Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01015 and HSERO_RS01020 are separated by 22 nucleotides HSERO_RS01020 and HSERO_RS01025 are separated by 29 nucleotides
HSERO_RS01015: HSERO_RS01015 - hypothetical protein, at 217,462 to 218,649
_RS01015
HSERO_RS01020: HSERO_RS01020 - hypothetical protein, at 218,672 to 219,103
_RS01020
HSERO_RS01025: HSERO_RS01025 - hypothetical protein, at 219,133 to 220,320
_RS01025
Position (kb)
218
219
220 Strain fitness (log2 ratio)
-1
0
1
2 at 217.736 kb on + strand at 217.831 kb on - strand, within HSERO_RS01015 at 217.836 kb on - strand, within HSERO_RS01015 at 217.941 kb on - strand, within HSERO_RS01015 at 217.974 kb on + strand, within HSERO_RS01015 at 218.115 kb on - strand, within HSERO_RS01015 at 218.130 kb on + strand, within HSERO_RS01015 at 218.198 kb on + strand, within HSERO_RS01015 at 218.206 kb on - strand, within HSERO_RS01015 at 218.206 kb on - strand, within HSERO_RS01015 at 218.206 kb on - strand, within HSERO_RS01015 at 218.282 kb on + strand, within HSERO_RS01015 at 218.381 kb on + strand, within HSERO_RS01015 at 218.669 kb on + strand at 218.807 kb on + strand, within HSERO_RS01020 at 218.807 kb on + strand, within HSERO_RS01020 at 218.984 kb on + strand, within HSERO_RS01020 at 218.992 kb on - strand, within HSERO_RS01020 at 219.253 kb on + strand, within HSERO_RS01025 at 219.261 kb on - strand, within HSERO_RS01025 at 219.261 kb on - strand, within HSERO_RS01025 at 219.273 kb on + strand, within HSERO_RS01025 at 219.526 kb on + strand, within HSERO_RS01025 at 219.526 kb on + strand, within HSERO_RS01025 at 219.661 kb on + strand, within HSERO_RS01025 at 219.832 kb on - strand, within HSERO_RS01025 at 219.924 kb on + strand, within HSERO_RS01025 at 219.925 kb on + strand, within HSERO_RS01025 at 219.934 kb on + strand, within HSERO_RS01025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 217,736 + +0.9 217,831 - HSERO_RS01015 0.31 -0.9 217,836 - HSERO_RS01015 0.31 +2.3 217,941 - HSERO_RS01015 0.40 -0.1 217,974 + HSERO_RS01015 0.43 -0.1 218,115 - HSERO_RS01015 0.55 -0.1 218,130 + HSERO_RS01015 0.56 -0.7 218,198 + HSERO_RS01015 0.62 +0.3 218,206 - HSERO_RS01015 0.63 -0.3 218,206 - HSERO_RS01015 0.63 +0.7 218,206 - HSERO_RS01015 0.63 -0.5 218,282 + HSERO_RS01015 0.69 +0.2 218,381 + HSERO_RS01015 0.77 -0.4 218,669 + +0.2 218,807 + HSERO_RS01020 0.31 +1.2 218,807 + HSERO_RS01020 0.31 +0.2 218,984 + HSERO_RS01020 0.72 +0.4 218,992 - HSERO_RS01020 0.74 +0.8 219,253 + HSERO_RS01025 0.10 -0.9 219,261 - HSERO_RS01025 0.11 +0.0 219,261 - HSERO_RS01025 0.11 +0.9 219,273 + HSERO_RS01025 0.12 +0.1 219,526 + HSERO_RS01025 0.33 -0.6 219,526 + HSERO_RS01025 0.33 +0.1 219,661 + HSERO_RS01025 0.44 -0.2 219,832 - HSERO_RS01025 0.59 +0.9 219,924 + HSERO_RS01025 0.67 -0.7 219,925 + HSERO_RS01025 0.67 -0.1 219,934 + HSERO_RS01025 0.67 -0.3
Or see this region's nucleotide sequence