Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04420

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04415 and BWI76_RS04420 are separated by 12 nucleotidesBWI76_RS04420 and BWI76_RS04425 are separated by 15 nucleotidesBWI76_RS04425 and BWI76_RS04430 are separated by 160 nucleotides BWI76_RS04415: BWI76_RS04415 - oxaloacetate decarboxylase subunit beta, at 911,208 to 912,509 _RS04415 BWI76_RS04420: BWI76_RS04420 - oxaloacetate decarboxylase, at 912,522 to 914,288 _RS04420 BWI76_RS04425: BWI76_RS04425 - oxaloacetate decarboxylase subunit gamma, at 914,304 to 914,555 _RS04425 BWI76_RS04430: BWI76_RS04430 - citrate carrier protein, at 914,716 to 916,056 _RS04430 Position (kb) 912 913 914 915Strain fitness (log2 ratio) -3 -2 -1 0 1at 912.549 kb on + strandat 912.731 kb on + strand, within BWI76_RS04420at 912.828 kb on + strand, within BWI76_RS04420at 912.852 kb on + strand, within BWI76_RS04420at 913.020 kb on + strand, within BWI76_RS04420at 913.157 kb on + strand, within BWI76_RS04420at 913.533 kb on - strand, within BWI76_RS04420at 913.647 kb on - strand, within BWI76_RS04420at 913.825 kb on - strand, within BWI76_RS04420at 913.835 kb on - strand, within BWI76_RS04420at 913.863 kb on - strand, within BWI76_RS04420at 914.101 kb on + strandat 914.511 kb on + strand, within BWI76_RS04425at 914.540 kb on - strandat 914.630 kb on - strandat 914.820 kb on - strandat 914.820 kb on - strandat 914.836 kb on - strandat 914.861 kb on + strand, within BWI76_RS04430at 914.866 kb on - strand, within BWI76_RS04430at 914.868 kb on + strand, within BWI76_RS04430at 915.014 kb on - strand, within BWI76_RS04430at 915.019 kb on - strand, within BWI76_RS04430at 915.121 kb on + strand, within BWI76_RS04430at 915.129 kb on - strand, within BWI76_RS04430at 915.136 kb on + strand, within BWI76_RS04430at 915.214 kb on - strand, within BWI76_RS04430at 915.244 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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912,549 + -2.9
912,731 + BWI76_RS04420 0.12 -0.3
912,828 + BWI76_RS04420 0.17 -2.1
912,852 + BWI76_RS04420 0.19 -1.9
913,020 + BWI76_RS04420 0.28 -0.2
913,157 + BWI76_RS04420 0.36 +0.0
913,533 - BWI76_RS04420 0.57 -3.0
913,647 - BWI76_RS04420 0.64 -0.9
913,825 - BWI76_RS04420 0.74 -0.0
913,835 - BWI76_RS04420 0.74 +0.2
913,863 - BWI76_RS04420 0.76 -0.4
914,101 + -1.9
914,511 + BWI76_RS04425 0.82 -0.3
914,540 - -3.4
914,630 - -3.4
914,820 - +1.1
914,820 - +0.7
914,836 - +0.3
914,861 + BWI76_RS04430 0.11 -1.3
914,866 - BWI76_RS04430 0.11 +0.1
914,868 + BWI76_RS04430 0.11 +0.1
915,014 - BWI76_RS04430 0.22 +0.4
915,019 - BWI76_RS04430 0.23 -0.2
915,121 + BWI76_RS04430 0.30 -0.5
915,129 - BWI76_RS04430 0.31 -0.4
915,136 + BWI76_RS04430 0.31 -1.0
915,214 - BWI76_RS04430 0.37 -1.5
915,244 + +0.1

Or see this region's nucleotide sequence