Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18820 and BWI76_RS18825 are separated by 39 nucleotides BWI76_RS18825 and BWI76_RS18830 are separated by 10 nucleotides BWI76_RS18830 and BWI76_RS18835 are separated by 322 nucleotides
BWI76_RS18820: BWI76_RS18820 - nitrogenase molybdenum-iron protein subunit beta, at 3,903,793 to 3,905,355
_RS18820
BWI76_RS18825: BWI76_RS18825 - protein NifT, at 3,905,395 to 3,905,613
_RS18825
BWI76_RS18830: BWI76_RS18830 - protein NifY, at 3,905,624 to 3,906,286
_RS18830
BWI76_RS18835: BWI76_RS18835 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 3,906,609 to 3,907,982
_RS18835
Position (kb)
3905
3906
3907 Strain fitness (log2 ratio)
-2
-1
0
1 at 3904.936 kb on + strand, within BWI76_RS18820 at 3905.033 kb on - strand, within BWI76_RS18820 at 3905.256 kb on - strand at 3905.274 kb on + strand at 3905.675 kb on + strand at 3905.848 kb on - strand, within BWI76_RS18830 at 3905.885 kb on + strand, within BWI76_RS18830 at 3906.358 kb on + strand at 3906.405 kb on + strand at 3906.470 kb on - strand at 3906.540 kb on + strand at 3906.749 kb on + strand, within BWI76_RS18835 at 3906.881 kb on + strand, within BWI76_RS18835 at 3906.922 kb on - strand, within BWI76_RS18835 at 3907.024 kb on - strand, within BWI76_RS18835 at 3907.049 kb on + strand, within BWI76_RS18835
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,904,936 + BWI76_RS18820 0.73 +0.2 3,905,033 - BWI76_RS18820 0.79 +0.4 3,905,256 - +0.4 3,905,274 + +0.2 3,905,675 + +0.1 3,905,848 - BWI76_RS18830 0.34 +0.3 3,905,885 + BWI76_RS18830 0.39 +1.4 3,906,358 + +0.3 3,906,405 + -2.2 3,906,470 - -2.5 3,906,540 + +1.0 3,906,749 + BWI76_RS18835 0.10 +0.5 3,906,881 + BWI76_RS18835 0.20 +0.1 3,906,922 - BWI76_RS18835 0.23 +0.9 3,907,024 - BWI76_RS18835 0.30 -0.6 3,907,049 + BWI76_RS18835 0.32 +0.1
Or see this region's nucleotide sequence