Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27380 and BWI76_RS27385 are separated by 124 nucleotides BWI76_RS27385 and BWI76_RS27390 are separated by 65 nucleotides BWI76_RS27390 and BWI76_RS27395 are separated by 123 nucleotides
BWI76_RS27380: BWI76_RS27380 - nucleoid occlusion factor SlmA, at 5,681,678 to 5,682,274
_RS27380
BWI76_RS27385: BWI76_RS27385 - orotate phosphoribosyltransferase, at 5,682,399 to 5,683,040
_RS27385
BWI76_RS27390: BWI76_RS27390 - ribonuclease PH, at 5,683,106 to 5,683,822
_RS27390
BWI76_RS27395: BWI76_RS27395 - hypothetical protein, at 5,683,946 to 5,684,809
_RS27395
Position (kb) 5683
5684 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5682.119 kb on + strand, within BWI76_RS27380 at 5682.161 kb on + strand, within BWI76_RS27380 at 5682.193 kb on - strand, within BWI76_RS27380 at 5682.350 kb on + strand at 5682.357 kb on + strand at 5682.423 kb on + strand at 5682.695 kb on - strand, within BWI76_RS27385 at 5682.695 kb on - strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.924 kb on - strand, within BWI76_RS27385 at 5682.924 kb on - strand, within BWI76_RS27385 at 5682.924 kb on - strand, within BWI76_RS27385 at 5683.011 kb on + strand at 5683.056 kb on - strand at 5683.142 kb on + strand at 5683.155 kb on + strand at 5683.194 kb on - strand, within BWI76_RS27390 at 5683.328 kb on + strand, within BWI76_RS27390 at 5683.468 kb on + strand, within BWI76_RS27390 at 5683.607 kb on + strand, within BWI76_RS27390 at 5683.652 kb on + strand, within BWI76_RS27390 at 5683.655 kb on - strand, within BWI76_RS27390 at 5683.719 kb on - strand, within BWI76_RS27390 at 5683.738 kb on + strand, within BWI76_RS27390 at 5683.772 kb on + strand at 5683.772 kb on + strand at 5683.780 kb on - strand at 5683.780 kb on - strand at 5683.798 kb on - strand at 5683.905 kb on + strand at 5683.913 kb on - strand at 5683.941 kb on + strand at 5683.941 kb on + strand at 5684.004 kb on - strand at 5684.106 kb on + strand, within BWI76_RS27395 at 5684.170 kb on + strand, within BWI76_RS27395 at 5684.178 kb on - strand, within BWI76_RS27395 at 5684.544 kb on + strand, within BWI76_RS27395 at 5684.565 kb on + strand, within BWI76_RS27395
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 5,682,119 + BWI76_RS27380 0.74 -0.6 5,682,161 + BWI76_RS27380 0.81 +0.1 5,682,193 - BWI76_RS27380 0.86 -0.1 5,682,350 + +0.7 5,682,357 + -0.3 5,682,423 + -0.9 5,682,695 - BWI76_RS27385 0.46 +2.5 5,682,695 - BWI76_RS27385 0.46 -3.5 5,682,916 + BWI76_RS27385 0.81 +0.9 5,682,916 + BWI76_RS27385 0.81 -1.5 5,682,916 + BWI76_RS27385 0.81 -0.5 5,682,916 + BWI76_RS27385 0.81 +0.1 5,682,916 + BWI76_RS27385 0.81 -1.0 5,682,916 + BWI76_RS27385 0.81 -1.2 5,682,916 + BWI76_RS27385 0.81 -1.6 5,682,924 - BWI76_RS27385 0.82 -1.2 5,682,924 - BWI76_RS27385 0.82 -1.4 5,682,924 - BWI76_RS27385 0.82 +0.9 5,683,011 + -1.8 5,683,056 - -1.2 5,683,142 + -0.6 5,683,155 + -2.5 5,683,194 - BWI76_RS27390 0.12 -0.3 5,683,328 + BWI76_RS27390 0.31 -2.5 5,683,468 + BWI76_RS27390 0.50 -0.9 5,683,607 + BWI76_RS27390 0.70 -2.0 5,683,652 + BWI76_RS27390 0.76 -0.5 5,683,655 - BWI76_RS27390 0.77 -0.1 5,683,719 - BWI76_RS27390 0.85 -0.6 5,683,738 + BWI76_RS27390 0.88 -2.5 5,683,772 + -2.0 5,683,772 + -3.1 5,683,780 - -0.4 5,683,780 - -0.5 5,683,798 - -1.6 5,683,905 + -0.0 5,683,913 - +2.3 5,683,941 + -0.6 5,683,941 + -1.6 5,684,004 - -0.3 5,684,106 + BWI76_RS27395 0.19 +1.5 5,684,170 + BWI76_RS27395 0.26 -0.1 5,684,178 - BWI76_RS27395 0.27 -1.4 5,684,544 + BWI76_RS27395 0.69 -0.0 5,684,565 + BWI76_RS27395 0.72 -1.1
Or see this region's nucleotide sequence