Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14235 and BWI76_RS14240 are separated by 43 nucleotides BWI76_RS14240 and BWI76_RS14245 are separated by 35 nucleotides BWI76_RS14245 and BWI76_RS14250 are separated by 111 nucleotides
BWI76_RS14235: BWI76_RS14235 - alcohol dehydrogenase, at 2,978,303 to 2,979,327
_RS14235
BWI76_RS14240: BWI76_RS14240 - thiol reductase thioredoxin, at 2,979,371 to 2,979,694
_RS14240
BWI76_RS14245: BWI76_RS14245 - carboxymuconolactone decarboxylase family protein, at 2,979,730 to 2,980,251
_RS14245
BWI76_RS14250: BWI76_RS14250 - cytochrome ubiquinol oxidase subunit I, at 2,980,363 to 2,981,781
_RS14250
Position (kb)
2979
2980 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2978.403 kb on - strand at 2978.449 kb on - strand, within BWI76_RS14235 at 2978.546 kb on - strand, within BWI76_RS14235 at 2978.552 kb on - strand, within BWI76_RS14235 at 2978.552 kb on - strand, within BWI76_RS14235 at 2978.702 kb on - strand, within BWI76_RS14235 at 2978.744 kb on + strand, within BWI76_RS14235 at 2978.892 kb on + strand, within BWI76_RS14235 at 2978.900 kb on - strand, within BWI76_RS14235 at 2978.940 kb on + strand, within BWI76_RS14235 at 2979.367 kb on + strand at 2979.468 kb on - strand, within BWI76_RS14240 at 2979.563 kb on - strand, within BWI76_RS14240 at 2979.626 kb on + strand, within BWI76_RS14240 at 2979.634 kb on - strand, within BWI76_RS14240 at 2979.743 kb on + strand at 2979.796 kb on + strand, within BWI76_RS14245 at 2979.926 kb on + strand, within BWI76_RS14245 at 2979.943 kb on - strand, within BWI76_RS14245 at 2979.990 kb on - strand, within BWI76_RS14245 at 2980.261 kb on + strand at 2980.330 kb on - strand at 2980.581 kb on + strand at 2980.589 kb on - strand, within BWI76_RS14250
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,978,403 - +1.1 2,978,449 - BWI76_RS14235 0.14 +0.0 2,978,546 - BWI76_RS14235 0.24 +0.3 2,978,552 - BWI76_RS14235 0.24 +1.6 2,978,552 - BWI76_RS14235 0.24 -0.1 2,978,702 - BWI76_RS14235 0.39 +0.2 2,978,744 + BWI76_RS14235 0.43 +0.4 2,978,892 + BWI76_RS14235 0.57 -0.5 2,978,900 - BWI76_RS14235 0.58 -2.5 2,978,940 + BWI76_RS14235 0.62 -1.2 2,979,367 + +1.1 2,979,468 - BWI76_RS14240 0.30 -0.9 2,979,563 - BWI76_RS14240 0.59 -3.0 2,979,626 + BWI76_RS14240 0.79 +0.2 2,979,634 - BWI76_RS14240 0.81 -3.5 2,979,743 + -0.9 2,979,796 + BWI76_RS14245 0.13 -1.3 2,979,926 + BWI76_RS14245 0.38 +0.3 2,979,943 - BWI76_RS14245 0.41 -0.4 2,979,990 - BWI76_RS14245 0.50 -0.9 2,980,261 + -0.0 2,980,330 - -0.1 2,980,581 + -0.2 2,980,589 - BWI76_RS14250 0.16 -0.9
Or see this region's nucleotide sequence