Experiment: Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25430 and BWI76_RS25435 are separated by 103 nucleotides BWI76_RS25435 and BWI76_RS25440 are separated by 50 nucleotides BWI76_RS25440 and BWI76_RS25445 are separated by 140 nucleotides BWI76_RS25445 and BWI76_RS25450 are separated by 5 nucleotides BWI76_RS25450 and BWI76_RS25455 are separated by 7 nucleotides
BWI76_RS25430: BWI76_RS25430 - membrane protein, at 5,285,409 to 5,285,672
_RS25430
BWI76_RS25435: BWI76_RS25435 - membrane protein, at 5,285,776 to 5,286,042
_RS25435
BWI76_RS25440: BWI76_RS25440 - putative barnase inhibitor, at 5,286,093 to 5,286,368
_RS25440
BWI76_RS25445: BWI76_RS25445 - p-hydroxybenzoic acid efflux pump subunit AaeB, at 5,286,509 to 5,288,476
_RS25445
BWI76_RS25450: BWI76_RS25450 - p-hydroxybenzoic acid efflux subunit AaeA, at 5,288,482 to 5,289,414
_RS25450
BWI76_RS25455: BWI76_RS25455 - protein AaeX, at 5,289,422 to 5,289,625
_RS25455
Position (kb) 5286
5287
5288
5289 Strain fitness (log2 ratio) -2
-1
0
1 at 5285.545 kb on + strand, within BWI76_RS25430 at 5285.620 kb on + strand, within BWI76_RS25430 at 5285.632 kb on + strand, within BWI76_RS25430 at 5285.637 kb on + strand, within BWI76_RS25430 at 5285.717 kb on - strand at 5285.811 kb on + strand, within BWI76_RS25435 at 5285.891 kb on - strand, within BWI76_RS25435 at 5285.908 kb on + strand, within BWI76_RS25435 at 5286.018 kb on + strand at 5286.144 kb on - strand, within BWI76_RS25440 at 5286.144 kb on - strand, within BWI76_RS25440 at 5286.166 kb on - strand, within BWI76_RS25440 at 5286.241 kb on - strand, within BWI76_RS25440 at 5286.255 kb on - strand at 5286.268 kb on - strand, within BWI76_RS25440 at 5286.290 kb on + strand, within BWI76_RS25440 at 5286.298 kb on - strand, within BWI76_RS25440 at 5286.379 kb on - strand at 5286.420 kb on + strand at 5286.457 kb on + strand at 5286.591 kb on + strand at 5286.591 kb on + strand at 5286.953 kb on - strand, within BWI76_RS25445 at 5287.103 kb on - strand, within BWI76_RS25445 at 5287.387 kb on + strand, within BWI76_RS25445 at 5287.395 kb on - strand, within BWI76_RS25445 at 5287.579 kb on + strand, within BWI76_RS25445 at 5287.646 kb on - strand, within BWI76_RS25445 at 5287.693 kb on + strand, within BWI76_RS25445 at 5287.862 kb on - strand, within BWI76_RS25445 at 5287.921 kb on - strand, within BWI76_RS25445 at 5288.159 kb on + strand, within BWI76_RS25445 at 5288.298 kb on + strand at 5288.519 kb on - strand at 5288.576 kb on - strand, within BWI76_RS25450 at 5288.596 kb on - strand, within BWI76_RS25450 at 5288.607 kb on - strand, within BWI76_RS25450 at 5288.624 kb on + strand, within BWI76_RS25450 at 5288.734 kb on - strand, within BWI76_RS25450 at 5288.804 kb on + strand, within BWI76_RS25450 at 5288.828 kb on - strand, within BWI76_RS25450 at 5289.471 kb on + strand, within BWI76_RS25455 at 5289.471 kb on + strand, within BWI76_RS25455 at 5289.471 kb on + strand, within BWI76_RS25455
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL remove 5,285,545 + BWI76_RS25430 0.52 +0.5 5,285,620 + BWI76_RS25430 0.80 +0.6 5,285,632 + BWI76_RS25430 0.84 -0.8 5,285,637 + BWI76_RS25430 0.86 +0.5 5,285,717 - +0.3 5,285,811 + BWI76_RS25435 0.13 +0.8 5,285,891 - BWI76_RS25435 0.43 -0.7 5,285,908 + BWI76_RS25435 0.49 +0.2 5,286,018 + +0.2 5,286,144 - BWI76_RS25440 0.18 -0.5 5,286,144 - BWI76_RS25440 0.18 -0.7 5,286,166 - BWI76_RS25440 0.26 +0.9 5,286,241 - BWI76_RS25440 0.54 +0.3 5,286,255 - +0.9 5,286,268 - BWI76_RS25440 0.63 -0.4 5,286,290 + BWI76_RS25440 0.71 -0.4 5,286,298 - BWI76_RS25440 0.74 -2.0 5,286,379 - -0.4 5,286,420 + -0.7 5,286,457 + -0.3 5,286,591 + +0.2 5,286,591 + +0.6 5,286,953 - BWI76_RS25445 0.23 -0.2 5,287,103 - BWI76_RS25445 0.30 -0.6 5,287,387 + BWI76_RS25445 0.45 +0.5 5,287,395 - BWI76_RS25445 0.45 +0.9 5,287,579 + BWI76_RS25445 0.54 +0.6 5,287,646 - BWI76_RS25445 0.58 +1.7 5,287,693 + BWI76_RS25445 0.60 -0.0 5,287,862 - BWI76_RS25445 0.69 +1.6 5,287,921 - BWI76_RS25445 0.72 +1.1 5,288,159 + BWI76_RS25445 0.84 +0.0 5,288,298 + +0.7 5,288,519 - +0.9 5,288,576 - BWI76_RS25450 0.10 -0.0 5,288,596 - BWI76_RS25450 0.12 +0.3 5,288,607 - BWI76_RS25450 0.13 +0.4 5,288,624 + BWI76_RS25450 0.15 +0.5 5,288,734 - BWI76_RS25450 0.27 +0.4 5,288,804 + BWI76_RS25450 0.35 +0.4 5,288,828 - BWI76_RS25450 0.37 -2.3 5,289,471 + BWI76_RS25455 0.24 +0.9 5,289,471 + BWI76_RS25455 0.24 +0.5 5,289,471 + BWI76_RS25455 0.24 +0.3
Or see this region's nucleotide sequence