Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01410 and HSERO_RS01415 are separated by 75 nucleotides HSERO_RS01415 and HSERO_RS01420 are separated by 93 nucleotides HSERO_RS01420 and HSERO_RS01425 are separated by 77 nucleotides HSERO_RS01425 and HSERO_RS01430 are separated by 109 nucleotides
HSERO_RS01410: HSERO_RS01410 - hypothetical protein, at 305,960 to 306,370
_RS01410
HSERO_RS01415: HSERO_RS01415 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 306,446 to 306,805
_RS01415
HSERO_RS01420: HSERO_RS01420 - serine/threonine dehydratase, at 306,899 to 307,879
_RS01420
HSERO_RS01425: HSERO_RS01425 - DNA-binding protein, at 307,957 to 308,613
_RS01425
HSERO_RS01430: HSERO_RS01430 - phospholipase, at 308,723 to 309,973
_RS01430
Position (kb) 306
307
308 Strain fitness (log2 ratio) -1
0
1
2 at 305.945 kb on - strand at 305.982 kb on + strand at 306.213 kb on + strand, within HSERO_RS01410 at 306.256 kb on - strand at 306.346 kb on + strand at 306.609 kb on + strand, within HSERO_RS01415 at 306.617 kb on - strand, within HSERO_RS01415 at 306.645 kb on - strand, within HSERO_RS01415 at 306.760 kb on - strand, within HSERO_RS01415 at 306.837 kb on + strand at 306.871 kb on - strand at 306.929 kb on - strand at 306.931 kb on + strand at 307.093 kb on + strand, within HSERO_RS01420 at 307.093 kb on + strand, within HSERO_RS01420 at 307.193 kb on + strand, within HSERO_RS01420 at 307.316 kb on + strand, within HSERO_RS01420 at 307.559 kb on - strand, within HSERO_RS01420 at 307.895 kb on - strand at 307.994 kb on + strand at 308.009 kb on + strand at 308.151 kb on - strand, within HSERO_RS01425 at 308.214 kb on + strand, within HSERO_RS01425 at 308.408 kb on + strand, within HSERO_RS01425 at 308.449 kb on - strand, within HSERO_RS01425 at 308.728 kb on - strand at 308.771 kb on - strand at 308.860 kb on + strand, within HSERO_RS01430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 305,945 - +2.4 305,982 + +1.0 306,213 + HSERO_RS01410 0.62 +1.4 306,256 - +2.1 306,346 + +0.1 306,609 + HSERO_RS01415 0.45 +0.4 306,617 - HSERO_RS01415 0.47 +0.8 306,645 - HSERO_RS01415 0.55 +0.3 306,760 - HSERO_RS01415 0.87 +0.1 306,837 + -0.3 306,871 - -0.1 306,929 - -0.3 306,931 + +0.2 307,093 + HSERO_RS01420 0.20 -0.1 307,093 + HSERO_RS01420 0.20 +0.4 307,193 + HSERO_RS01420 0.30 +0.2 307,316 + HSERO_RS01420 0.43 +0.3 307,559 - HSERO_RS01420 0.67 +1.1 307,895 - +0.6 307,994 + -0.7 308,009 + +1.6 308,151 - HSERO_RS01425 0.30 +0.4 308,214 + HSERO_RS01425 0.39 -0.2 308,408 + HSERO_RS01425 0.69 -0.1 308,449 - HSERO_RS01425 0.75 +0.4 308,728 - -0.3 308,771 - -0.1 308,860 + HSERO_RS01430 0.11 +0.5
Or see this region's nucleotide sequence