Experiment: Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02985 and BWI76_RS02990 are separated by 112 nucleotides BWI76_RS02990 and BWI76_RS02995 are separated by 12 nucleotides BWI76_RS02995 and BWI76_RS03000 are separated by 36 nucleotides BWI76_RS03000 and BWI76_RS03005 are separated by 192 nucleotides BWI76_RS03005 and BWI76_RS03010 are separated by 190 nucleotides
BWI76_RS02985: BWI76_RS02985 - reactive intermediate/imine deaminase, at 613,013 to 613,399
_RS02985
BWI76_RS02990: BWI76_RS02990 - aspartate carbamoyltransferase regulatory subunit, at 613,512 to 613,973
_RS02990
BWI76_RS02995: BWI76_RS02995 - aspartate carbamoyltransferase, at 613,986 to 614,921
_RS02995
BWI76_RS03000: BWI76_RS03000 - PyrBI operon leader peptide, at 614,958 to 615,062
_RS03000
BWI76_RS03005: BWI76_RS03005 - hypothetical protein, at 615,255 to 615,707
_RS03005
BWI76_RS03010: BWI76_RS03010 - ornithine carbamoyltransferase, at 615,898 to 616,902
_RS03010
Position (kb) 613
614
615 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 613.314 kb on - strand, within BWI76_RS02985 at 613.348 kb on + strand, within BWI76_RS02985 at 613.349 kb on - strand, within BWI76_RS02985 at 613.552 kb on - strand at 613.889 kb on + strand, within BWI76_RS02990 at 613.889 kb on + strand, within BWI76_RS02990 at 613.889 kb on + strand, within BWI76_RS02990 at 613.917 kb on + strand, within BWI76_RS02990 at 613.941 kb on - strand at 614.067 kb on + strand at 614.248 kb on - strand, within BWI76_RS02995 at 614.303 kb on - strand, within BWI76_RS02995 at 614.318 kb on + strand, within BWI76_RS02995 at 614.407 kb on - strand, within BWI76_RS02995 at 614.469 kb on + strand, within BWI76_RS02995 at 614.469 kb on + strand, within BWI76_RS02995 at 614.477 kb on - strand, within BWI76_RS02995 at 614.488 kb on - strand, within BWI76_RS02995 at 614.503 kb on - strand, within BWI76_RS02995 at 614.557 kb on - strand, within BWI76_RS02995 at 614.919 kb on - strand at 615.106 kb on - strand at 615.140 kb on - strand at 615.140 kb on - strand at 615.279 kb on + strand at 615.300 kb on + strand at 615.323 kb on + strand, within BWI76_RS03005 at 615.331 kb on - strand, within BWI76_RS03005 at 615.331 kb on - strand, within BWI76_RS03005 at 615.356 kb on + strand, within BWI76_RS03005 at 615.617 kb on + strand, within BWI76_RS03005 at 615.682 kb on - strand at 615.739 kb on - strand at 615.800 kb on - strand at 615.823 kb on + strand at 615.829 kb on - strand at 615.858 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL remove 613,314 - BWI76_RS02985 0.78 +0.1 613,348 + BWI76_RS02985 0.87 -0.6 613,349 - BWI76_RS02985 0.87 +0.5 613,552 - +0.4 613,889 + BWI76_RS02990 0.82 -0.1 613,889 + BWI76_RS02990 0.82 +0.3 613,889 + BWI76_RS02990 0.82 +0.7 613,917 + BWI76_RS02990 0.88 -0.1 613,941 - -0.4 614,067 + -4.2 614,248 - BWI76_RS02995 0.28 -4.0 614,303 - BWI76_RS02995 0.34 -2.9 614,318 + BWI76_RS02995 0.35 -3.3 614,407 - BWI76_RS02995 0.45 -5.6 614,469 + BWI76_RS02995 0.52 -3.9 614,469 + BWI76_RS02995 0.52 -2.3 614,477 - BWI76_RS02995 0.52 -5.1 614,488 - BWI76_RS02995 0.54 -4.3 614,503 - BWI76_RS02995 0.55 +1.0 614,557 - BWI76_RS02995 0.61 -3.4 614,919 - -2.3 615,106 - -0.6 615,140 - -1.4 615,140 - +0.8 615,279 + +0.8 615,300 + +0.6 615,323 + BWI76_RS03005 0.15 +0.6 615,331 - BWI76_RS03005 0.17 -1.7 615,331 - BWI76_RS03005 0.17 +0.8 615,356 + BWI76_RS03005 0.22 +0.5 615,617 + BWI76_RS03005 0.80 -2.3 615,682 - +0.2 615,739 - +0.5 615,800 - -0.5 615,823 + +1.1 615,829 - -0.3 615,858 + +0.2
Or see this region's nucleotide sequence