Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16000

Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
3,318,657	-		BWI76_RS15995	0.69	-0.1
3,318,764	+		BWI76_RS15995	0.73	+0.6
3,319,058	+		BWI76_RS15995	0.87	+0.4
3,319,081	-		BWI76_RS15995	0.88	-0.1
3,319,081	-		BWI76_RS15995	0.88	+0.7
3,319,081	-		BWI76_RS15995	0.88	-0.0
3,319,112	+		BWI76_RS15995	0.90	-1.3
3,319,200	+				+0.2
3,319,226	+				+1.7
3,319,229	-				-0.2
3,319,336	+				-0.1
3,319,341	+				+0.3
3,319,346	+				+1.3
3,319,392	+				+0.4
3,319,392	+				+0.2
3,319,400	-				-2.2
3,319,400	-				+1.3
3,319,402	+				+1.0
3,319,580	-		BWI76_RS16000	0.42	-1.3
3,319,739	-				+0.6
3,319,739	-				-0.4
3,319,745	+				-0.6
3,319,754	+				+0.2
3,319,758	-				-0.5
3,319,762	-				+3.7
3,319,830	+		BWI76_RS16005	0.20	-1.1
3,319,839	+				-0.1
3,319,852	-		BWI76_RS16005	0.26	+0.4
3,319,886	+		BWI76_RS16005	0.36	+1.3
3,319,888	+		BWI76_RS16005	0.36	+0.4
3,319,888	+		BWI76_RS16005	0.36	-1.7
3,319,896	-		BWI76_RS16005	0.38	+0.6
3,319,912	+		BWI76_RS16005	0.43	+0.0
3,319,912	+		BWI76_RS16005	0.43	-1.2
3,319,920	-		BWI76_RS16005	0.45	+0.2
3,319,920	-		BWI76_RS16005	0.45	+0.2
3,319,920	-		BWI76_RS16005	0.45	-0.3
3,319,920	-		BWI76_RS16005	0.45	+0.5
3,319,943	+		BWI76_RS16005	0.51	+0.7
3,319,944	-		BWI76_RS16005	0.52	-1.8
3,319,944	-		BWI76_RS16005	0.52	-2.9
3,319,944	-		BWI76_RS16005	0.52	-0.1
3,319,945	-		BWI76_RS16005	0.52	-0.0
3,319,951	-		BWI76_RS16005	0.53	-0.4
3,319,951	-		BWI76_RS16005	0.53	-0.2
3,319,952	-		BWI76_RS16005	0.54	+0.3
3,319,952	-		BWI76_RS16005	0.54	-0.1
3,320,123	+				+1.2
3,320,131	-				-1.5
3,320,131	-				-0.0
3,320,156	-				-0.5
3,320,214	-		BWI76_RS16010	0.20	-1.4
3,320,223	-		BWI76_RS16010	0.23	-0.1
3,320,223	-		BWI76_RS16010	0.23	-0.9
3,320,227	+		BWI76_RS16010	0.24	-0.1
3,320,229	+		BWI76_RS16010	0.24	-0.3
3,320,242	-		BWI76_RS16010	0.28	-1.1
3,320,245	-		BWI76_RS16010	0.29	+0.0
3,320,258	-		BWI76_RS16010	0.33	+0.3
3,320,283	+		BWI76_RS16010	0.41	-0.7
3,320,285	-		BWI76_RS16010	0.42	-1.4
3,320,301	-		BWI76_RS16010	0.47	+0.4
3,320,405	-		BWI76_RS16010	0.79	-2.4
3,320,411	+		BWI76_RS16010	0.81	-0.7
3,320,419	-		BWI76_RS16010	0.83	-0.3
3,320,425	+		BWI76_RS16010	0.85	+0.9
3,320,432	-		BWI76_RS16010	0.87	-2.3
3,320,460	-				-0.1
3,320,594	+				-1.1
3,320,594	+				+0.3
3,320,594	+				+0.8
3,320,594	+				-0.6
3,320,594	+				+0.0
3,320,595	+				-1.1
3,320,599	+				+1.0
3,320,602	-				-0.0
3,320,602	-				-0.2
3,320,602	-				-0.4
3,320,602	-				+0.8
3,320,602	-				-0.3
3,320,602	-				-1.0
3,320,602	-				+0.8
3,320,603	-				+0.3
3,320,613	-				-1.0
3,320,638	+				-0.2
3,320,648	+				-0.1
3,320,704	+				+0.6
3,320,713	+				+1.1
3,320,728	-				+2.3