Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04400 and BWI76_RS04405 are separated by 44 nucleotides BWI76_RS04405 and BWI76_RS04410 overlap by 8 nucleotides BWI76_RS04410 and BWI76_RS04415 are separated by 246 nucleotides
BWI76_RS04400: BWI76_RS04400 - alanyl-tRNA synthetase, at 907,968 to 908,597
_RS04400
BWI76_RS04405: BWI76_RS04405 - response regulator, at 908,642 to 909,340
_RS04405
BWI76_RS04410: BWI76_RS04410 - signal transduction histidine kinase regulating citrate/malate metabolism, at 909,333 to 910,961
_RS04410
BWI76_RS04415: BWI76_RS04415 - oxaloacetate decarboxylase subunit beta, at 911,208 to 912,509
_RS04415
Position (kb) 909
910
911 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 908.336 kb on - strand, within BWI76_RS04400 at 908.365 kb on - strand, within BWI76_RS04400 at 908.497 kb on - strand, within BWI76_RS04400 at 908.509 kb on + strand, within BWI76_RS04400 at 908.595 kb on - strand at 908.740 kb on + strand, within BWI76_RS04405 at 908.753 kb on - strand, within BWI76_RS04405 at 908.753 kb on - strand, within BWI76_RS04405 at 908.836 kb on - strand, within BWI76_RS04405 at 908.843 kb on + strand, within BWI76_RS04405 at 908.850 kb on - strand, within BWI76_RS04405 at 908.855 kb on + strand, within BWI76_RS04405 at 908.910 kb on + strand, within BWI76_RS04405 at 908.956 kb on + strand, within BWI76_RS04405 at 909.112 kb on + strand, within BWI76_RS04405 at 909.170 kb on - strand, within BWI76_RS04405 at 909.186 kb on + strand, within BWI76_RS04405 at 909.204 kb on - strand, within BWI76_RS04405 at 909.238 kb on - strand, within BWI76_RS04405 at 909.257 kb on - strand, within BWI76_RS04405 at 909.273 kb on + strand at 909.298 kb on + strand at 909.306 kb on - strand at 909.325 kb on + strand at 909.333 kb on - strand at 909.447 kb on - strand at 909.449 kb on + strand at 909.599 kb on + strand, within BWI76_RS04410 at 909.607 kb on - strand, within BWI76_RS04410 at 909.699 kb on + strand, within BWI76_RS04410 at 909.700 kb on - strand, within BWI76_RS04410 at 909.707 kb on - strand, within BWI76_RS04410 at 909.708 kb on - strand, within BWI76_RS04410 at 909.731 kb on + strand, within BWI76_RS04410 at 909.739 kb on - strand, within BWI76_RS04410 at 909.754 kb on + strand, within BWI76_RS04410 at 909.832 kb on - strand, within BWI76_RS04410 at 909.843 kb on - strand, within BWI76_RS04410 at 910.034 kb on - strand, within BWI76_RS04410 at 910.034 kb on - strand, within BWI76_RS04410 at 910.080 kb on - strand, within BWI76_RS04410 at 910.082 kb on + strand, within BWI76_RS04410 at 910.082 kb on + strand, within BWI76_RS04410 at 910.090 kb on - strand, within BWI76_RS04410 at 910.207 kb on - strand, within BWI76_RS04410 at 910.313 kb on + strand, within BWI76_RS04410 at 910.380 kb on + strand, within BWI76_RS04410 at 910.472 kb on + strand, within BWI76_RS04410 at 910.532 kb on + strand, within BWI76_RS04410 at 910.532 kb on + strand, within BWI76_RS04410 at 910.584 kb on - strand, within BWI76_RS04410 at 910.658 kb on - strand, within BWI76_RS04410
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 908,336 - BWI76_RS04400 0.58 -3.3 908,365 - BWI76_RS04400 0.63 +1.1 908,497 - BWI76_RS04400 0.84 +0.4 908,509 + BWI76_RS04400 0.86 -1.3 908,595 - -0.9 908,740 + BWI76_RS04405 0.14 +1.1 908,753 - BWI76_RS04405 0.16 +0.1 908,753 - BWI76_RS04405 0.16 -1.2 908,836 - BWI76_RS04405 0.28 -2.6 908,843 + BWI76_RS04405 0.29 +0.1 908,850 - BWI76_RS04405 0.30 -3.3 908,855 + BWI76_RS04405 0.30 -0.7 908,910 + BWI76_RS04405 0.38 -0.4 908,956 + BWI76_RS04405 0.45 -0.1 909,112 + BWI76_RS04405 0.67 +0.7 909,170 - BWI76_RS04405 0.76 -0.1 909,186 + BWI76_RS04405 0.78 +0.1 909,204 - BWI76_RS04405 0.80 +0.3 909,238 - BWI76_RS04405 0.85 +2.4 909,257 - BWI76_RS04405 0.88 -0.8 909,273 + -0.1 909,298 + -0.5 909,306 - +0.3 909,325 + -0.7 909,333 - -0.0 909,447 - -1.3 909,449 + +1.2 909,599 + BWI76_RS04410 0.16 +0.5 909,607 - BWI76_RS04410 0.17 +0.2 909,699 + BWI76_RS04410 0.22 +1.9 909,700 - BWI76_RS04410 0.23 +0.6 909,707 - BWI76_RS04410 0.23 +3.7 909,708 - BWI76_RS04410 0.23 +1.0 909,731 + BWI76_RS04410 0.24 -0.3 909,739 - BWI76_RS04410 0.25 -0.2 909,754 + BWI76_RS04410 0.26 +1.6 909,832 - BWI76_RS04410 0.31 +0.8 909,843 - BWI76_RS04410 0.31 +0.3 910,034 - BWI76_RS04410 0.43 +0.8 910,034 - BWI76_RS04410 0.43 +0.7 910,080 - BWI76_RS04410 0.46 +1.1 910,082 + BWI76_RS04410 0.46 +1.4 910,082 + BWI76_RS04410 0.46 +0.6 910,090 - BWI76_RS04410 0.46 -0.1 910,207 - BWI76_RS04410 0.54 +0.5 910,313 + BWI76_RS04410 0.60 +0.4 910,380 + BWI76_RS04410 0.64 +0.4 910,472 + BWI76_RS04410 0.70 -2.0 910,532 + BWI76_RS04410 0.74 +0.4 910,532 + BWI76_RS04410 0.74 +0.5 910,584 - BWI76_RS04410 0.77 +1.1 910,658 - BWI76_RS04410 0.81 +0.2
Or see this region's nucleotide sequence