Experiment: Ferric citrate electron acceptor; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1188 and SO1189 are separated by 13 nucleotides SO1189 and SO1190 are separated by 29 nucleotides SO1190 and greA are separated by 264 nucleotides
SO1188: SO1188 - conserved hypothetical protein (NCBI ptt file), at 1,232,240 to 1,232,773
SO1188
SO1189: SO1189 - conserved hypothetical protein (NCBI ptt file), at 1,232,787 to 1,233,302
SO1189
SO1190: SO1190 - conserved hypothetical protein (NCBI ptt file), at 1,233,332 to 1,234,150
SO1190
SO1191: greA - transcription elongation factor GreA (NCBI ptt file), at 1,234,415 to 1,234,891
greA
Position (kb)
1233
1234
1235 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1232.348 kb on + strand, within SO1188 at 1232.429 kb on + strand, within SO1188 at 1232.511 kb on + strand, within SO1188 at 1232.511 kb on + strand, within SO1188 at 1232.511 kb on + strand, within SO1188 at 1232.616 kb on - strand, within SO1188 at 1232.617 kb on - strand, within SO1188 at 1232.723 kb on + strand at 1232.731 kb on - strand at 1232.836 kb on + strand at 1232.871 kb on + strand, within SO1189 at 1232.962 kb on + strand, within SO1189 at 1233.023 kb on + strand, within SO1189 at 1233.042 kb on + strand, within SO1189 at 1233.050 kb on - strand, within SO1189 at 1233.132 kb on + strand, within SO1189 at 1233.132 kb on + strand, within SO1189 at 1233.132 kb on + strand, within SO1189 at 1233.154 kb on - strand, within SO1189 at 1233.154 kb on - strand, within SO1189 at 1233.187 kb on - strand, within SO1189 at 1233.303 kb on + strand at 1233.311 kb on - strand at 1233.311 kb on - strand at 1233.779 kb on + strand, within SO1190 at 1233.932 kb on + strand, within SO1190 at 1234.062 kb on + strand, within SO1190 at 1234.062 kb on + strand, within SO1190 at 1234.070 kb on - strand at 1234.070 kb on - strand at 1234.070 kb on - strand at 1234.134 kb on + strand at 1234.139 kb on + strand at 1234.139 kb on + strand at 1234.332 kb on - strand at 1234.332 kb on - strand at 1234.366 kb on - strand at 1234.434 kb on - strand at 1234.451 kb on - strand at 1234.463 kb on + strand, within greA at 1234.474 kb on + strand, within greA at 1234.577 kb on - strand, within greA at 1234.928 kb on - strand at 1234.982 kb on + strand at 1235.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; DNA cleaned with chelator remove 1,232,348 + SO1188 0.20 -0.5 1,232,429 + SO1188 0.35 -2.7 1,232,511 + SO1188 0.51 -0.2 1,232,511 + SO1188 0.51 -0.5 1,232,511 + SO1188 0.51 +1.9 1,232,616 - SO1188 0.70 -1.0 1,232,617 - SO1188 0.71 -0.2 1,232,723 + -0.2 1,232,731 - -0.6 1,232,836 + -0.6 1,232,871 + SO1189 0.16 -3.1 1,232,962 + SO1189 0.34 +1.7 1,233,023 + SO1189 0.46 -0.2 1,233,042 + SO1189 0.49 +0.1 1,233,050 - SO1189 0.51 +0.9 1,233,132 + SO1189 0.67 +0.4 1,233,132 + SO1189 0.67 +0.1 1,233,132 + SO1189 0.67 -1.2 1,233,154 - SO1189 0.71 -0.2 1,233,154 - SO1189 0.71 +1.3 1,233,187 - SO1189 0.78 -1.0 1,233,303 + -0.6 1,233,311 - -1.7 1,233,311 - +0.1 1,233,779 + SO1190 0.55 -0.2 1,233,932 + SO1190 0.73 -1.9 1,234,062 + SO1190 0.89 -0.8 1,234,062 + SO1190 0.89 -0.6 1,234,070 - +0.1 1,234,070 - +0.1 1,234,070 - -1.5 1,234,134 + -0.4 1,234,139 + +0.1 1,234,139 + +1.7 1,234,332 - -0.6 1,234,332 - +0.8 1,234,366 - -0.2 1,234,434 - +0.1 1,234,451 - -0.3 1,234,463 + greA SO1191 0.10 -1.7 1,234,474 + greA SO1191 0.12 +0.3 1,234,577 - greA SO1191 0.34 -0.8 1,234,928 - +0.8 1,234,982 + +0.5 1,235,040 - -0.0
Or see this region's nucleotide sequence