Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16205 and BWI76_RS16210 are separated by 343 nucleotides BWI76_RS16210 and BWI76_RS16215 overlap by 4 nucleotides BWI76_RS16215 and BWI76_RS16220 overlap by 23 nucleotides
BWI76_RS16205: BWI76_RS16205 - NAD(P) transhydrogenase subunit alpha, at 3,362,286 to 3,363,815
_RS16205
BWI76_RS16210: BWI76_RS16210 - hypothetical protein, at 3,364,159 to 3,364,341
_RS16210
BWI76_RS16215: BWI76_RS16215 - hypothetical protein, at 3,364,338 to 3,365,288
_RS16215
BWI76_RS16220: BWI76_RS16220 - hypothetical protein, at 3,365,266 to 3,365,468
_RS16220
Position (kb)
3364
3365 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3363.199 kb on + strand, within BWI76_RS16205 at 3363.293 kb on + strand, within BWI76_RS16205 at 3363.315 kb on - strand, within BWI76_RS16205 at 3363.786 kb on - strand at 3363.893 kb on + strand at 3364.072 kb on + strand at 3364.250 kb on + strand, within BWI76_RS16210 at 3364.318 kb on - strand, within BWI76_RS16210 at 3364.356 kb on + strand at 3364.364 kb on - strand at 3364.458 kb on + strand, within BWI76_RS16215 at 3364.581 kb on - strand, within BWI76_RS16215 at 3364.813 kb on + strand, within BWI76_RS16215 at 3364.835 kb on + strand, within BWI76_RS16215 at 3365.240 kb on + strand at 3365.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,363,199 + BWI76_RS16205 0.60 +0.5 3,363,293 + BWI76_RS16205 0.66 -0.3 3,363,315 - BWI76_RS16205 0.67 -1.5 3,363,786 - -0.3 3,363,893 + -1.0 3,364,072 + -2.9 3,364,250 + BWI76_RS16210 0.50 -1.7 3,364,318 - BWI76_RS16210 0.87 -3.0 3,364,356 + +0.0 3,364,364 - +2.0 3,364,458 + BWI76_RS16215 0.13 +0.3 3,364,581 - BWI76_RS16215 0.26 +0.9 3,364,813 + BWI76_RS16215 0.50 -0.9 3,364,835 + BWI76_RS16215 0.52 +0.9 3,365,240 + -1.3 3,365,251 - -1.8
Or see this region's nucleotide sequence