Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13535 and BWI76_RS13540 are separated by 15 nucleotides BWI76_RS13540 and BWI76_RS13545 are separated by 11 nucleotides BWI76_RS13545 and BWI76_RS13550 are separated by 9 nucleotides BWI76_RS13550 and BWI76_RS13555 overlap by 17 nucleotides
BWI76_RS13535: BWI76_RS13535 - LLM class flavin-dependent oxidoreductase, at 2,822,196 to 2,823,512
_RS13535
BWI76_RS13540: BWI76_RS13540 - ABC transporter substrate-binding protein, at 2,823,528 to 2,824,469
_RS13540
BWI76_RS13545: BWI76_RS13545 - N-acetyltransferase, at 2,824,481 to 2,824,990
_RS13545
BWI76_RS13550: BWI76_RS13550 - polar amino acid ABC transporter inner membrane subunit, at 2,825,000 to 2,825,926
_RS13550
BWI76_RS13555: BWI76_RS13555 - ABC transporter, at 2,825,910 to 2,826,689
_RS13555
Position (kb)
2824
2825 Strain fitness (log2 ratio)
-1
0
1 at 2823.515 kb on - strand at 2823.781 kb on + strand, within BWI76_RS13540 at 2823.832 kb on + strand, within BWI76_RS13540 at 2823.881 kb on + strand, within BWI76_RS13540 at 2824.032 kb on + strand, within BWI76_RS13540 at 2824.100 kb on - strand, within BWI76_RS13540 at 2824.167 kb on + strand, within BWI76_RS13540 at 2824.175 kb on - strand, within BWI76_RS13540 at 2824.910 kb on + strand, within BWI76_RS13545 at 2825.210 kb on + strand, within BWI76_RS13550 at 2825.514 kb on - strand, within BWI76_RS13550 at 2825.514 kb on - strand, within BWI76_RS13550 at 2825.538 kb on - strand, within BWI76_RS13550 at 2825.652 kb on - strand, within BWI76_RS13550 at 2825.689 kb on + strand, within BWI76_RS13550 at 2825.697 kb on - strand, within BWI76_RS13550 at 2825.716 kb on - strand, within BWI76_RS13550 at 2825.791 kb on + strand, within BWI76_RS13550 at 2825.902 kb on - strand at 2825.906 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,823,515 - +0.0 2,823,781 + BWI76_RS13540 0.27 +0.2 2,823,832 + BWI76_RS13540 0.32 +0.6 2,823,881 + BWI76_RS13540 0.37 -0.5 2,824,032 + BWI76_RS13540 0.54 +1.2 2,824,100 - BWI76_RS13540 0.61 -0.2 2,824,167 + BWI76_RS13540 0.68 -0.1 2,824,175 - BWI76_RS13540 0.69 +0.3 2,824,910 + BWI76_RS13545 0.84 -1.0 2,825,210 + BWI76_RS13550 0.23 +0.7 2,825,514 - BWI76_RS13550 0.55 +0.2 2,825,514 - BWI76_RS13550 0.55 +0.3 2,825,538 - BWI76_RS13550 0.58 -0.0 2,825,652 - BWI76_RS13550 0.70 +0.3 2,825,689 + BWI76_RS13550 0.74 +0.4 2,825,697 - BWI76_RS13550 0.75 +0.4 2,825,716 - BWI76_RS13550 0.77 +0.0 2,825,791 + BWI76_RS13550 0.85 +0.3 2,825,902 - +0.6 2,825,906 + +0.5
Or see this region's nucleotide sequence