Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14300

Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14295 and BWI76_RS14300 overlap by 72 nucleotidesBWI76_RS14300 and BWI76_RS14305 are separated by 87 nucleotidesBWI76_RS14305 and BWI76_RS14310 are separated by 4 nucleotides BWI76_RS14295: BWI76_RS14295 - IS630 family transposase, at 2,988,739 to 2,989,680 _RS14295 BWI76_RS14300: BWI76_RS14300 - hypothetical protein, at 2,989,609 to 2,989,795 _RS14300 BWI76_RS14305: BWI76_RS14305 - metallophosphoesterase, at 2,989,883 to 2,990,668 _RS14305 BWI76_RS14310: BWI76_RS14310 - hypothetical protein, at 2,990,673 to 2,991,554 _RS14310 Position (kb) 2989 2990Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2988.621 kb on - strandat 2988.808 kb on + strandat 2988.840 kb on + strand, within BWI76_RS14295at 2988.999 kb on - strand, within BWI76_RS14295at 2989.053 kb on - strand, within BWI76_RS14295at 2989.125 kb on - strand, within BWI76_RS14295at 2989.275 kb on + strand, within BWI76_RS14295at 2989.275 kb on + strand, within BWI76_RS14295at 2989.275 kb on + strand, within BWI76_RS14295at 2989.283 kb on - strand, within BWI76_RS14295at 2989.283 kb on - strand, within BWI76_RS14295at 2989.286 kb on + strand, within BWI76_RS14295at 2989.294 kb on - strand, within BWI76_RS14295at 2989.452 kb on + strand, within BWI76_RS14295at 2989.453 kb on + strand, within BWI76_RS14295at 2989.454 kb on + strand, within BWI76_RS14295at 2989.462 kb on - strand, within BWI76_RS14295at 2989.502 kb on + strand, within BWI76_RS14295at 2989.535 kb on - strand, within BWI76_RS14295at 2989.665 kb on + strandat 2989.670 kb on + strandat 2989.701 kb on + strand, within BWI76_RS14300at 2989.715 kb on - strand, within BWI76_RS14300at 2989.728 kb on + strand, within BWI76_RS14300at 2989.731 kb on - strand, within BWI76_RS14300at 2989.731 kb on - strandat 2989.801 kb on + strandat 2989.806 kb on + strandat 2990.042 kb on + strand, within BWI76_RS14305at 2990.047 kb on + strand, within BWI76_RS14305at 2990.321 kb on + strand, within BWI76_RS14305at 2990.390 kb on + strand, within BWI76_RS14305at 2990.405 kb on + strand, within BWI76_RS14305at 2990.471 kb on + strand, within BWI76_RS14305at 2990.471 kb on + strand, within BWI76_RS14305at 2990.513 kb on + strand, within BWI76_RS14305at 2990.513 kb on + strand, within BWI76_RS14305

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,988,621 - +0.5
2,988,808 + +0.5
2,988,840 + BWI76_RS14295 0.11 -0.4
2,988,999 - BWI76_RS14295 0.28 -0.1
2,989,053 - BWI76_RS14295 0.33 +0.6
2,989,125 - BWI76_RS14295 0.41 -0.5
2,989,275 + BWI76_RS14295 0.57 -0.3
2,989,275 + BWI76_RS14295 0.57 -1.1
2,989,275 + BWI76_RS14295 0.57 +0.1
2,989,283 - BWI76_RS14295 0.58 +2.1
2,989,283 - BWI76_RS14295 0.58 +0.1
2,989,286 + BWI76_RS14295 0.58 +0.7
2,989,294 - BWI76_RS14295 0.59 +0.5
2,989,452 + BWI76_RS14295 0.76 -1.6
2,989,453 + BWI76_RS14295 0.76 +0.4
2,989,454 + BWI76_RS14295 0.76 -0.8
2,989,462 - BWI76_RS14295 0.77 -2.6
2,989,502 + BWI76_RS14295 0.81 +0.1
2,989,535 - BWI76_RS14295 0.85 -2.3
2,989,665 + +0.7
2,989,670 + +0.7
2,989,701 + BWI76_RS14300 0.49 -2.3
2,989,715 - BWI76_RS14300 0.57 -0.2
2,989,728 + BWI76_RS14300 0.64 -1.4
2,989,731 - BWI76_RS14300 0.65 +0.5
2,989,731 - +1.1
2,989,801 + +0.8
2,989,806 + -0.3
2,990,042 + BWI76_RS14305 0.20 -0.3
2,990,047 + BWI76_RS14305 0.21 -1.2
2,990,321 + BWI76_RS14305 0.56 -0.2
2,990,390 + BWI76_RS14305 0.65 -1.5
2,990,405 + BWI76_RS14305 0.66 -2.5
2,990,471 + BWI76_RS14305 0.75 -1.0
2,990,471 + BWI76_RS14305 0.75 +0.8
2,990,513 + BWI76_RS14305 0.80 -1.5
2,990,513 + BWI76_RS14305 0.80 +2.8

Or see this region's nucleotide sequence