Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14240

Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14235 and BWI76_RS14240 are separated by 43 nucleotidesBWI76_RS14240 and BWI76_RS14245 are separated by 35 nucleotidesBWI76_RS14245 and BWI76_RS14250 are separated by 111 nucleotides BWI76_RS14235: BWI76_RS14235 - alcohol dehydrogenase, at 2,978,303 to 2,979,327 _RS14235 BWI76_RS14240: BWI76_RS14240 - thiol reductase thioredoxin, at 2,979,371 to 2,979,694 _RS14240 BWI76_RS14245: BWI76_RS14245 - carboxymuconolactone decarboxylase family protein, at 2,979,730 to 2,980,251 _RS14245 BWI76_RS14250: BWI76_RS14250 - cytochrome ubiquinol oxidase subunit I, at 2,980,363 to 2,981,781 _RS14250 Position (kb) 2979 2980Strain fitness (log2 ratio) -3 -2 -1 0 1at 2978.403 kb on - strandat 2978.449 kb on - strand, within BWI76_RS14235at 2978.546 kb on - strand, within BWI76_RS14235at 2978.552 kb on - strand, within BWI76_RS14235at 2978.552 kb on - strand, within BWI76_RS14235at 2978.702 kb on - strand, within BWI76_RS14235at 2978.744 kb on + strand, within BWI76_RS14235at 2978.892 kb on + strand, within BWI76_RS14235at 2978.900 kb on - strand, within BWI76_RS14235at 2978.940 kb on + strand, within BWI76_RS14235at 2979.367 kb on + strandat 2979.468 kb on - strand, within BWI76_RS14240at 2979.563 kb on - strand, within BWI76_RS14240at 2979.626 kb on + strand, within BWI76_RS14240at 2979.634 kb on - strand, within BWI76_RS14240at 2979.743 kb on + strandat 2979.796 kb on + strand, within BWI76_RS14245at 2979.926 kb on + strand, within BWI76_RS14245at 2979.943 kb on - strand, within BWI76_RS14245at 2979.990 kb on - strand, within BWI76_RS14245at 2980.261 kb on + strandat 2980.330 kb on - strandat 2980.581 kb on + strandat 2980.589 kb on - strand, within BWI76_RS14250

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,978,403 - +1.1
2,978,449 - BWI76_RS14235 0.14 +0.0
2,978,546 - BWI76_RS14235 0.24 +0.3
2,978,552 - BWI76_RS14235 0.24 +1.6
2,978,552 - BWI76_RS14235 0.24 -0.1
2,978,702 - BWI76_RS14235 0.39 +0.2
2,978,744 + BWI76_RS14235 0.43 +0.4
2,978,892 + BWI76_RS14235 0.57 -0.5
2,978,900 - BWI76_RS14235 0.58 -2.5
2,978,940 + BWI76_RS14235 0.62 -1.2
2,979,367 + +1.1
2,979,468 - BWI76_RS14240 0.30 -0.9
2,979,563 - BWI76_RS14240 0.59 -3.0
2,979,626 + BWI76_RS14240 0.79 +0.2
2,979,634 - BWI76_RS14240 0.81 -3.5
2,979,743 + -0.9
2,979,796 + BWI76_RS14245 0.13 -1.3
2,979,926 + BWI76_RS14245 0.38 +0.3
2,979,943 - BWI76_RS14245 0.41 -0.4
2,979,990 - BWI76_RS14245 0.50 -0.9
2,980,261 + -0.0
2,980,330 - -0.1
2,980,581 + -0.2
2,980,589 - BWI76_RS14250 0.16 -0.9

Or see this region's nucleotide sequence