Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21115 and BWI76_RS21120 are separated by 32 nucleotides BWI76_RS21120 and BWI76_RS21125 are separated by 96 nucleotides BWI76_RS21125 and BWI76_RS21130 are separated by 107 nucleotides BWI76_RS21130 and BWI76_RS21135 are separated by 1 nucleotides BWI76_RS21135 and BWI76_RS21140 are separated by 1 nucleotides
BWI76_RS21115: BWI76_RS21115 - 3-mercaptopyruvate sulfurtransferase, at 4,423,161 to 4,424,006
_RS21115
BWI76_RS21120: BWI76_RS21120 - enhanced serine sensitivity protein SseB, at 4,424,039 to 4,424,815
_RS21120
BWI76_RS21125: BWI76_RS21125 - aminopeptidase PepB, at 4,424,912 to 4,426,198
_RS21125
BWI76_RS21130: BWI76_RS21130 - Fe-S assembly protein IscX, at 4,426,306 to 4,426,506
_RS21130
BWI76_RS21135: BWI76_RS21135 - ferredoxin, 2Fe-2S type, ISC system, at 4,426,508 to 4,426,843
_RS21135
BWI76_RS21140: BWI76_RS21140 - molecular chaperone HscA, at 4,426,845 to 4,428,695
_RS21140
Position (kb) 4424
4425
4426
4427 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4423.980 kb on + strand at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.290 kb on - strand, within BWI76_RS21120 at 4424.290 kb on - strand at 4424.292 kb on - strand, within BWI76_RS21120 at 4424.384 kb on - strand, within BWI76_RS21120 at 4424.529 kb on + strand, within BWI76_RS21120 at 4424.681 kb on - strand, within BWI76_RS21120 at 4425.034 kb on - strand at 4425.226 kb on + strand, within BWI76_RS21125 at 4425.252 kb on + strand, within BWI76_RS21125 at 4425.260 kb on - strand, within BWI76_RS21125 at 4425.260 kb on - strand, within BWI76_RS21125 at 4425.315 kb on - strand, within BWI76_RS21125 at 4425.357 kb on - strand, within BWI76_RS21125 at 4425.734 kb on - strand, within BWI76_RS21125 at 4425.826 kb on + strand, within BWI76_RS21125 at 4425.899 kb on - strand, within BWI76_RS21125 at 4425.952 kb on - strand, within BWI76_RS21125 at 4426.228 kb on + strand at 4426.228 kb on + strand at 4426.228 kb on + strand at 4426.236 kb on - strand at 4426.236 kb on - strand at 4426.405 kb on - strand, within BWI76_RS21130 at 4426.454 kb on + strand, within BWI76_RS21130 at 4426.459 kb on + strand at 4426.490 kb on + strand at 4427.115 kb on - strand, within BWI76_RS21140
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,423,980 + +0.4 4,424,282 + BWI76_RS21120 0.31 +2.0 4,424,282 + BWI76_RS21120 0.31 +1.1 4,424,282 + BWI76_RS21120 0.31 +0.4 4,424,290 - BWI76_RS21120 0.32 +1.7 4,424,290 - -1.0 4,424,292 - BWI76_RS21120 0.33 -0.6 4,424,384 - BWI76_RS21120 0.44 +0.3 4,424,529 + BWI76_RS21120 0.63 +0.8 4,424,681 - BWI76_RS21120 0.83 +0.0 4,425,034 - -0.6 4,425,226 + BWI76_RS21125 0.24 +0.8 4,425,252 + BWI76_RS21125 0.26 +1.0 4,425,260 - BWI76_RS21125 0.27 +1.4 4,425,260 - BWI76_RS21125 0.27 -3.3 4,425,315 - BWI76_RS21125 0.31 +1.8 4,425,357 - BWI76_RS21125 0.35 +0.5 4,425,734 - BWI76_RS21125 0.64 -0.1 4,425,826 + BWI76_RS21125 0.71 -1.3 4,425,899 - BWI76_RS21125 0.77 +0.4 4,425,952 - BWI76_RS21125 0.81 -0.3 4,426,228 + -0.8 4,426,228 + +0.1 4,426,228 + +0.6 4,426,236 - -1.3 4,426,236 - +0.3 4,426,405 - BWI76_RS21130 0.49 +2.1 4,426,454 + BWI76_RS21130 0.74 +1.3 4,426,459 + +1.0 4,426,490 + -0.6 4,427,115 - BWI76_RS21140 0.15 +0.0
Or see this region's nucleotide sequence