Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13315

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS13310 and BWI76_RS13315 are separated by 75 nucleotidesBWI76_RS13315 and BWI76_RS13320 are separated by 95 nucleotides BWI76_RS13310: BWI76_RS13310 - glucan biosynthesis protein D, at 2,775,559 to 2,777,214 _RS13310 BWI76_RS13315: BWI76_RS13315 - 50S ribosomal protein L7/L12-serine acetyltransferase, at 2,777,290 to 2,777,874 _RS13315 BWI76_RS13320: BWI76_RS13320 - putative LpxA-like enzyme, at 2,777,970 to 2,778,950 _RS13320 Position (kb) 2777 2778Strain fitness (log2 ratio) -3 -2 -1 0 1at 2776.573 kb on - strand, within BWI76_RS13310at 2777.103 kb on - strandat 2777.126 kb on - strandat 2777.128 kb on - strandat 2777.367 kb on - strand, within BWI76_RS13315at 2778.638 kb on + strand, within BWI76_RS13320at 2778.797 kb on - strand, within BWI76_RS13320

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
remove
2,776,573 - BWI76_RS13310 0.61 +1.0
2,777,103 - -3.5
2,777,126 - +0.7
2,777,128 - -1.2
2,777,367 - BWI76_RS13315 0.13 -2.3
2,778,638 + BWI76_RS13320 0.68 -0.1
2,778,797 - BWI76_RS13320 0.84 +1.7

Or see this region's nucleotide sequence