Strain Fitness in Shewanella oneidensis MR-1 around SO1824

Experiment: Ferric citrate electron acceptor; DNA cleaned with chelator

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO1822 and SO1824 are separated by 627 nucleotidesSO1824 and SO1825 overlap by 1 nucleotides SO1822: SO1822 - TonB-dependent receptor, putative (NCBI ptt file), at 1,914,178 to 1,916,988 SO1822 SO1824: SO1824 - conserved hypothetical protein (NCBI ptt file), at 1,917,616 to 1,918,281 SO1824 SO1825: SO1825 - MotA/TolQ/ExbB proton channel family protein (NCBI ptt file), at 1,918,281 to 1,919,636 SO1825 Position (kb) 1917 1918 1919Strain fitness (log2 ratio) -1 0 1at 1916.625 kb on + strand, within SO1822at 1916.633 kb on - strand, within SO1822at 1916.660 kb on - strand, within SO1822at 1916.796 kb on - strandat 1916.864 kb on + strandat 1916.924 kb on + strandat 1916.926 kb on + strandat 1916.932 kb on - strandat 1917.354 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; DNA cleaned with chelator
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1,916,625 + SO1822 0.87 -0.2
1,916,633 - SO1822 0.87 -1.7
1,916,660 - SO1822 0.88 +1.5
1,916,796 - +1.2
1,916,864 + +0.0
1,916,924 + -0.2
1,916,926 + -1.0
1,916,932 - +0.8
1,917,354 + -0.8

Or see this region's nucleotide sequence