - HSERO_RS03980 0.31 +0.6 874,353 - HSERO_RS03980 0.34 +0.9 874,392 + HSERO_RS03980 0.49 -0.4 874,472 + HSERO_RS03980 0.80 +0.7 874,660 - HSERO_RS03985 0.17 -1.1 874,673 + HSERO_RS03985 0.18 -1.2 874,720 - HSERO_RS03985 0.24 +0.4 874,773 - HSERO_RS03985 0.31 +0.2 874,812 + HSERO_RS03985 0.36 +1.5 875,064 - HSERO_RS03985 0.68 +0.1 875,228 + HSERO_RS03985 0.89 +0.5 875,236 - HSERO_RS03985 0.90 -1.4 875,247 + +1.7 875,319 - -0.7 875,393 + +2.0
Or see this region's nucleotide sequence