Experiment: Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06395 and BWI76_RS06400 are separated by 58 nucleotides BWI76_RS06400 and BWI76_RS06405 overlap by 38 nucleotides BWI76_RS06405 and BWI76_RS06410 are separated by 142 nucleotides BWI76_RS06410 and BWI76_RS06415 are separated by 114 nucleotides
BWI76_RS06395: BWI76_RS06395 - tRNA 4-thiouridine(8) synthase ThiI, at 1,343,831 to 1,345,279
_RS06395
BWI76_RS06400: BWI76_RS06400 - oxidative-stress-resistance chaperone, at 1,345,338 to 1,345,928
_RS06400
BWI76_RS06405: BWI76_RS06405 - 2-dehydropantoate 2-reductase, at 1,345,891 to 1,346,802
_RS06405
BWI76_RS06410: BWI76_RS06410 - YajQ family cyclic di-GMP-binding protein, at 1,346,945 to 1,347,436
_RS06410
BWI76_RS06415: BWI76_RS06415 - MFS transporter, at 1,347,551 to 1,348,915
_RS06415
Position (kb)
1345
1346
1347 Strain fitness (log2 ratio)
-1
0
1 at 1345.113 kb on - strand, within BWI76_RS06395 at 1345.420 kb on - strand, within BWI76_RS06400 at 1345.780 kb on - strand at 1345.835 kb on + strand, within BWI76_RS06400 at 1346.458 kb on - strand, within BWI76_RS06405 at 1346.480 kb on - strand, within BWI76_RS06405 at 1346.537 kb on - strand, within BWI76_RS06405 at 1346.719 kb on + strand at 1346.727 kb on - strand at 1347.242 kb on - strand, within BWI76_RS06410 at 1347.646 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL remove 1,345,113 - BWI76_RS06395 0.88 -0.7 1,345,420 - BWI76_RS06400 0.14 +0.4 1,345,780 - +0.9 1,345,835 + BWI76_RS06400 0.84 -0.3 1,346,458 - BWI76_RS06405 0.62 +0.9 1,346,480 - BWI76_RS06405 0.65 -1.7 1,346,537 - BWI76_RS06405 0.71 -0.5 1,346,719 + -1.0 1,346,727 - -0.2 1,347,242 - BWI76_RS06410 0.60 -0.0 1,347,646 - -0.7
Or see this region's nucleotide sequence