Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20325 and BWI76_RS20330 overlap by 4 nucleotides BWI76_RS20330 and BWI76_RS20335 are separated by 66 nucleotides BWI76_RS20335 and BWI76_RS20340 are separated by 257 nucleotides
BWI76_RS20325: BWI76_RS20325 - histidine/lysine/arginine/ornithine ABC transporter permease HisM, at 4,255,553 to 4,256,269
_RS20325
BWI76_RS20330: BWI76_RS20330 - histidine/lysine/arginine/ornithine ABC transporter permease HisQ, at 4,256,266 to 4,256,952
_RS20330
BWI76_RS20335: BWI76_RS20335 - zinc transporter, at 4,257,019 to 4,257,801
_RS20335
BWI76_RS20340: BWI76_RS20340 - histidine ABC transporter substrate-binding protein HisJ, at 4,258,059 to 4,258,841
_RS20340
Position (kb) 4257
4258 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4256.054 kb on + strand, within BWI76_RS20325 at 4256.074 kb on + strand, within BWI76_RS20325 at 4256.271 kb on + strand at 4256.302 kb on - strand at 4256.322 kb on - strand at 4256.360 kb on + strand, within BWI76_RS20330 at 4256.425 kb on - strand, within BWI76_RS20330 at 4256.449 kb on + strand, within BWI76_RS20330 at 4256.722 kb on + strand, within BWI76_RS20330 at 4256.802 kb on - strand, within BWI76_RS20330 at 4256.898 kb on + strand at 4256.898 kb on + strand at 4256.906 kb on - strand at 4257.586 kb on - strand, within BWI76_RS20335 at 4257.598 kb on + strand, within BWI76_RS20335 at 4257.680 kb on + strand, within BWI76_RS20335 at 4257.705 kb on - strand, within BWI76_RS20335 at 4257.814 kb on - strand at 4258.239 kb on + strand, within BWI76_RS20340 at 4258.417 kb on - strand, within BWI76_RS20340 at 4258.419 kb on + strand, within BWI76_RS20340
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,256,054 + BWI76_RS20325 0.70 +1.2 4,256,074 + BWI76_RS20325 0.73 -0.9 4,256,271 + -1.0 4,256,302 - -0.9 4,256,322 - -0.8 4,256,360 + BWI76_RS20330 0.14 +0.1 4,256,425 - BWI76_RS20330 0.23 +1.6 4,256,449 + BWI76_RS20330 0.27 +2.2 4,256,722 + BWI76_RS20330 0.66 -0.6 4,256,802 - BWI76_RS20330 0.78 +0.6 4,256,898 + +0.8 4,256,898 + -2.0 4,256,906 - -0.3 4,257,586 - BWI76_RS20335 0.72 -3.1 4,257,598 + BWI76_RS20335 0.74 -1.5 4,257,680 + BWI76_RS20335 0.84 -2.0 4,257,705 - BWI76_RS20335 0.88 -1.0 4,257,814 - -1.5 4,258,239 + BWI76_RS20340 0.23 +0.8 4,258,417 - BWI76_RS20340 0.46 +0.4 4,258,419 + BWI76_RS20340 0.46 +0.7
Or see this region's nucleotide sequence