Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14165 and BWI76_RS14170 are separated by 48 nucleotides BWI76_RS14170 and BWI76_RS14175 are separated by 135 nucleotides
BWI76_RS14165: BWI76_RS14165 - trans-acting regulatory protein of aco operon, at 2,963,756 to 2,966,509
_RS14165
BWI76_RS14170: BWI76_RS14170 - hypothetical protein, at 2,966,558 to 2,966,941
_RS14170
BWI76_RS14175: BWI76_RS14175 - dehydrogenase, at 2,967,077 to 2,967,952
_RS14175
Position (kb)
2966
2967 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2965.808 kb on + strand, within BWI76_RS14165 at 2965.813 kb on + strand, within BWI76_RS14165 at 2966.029 kb on + strand, within BWI76_RS14165 at 2966.029 kb on - strand, within BWI76_RS14165 at 2966.341 kb on + strand at 2966.408 kb on - strand at 2966.446 kb on + strand at 2966.561 kb on + strand at 2966.569 kb on - strand at 2966.579 kb on + strand at 2966.656 kb on + strand, within BWI76_RS14170 at 2966.741 kb on - strand, within BWI76_RS14170 at 2966.767 kb on - strand, within BWI76_RS14170 at 2966.840 kb on + strand, within BWI76_RS14170 at 2966.889 kb on - strand, within BWI76_RS14170 at 2966.907 kb on + strand at 2966.979 kb on + strand at 2966.979 kb on + strand at 2967.034 kb on - strand at 2967.036 kb on + strand at 2967.075 kb on + strand at 2967.075 kb on + strand at 2967.123 kb on + strand at 2967.147 kb on - strand at 2967.156 kb on + strand at 2967.220 kb on - strand, within BWI76_RS14175 at 2967.277 kb on + strand at 2967.285 kb on - strand, within BWI76_RS14175 at 2967.335 kb on - strand, within BWI76_RS14175 at 2967.571 kb on + strand, within BWI76_RS14175 at 2967.579 kb on - strand, within BWI76_RS14175 at 2967.708 kb on - strand, within BWI76_RS14175
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,965,808 + BWI76_RS14165 0.75 +0.8 2,965,813 + BWI76_RS14165 0.75 +0.2 2,966,029 + BWI76_RS14165 0.83 -0.1 2,966,029 - BWI76_RS14165 0.83 -0.4 2,966,341 + +0.5 2,966,408 - +1.1 2,966,446 + +0.1 2,966,561 + -1.4 2,966,569 - -0.1 2,966,579 + -0.3 2,966,656 + BWI76_RS14170 0.26 -2.0 2,966,741 - BWI76_RS14170 0.48 -2.5 2,966,767 - BWI76_RS14170 0.54 -0.2 2,966,840 + BWI76_RS14170 0.73 -0.2 2,966,889 - BWI76_RS14170 0.86 -0.4 2,966,907 + +0.4 2,966,979 + +0.5 2,966,979 + -2.5 2,967,034 - +0.5 2,967,036 + +0.1 2,967,075 + -2.0 2,967,075 + -1.1 2,967,123 + -1.8 2,967,147 - +0.6 2,967,156 + +2.4 2,967,220 - BWI76_RS14175 0.16 -0.4 2,967,277 + +4.3 2,967,285 - BWI76_RS14175 0.24 -1.6 2,967,335 - BWI76_RS14175 0.29 -0.8 2,967,571 + BWI76_RS14175 0.56 -0.3 2,967,579 - BWI76_RS14175 0.57 +0.5 2,967,708 - BWI76_RS14175 0.72 +0.3
Or see this region's nucleotide sequence