Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08265 and BWI76_RS08270 are separated by 291 nucleotides BWI76_RS08270 and BWI76_RS08275 are separated by 50 nucleotides BWI76_RS08275 and BWI76_RS08280 are separated by 133 nucleotides
BWI76_RS08265: BWI76_RS08265 - transcriptional repressor, at 1,750,135 to 1,750,587
_RS08265
BWI76_RS08270: BWI76_RS08270 - flavodoxin, at 1,750,879 to 1,751,508
_RS08270
BWI76_RS08275: BWI76_RS08275 - LexA regulated protein, at 1,751,559 to 1,751,852
_RS08275
BWI76_RS08280: BWI76_RS08280 - acyl-CoA esterase, at 1,751,986 to 1,752,759
_RS08280
Position (kb)
1751
1752 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1750.688 kb on + strand at 1750.810 kb on + strand at 1751.518 kb on + strand at 1751.526 kb on - strand at 1751.571 kb on - strand at 1751.722 kb on + strand, within BWI76_RS08275 at 1751.754 kb on - strand, within BWI76_RS08275 at 1752.008 kb on - strand at 1752.163 kb on - strand, within BWI76_RS08280 at 1752.589 kb on + strand, within BWI76_RS08280 at 1752.605 kb on - strand, within BWI76_RS08280
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,750,688 + +0.3 1,750,810 + -0.3 1,751,518 + +0.3 1,751,526 - +0.4 1,751,571 - -3.5 1,751,722 + BWI76_RS08275 0.55 -2.9 1,751,754 - BWI76_RS08275 0.66 -1.5 1,752,008 - +0.5 1,752,163 - BWI76_RS08280 0.23 +0.5 1,752,589 + BWI76_RS08280 0.78 +0.6 1,752,605 - BWI76_RS08280 0.80 +1.0
Or see this region's nucleotide sequence