Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01985 and HSERO_RS01990 are separated by 88 nucleotides HSERO_RS01990 and HSERO_RS01995 are separated by 26 nucleotides HSERO_RS01995 and HSERO_RS02000 are separated by 220 nucleotides
HSERO_RS01985: HSERO_RS01985 - energy transducer TonB, at 435,269 to 436,150
_RS01985
HSERO_RS01990: HSERO_RS01990 - shikimate 5-dehydrogenase, at 436,239 to 437,054
_RS01990
HSERO_RS01995: HSERO_RS01995 - peptidoglycan transglycosylase, at 437,081 to 437,788
_RS01995
HSERO_RS02000: HSERO_RS02000 - magnesium transporter, at 438,009 to 438,974
_RS02000
Position (kb) 437
438 Strain fitness (log2 ratio) -1
0
1 at 436.226 kb on + strand at 436.449 kb on - strand, within HSERO_RS01990 at 436.484 kb on - strand, within HSERO_RS01990 at 436.551 kb on - strand at 436.570 kb on - strand, within HSERO_RS01990 at 436.853 kb on - strand, within HSERO_RS01990 at 436.875 kb on - strand, within HSERO_RS01990 at 437.112 kb on - strand at 437.117 kb on + strand at 437.117 kb on + strand at 437.132 kb on - strand at 437.167 kb on + strand, within HSERO_RS01995 at 437.167 kb on + strand, within HSERO_RS01995 at 437.175 kb on - strand, within HSERO_RS01995 at 437.651 kb on - strand, within HSERO_RS01995 at 437.817 kb on - strand at 438.073 kb on + strand at 438.100 kb on - strand at 438.105 kb on - strand at 438.425 kb on + strand, within HSERO_RS02000 at 438.540 kb on - strand, within HSERO_RS02000 at 438.548 kb on - strand, within HSERO_RS02000 at 438.646 kb on + strand, within HSERO_RS02000 at 438.646 kb on + strand, within HSERO_RS02000 at 438.656 kb on + strand, within HSERO_RS02000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 436,226 + +0.0 436,449 - HSERO_RS01990 0.26 -1.0 436,484 - HSERO_RS01990 0.30 -0.7 436,551 - -0.5 436,570 - HSERO_RS01990 0.41 -0.7 436,853 - HSERO_RS01990 0.75 -0.7 436,875 - HSERO_RS01990 0.78 -0.8 437,112 - +0.2 437,117 + +0.2 437,117 + +1.0 437,132 - +0.0 437,167 + HSERO_RS01995 0.12 +0.7 437,167 + HSERO_RS01995 0.12 +0.1 437,175 - HSERO_RS01995 0.13 +0.4 437,651 - HSERO_RS01995 0.81 +1.0 437,817 - -0.5 438,073 + +0.8 438,100 - +0.8 438,105 - +0.4 438,425 + HSERO_RS02000 0.43 -0.1 438,540 - HSERO_RS02000 0.55 +0.9 438,548 - HSERO_RS02000 0.56 -0.2 438,646 + HSERO_RS02000 0.66 +0.5 438,646 + HSERO_RS02000 0.66 -1.6 438,656 + HSERO_RS02000 0.67 +0.7
Or see this region's nucleotide sequence