Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00160

Experiment: Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00155 and BWI76_RS00160 are separated by 135 nucleotidesBWI76_RS00160 and BWI76_RS00165 are separated by 316 nucleotides BWI76_RS00155: BWI76_RS00155 - phosphate transporter permease subunit PstC, at 29,676 to 30,635 _RS00155 BWI76_RS00160: BWI76_RS00160 - phosphate ABC transporter substrate-binding protein, at 30,771 to 31,811 _RS00160 BWI76_RS00165: BWI76_RS00165 - glutamine--fructose-6-phosphate aminotransferase, at 32,128 to 33,957 _RS00165 Position (kb) 30 31 32Strain fitness (log2 ratio) -2 -1 0 1at 29.795 kb on - strand, within BWI76_RS00155at 30.682 kb on - strandat 30.805 kb on - strandat 31.154 kb on - strand, within BWI76_RS00160at 31.857 kb on - strandat 31.932 kb on - strandat 32.025 kb on - strandat 32.057 kb on + strandat 32.100 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL
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29,795 - BWI76_RS00155 0.12 +0.0
30,682 - +1.0
30,805 - -0.5
31,154 - BWI76_RS00160 0.37 -1.7
31,857 - +0.0
31,932 - +1.0
32,025 - -0.2
32,057 + -2.0
32,100 + +0.0

Or see this region's nucleotide sequence