Experiment: Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01265 and BWI76_RS01270 are separated by 88 nucleotides BWI76_RS01270 and BWI76_RS01275 overlap by 113 nucleotides BWI76_RS01275 and BWI76_RS01280 are separated by 232 nucleotides
BWI76_RS01265: BWI76_RS01265 - sugar/pyridoxal phosphate phosphatase YigL, at 259,119 to 259,919
_RS01265
BWI76_RS01270: BWI76_RS01270 - hypothetical protein, at 260,008 to 260,907
_RS01270
BWI76_RS01275: BWI76_RS01275 - LysR family transcriptional regulator, at 260,795 to 261,748
_RS01275
BWI76_RS01280: BWI76_RS01280 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 261,981 to 264,242
_RS01280
Position (kb) 260
261
262 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 259.806 kb on + strand, within BWI76_RS01265 at 259.846 kb on - strand at 260.025 kb on - strand at 260.056 kb on - strand at 260.125 kb on + strand, within BWI76_RS01270 at 260.163 kb on + strand, within BWI76_RS01270 at 260.184 kb on + strand, within BWI76_RS01270 at 260.206 kb on - strand, within BWI76_RS01270 at 260.450 kb on + strand, within BWI76_RS01270 at 260.451 kb on + strand, within BWI76_RS01270 at 260.459 kb on - strand, within BWI76_RS01270 at 260.522 kb on - strand, within BWI76_RS01270 at 260.522 kb on - strand, within BWI76_RS01270 at 260.578 kb on - strand, within BWI76_RS01270 at 260.996 kb on + strand, within BWI76_RS01275 at 261.005 kb on - strand, within BWI76_RS01275 at 261.109 kb on - strand, within BWI76_RS01275 at 261.114 kb on - strand, within BWI76_RS01275 at 261.435 kb on - strand, within BWI76_RS01275 at 261.625 kb on + strand, within BWI76_RS01275 at 261.633 kb on - strand, within BWI76_RS01275 at 261.663 kb on + strand at 261.699 kb on + strand at 261.904 kb on - strand at 262.023 kb on - strand at 262.089 kb on - strand at 262.183 kb on + strand at 262.201 kb on + strand at 262.226 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.517 kb on - strand, within BWI76_RS01280 at 262.692 kb on - strand, within BWI76_RS01280 at 262.692 kb on - strand, within BWI76_RS01280
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL remove 259,806 + BWI76_RS01265 0.86 +0.9 259,846 - -0.5 260,025 - -0.5 260,056 - +1.3 260,125 + BWI76_RS01270 0.13 -2.0 260,163 + BWI76_RS01270 0.17 -0.9 260,184 + BWI76_RS01270 0.20 +1.1 260,206 - BWI76_RS01270 0.22 +0.1 260,450 + BWI76_RS01270 0.49 +0.2 260,451 + BWI76_RS01270 0.49 -1.5 260,459 - BWI76_RS01270 0.50 +0.2 260,522 - BWI76_RS01270 0.57 +0.4 260,522 - BWI76_RS01270 0.57 -0.7 260,578 - BWI76_RS01270 0.63 -0.4 260,996 + BWI76_RS01275 0.21 -0.9 261,005 - BWI76_RS01275 0.22 -2.1 261,109 - BWI76_RS01275 0.33 -1.3 261,114 - BWI76_RS01275 0.33 -1.7 261,435 - BWI76_RS01275 0.67 -2.2 261,625 + BWI76_RS01275 0.87 -2.6 261,633 - BWI76_RS01275 0.88 -2.8 261,663 + -1.7 261,699 + -2.1 261,904 - -0.3 262,023 - -2.1 262,089 - -0.8 262,183 + -0.8 262,201 + -1.1 262,226 - BWI76_RS01280 0.11 -0.4 262,504 - BWI76_RS01280 0.23 +0.1 262,504 - BWI76_RS01280 0.23 -0.6 262,504 - BWI76_RS01280 0.23 +1.0 262,504 - BWI76_RS01280 0.23 -0.6 262,504 - BWI76_RS01280 0.23 -0.9 262,504 - BWI76_RS01280 0.23 -1.2 262,517 - BWI76_RS01280 0.24 -0.4 262,692 - BWI76_RS01280 0.31 -2.6 262,692 - BWI76_RS01280 0.31 -0.6
Or see this region's nucleotide sequence