Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12210 and BWI76_RS12215 are separated by 61 nucleotides BWI76_RS12215 and BWI76_RS12220 are separated by 389 nucleotides BWI76_RS12220 and BWI76_RS12225 are separated by 364 nucleotides
BWI76_RS12210: BWI76_RS12210 - hypothetical protein, at 2,554,944 to 2,555,189
_RS12210
BWI76_RS12215: BWI76_RS12215 - HNH endonuclease, at 2,555,251 to 2,555,376
_RS12215
BWI76_RS12220: BWI76_RS12220 - hypothetical protein, at 2,555,766 to 2,556,005
_RS12220
BWI76_RS12225: BWI76_RS12225 - hypothetical protein, at 2,556,370 to 2,556,543
_RS12225
Position (kb)
2555
2556
2557 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2554.798 kb on + strand at 2554.860 kb on - strand at 2554.865 kb on - strand at 2554.894 kb on + strand at 2554.929 kb on + strand at 2554.936 kb on + strand at 2554.936 kb on + strand at 2554.978 kb on - strand, within BWI76_RS12210 at 2554.985 kb on + strand, within BWI76_RS12210 at 2554.992 kb on + strand, within BWI76_RS12210 at 2555.000 kb on - strand, within BWI76_RS12210 at 2555.000 kb on - strand, within BWI76_RS12210 at 2555.000 kb on - strand, within BWI76_RS12210 at 2555.037 kb on + strand, within BWI76_RS12210 at 2555.045 kb on - strand at 2555.076 kb on + strand, within BWI76_RS12210 at 2555.087 kb on + strand, within BWI76_RS12210 at 2555.139 kb on - strand, within BWI76_RS12210 at 2555.159 kb on + strand, within BWI76_RS12210 at 2555.167 kb on - strand at 2555.170 kb on + strand at 2555.170 kb on + strand at 2555.178 kb on - strand at 2555.178 kb on - strand at 2555.178 kb on - strand at 2555.192 kb on - strand at 2555.242 kb on + strand at 2555.270 kb on - strand, within BWI76_RS12215 at 2555.312 kb on + strand, within BWI76_RS12215 at 2555.312 kb on + strand, within BWI76_RS12215 at 2555.320 kb on - strand, within BWI76_RS12215 at 2555.329 kb on - strand, within BWI76_RS12215 at 2555.342 kb on - strand, within BWI76_RS12215 at 2555.353 kb on - strand, within BWI76_RS12215 at 2555.354 kb on + strand, within BWI76_RS12215 at 2555.362 kb on - strand, within BWI76_RS12215 at 2555.372 kb on - strand at 2555.381 kb on + strand at 2555.387 kb on - strand at 2555.416 kb on - strand at 2555.421 kb on - strand at 2555.446 kb on - strand at 2555.470 kb on - strand at 2555.511 kb on - strand at 2555.548 kb on + strand at 2555.548 kb on + strand at 2555.554 kb on - strand at 2555.556 kb on - strand at 2555.561 kb on + strand at 2555.591 kb on + strand at 2555.612 kb on - strand at 2555.628 kb on + strand at 2555.641 kb on + strand at 2555.652 kb on + strand at 2555.694 kb on + strand at 2555.694 kb on + strand at 2555.797 kb on + strand, within BWI76_RS12220 at 2555.808 kb on - strand, within BWI76_RS12220 at 2555.814 kb on + strand, within BWI76_RS12220 at 2555.814 kb on + strand, within BWI76_RS12220 at 2555.817 kb on - strand, within BWI76_RS12220 at 2555.817 kb on - strand, within BWI76_RS12220 at 2555.819 kb on + strand, within BWI76_RS12220 at 2555.822 kb on - strand, within BWI76_RS12220 at 2555.840 kb on + strand, within BWI76_RS12220 at 2555.848 kb on - strand, within BWI76_RS12220 at 2555.848 kb on - strand, within BWI76_RS12220 at 2555.884 kb on + strand, within BWI76_RS12220 at 2555.884 kb on + strand, within BWI76_RS12220 at 2555.892 kb on - strand, within BWI76_RS12220 at 2555.892 kb on - strand, within BWI76_RS12220 at 2555.892 kb on - strand, within BWI76_RS12220 at 2555.908 kb on - strand, within BWI76_RS12220 at 2555.951 kb on + strand, within BWI76_RS12220 at 2555.951 kb on + strand, within BWI76_RS12220 at 2555.951 kb on + strand, within BWI76_RS12220 at 2555.951 kb on + strand, within BWI76_RS12220 at 2555.961 kb on - strand, within BWI76_RS12220 at 2556.091 kb on + strand at 2556.091 kb on + strand at 2556.091 kb on + strand at 2556.091 kb on + strand at 2556.091 kb on + strand at 2556.091 kb on + strand at 2556.099 kb on + strand at 2556.099 kb on - strand at 2556.100 kb on + strand at 2556.101 kb on + strand at 2556.101 kb on + strand at 2556.105 kb on + strand at 2556.114 kb on + strand at 2556.114 kb on + strand at 2556.114 kb on + strand at 2556.122 kb on - strand at 2556.137 kb on + strand at 2556.140 kb on - strand at 2556.191 kb on + strand at 2556.195 kb on + strand at 2556.240 kb on + strand at 2556.245 kb on + strand at 2556.250 kb on + strand at 2556.250 kb on + strand at 2556.250 kb on + strand at 2556.250 kb on + strand at 2556.250 kb on + strand at 2556.250 kb on + strand at 2556.250 kb on + strand at 2556.250 kb on + strand at 2556.250 kb on + strand at 2556.250 kb on + strand at 2556.258 kb on - strand at 2556.258 kb on - strand at 2556.258 kb on - strand at 2556.258 kb on - strand at 2556.258 kb on - strand at 2556.258 kb on - strand at 2556.258 kb on - strand at 2556.258 kb on - strand at 2556.260 kb on + strand at 2556.290 kb on - strand at 2556.299 kb on - strand at 2556.301 kb on + strand at 2556.301 kb on + strand at 2556.354 kb on + strand at 2556.354 kb on + strand at 2556.354 kb on + strand at 2556.354 kb on + strand at 2556.360 kb on - strand at 2556.360 kb on - strand at 2556.362 kb on - strand at 2556.362 kb on - strand at 2556.362 kb on - strand at 2556.362 kb on - strand at 2556.362 kb on - strand at 2556.362 kb on - strand at 2556.362 kb on - strand at 2556.362 kb on - strand at 2556.506 kb on + strand, within BWI76_RS12225 at 2556.506 kb on + strand, within BWI76_RS12225 at 2556.576 kb on + strand at 2556.576 kb on + strand at 2556.579 kb on + strand at 2556.579 kb on + strand at 2556.589 kb on + strand at 2556.589 kb on + strand at 2556.591 kb on - strand at 2556.591 kb on - strand at 2556.592 kb on - strand at 2556.592 kb on - strand at 2556.601 kb on - strand at 2556.621 kb on - strand at 2556.636 kb on + strand at 2556.636 kb on + strand at 2556.658 kb on - strand at 2556.662 kb on + strand at 2556.663 kb on - strand at 2556.686 kb on - strand at 2556.767 kb on + strand at 2556.806 kb on + strand at 2556.808 kb on + strand at 2556.808 kb on + strand at 2556.808 kb on + strand at 2556.808 kb on + strand at 2556.816 kb on - strand at 2556.816 kb on - strand at 2556.840 kb on + strand at 2556.841 kb on + strand at 2556.842 kb on + strand at 2556.843 kb on + strand at 2556.843 kb on - strand at 2556.848 kb on - strand at 2556.867 kb on - strand at 2556.867 kb on - strand at 2556.868 kb on - strand at 2556.870 kb on + strand at 2556.870 kb on + strand at 2556.870 kb on + strand at 2556.870 kb on + strand at 2556.870 kb on + strand at 2556.870 kb on + strand at 2556.878 kb on - strand at 2556.878 kb on - strand at 2556.878 kb on - strand at 2556.878 kb on - strand at 2556.878 kb on - strand at 2556.879 kb on - strand at 2556.886 kb on - strand at 2556.889 kb on - strand at 2556.900 kb on - strand at 2556.907 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,554,798 + -0.4 2,554,860 - -2.3 2,554,865 - -1.1 2,554,894 + -1.5 2,554,929 + -1.6 2,554,936 + -0.1 2,554,936 + -1.3 2,554,978 - BWI76_RS12210 0.14 -0.6 2,554,985 + BWI76_RS12210 0.17 -0.2 2,554,992 + BWI76_RS12210 0.20 -0.3 2,555,000 - BWI76_RS12210 0.23 +0.1 2,555,000 - BWI76_RS12210 0.23 -0.1 2,555,000 - BWI76_RS12210 0.23 -0.9 2,555,037 + BWI76_RS12210 0.38 -1.0 2,555,045 - -0.0 2,555,076 + BWI76_RS12210 0.54 -0.6 2,555,087 + BWI76_RS12210 0.58 -0.7 2,555,139 - BWI76_RS12210 0.79 +0.3 2,555,159 + BWI76_RS12210 0.87 -0.7 2,555,167 - -0.7 2,555,170 + -2.2 2,555,170 + -1.5 2,555,178 - -2.2 2,555,178 - -1.0 2,555,178 - -0.9 2,555,192 - -0.1 2,555,242 + -0.6 2,555,270 - BWI76_RS12215 0.15 -1.2 2,555,312 + BWI76_RS12215 0.48 -2.4 2,555,312 + BWI76_RS12215 0.48 -1.5 2,555,320 - BWI76_RS12215 0.55 -1.9 2,555,329 - BWI76_RS12215 0.62 -1.2 2,555,342 - BWI76_RS12215 0.72 -0.7 2,555,353 - BWI76_RS12215 0.81 -0.8 2,555,354 + BWI76_RS12215 0.82 -0.3 2,555,362 - BWI76_RS12215 0.88 -3.7 2,555,372 - -1.0 2,555,381 + +3.3 2,555,387 - -0.4 2,555,416 - -1.1 2,555,421 - -2.2 2,555,446 - -0.6 2,555,470 - -2.7 2,555,511 - -2.2 2,555,548 + +0.4 2,555,548 + -0.6 2,555,554 - +0.4 2,555,556 - -0.2 2,555,561 + -0.5 2,555,591 + +1.6 2,555,612 - -0.3 2,555,628 + -1.8 2,555,641 + -0.8 2,555,652 + -1.0 2,555,694 + -0.6 2,555,694 + -1.5 2,555,797 + BWI76_RS12220 0.13 -1.1 2,555,808 - BWI76_RS12220 0.17 -1.8 2,555,814 + BWI76_RS12220 0.20 -3.4 2,555,814 + BWI76_RS12220 0.20 -2.0 2,555,817 - BWI76_RS12220 0.21 -2.1 2,555,817 - BWI76_RS12220 0.21 -2.2 2,555,819 + BWI76_RS12220 0.22 -0.7 2,555,822 - BWI76_RS12220 0.23 -0.4 2,555,840 + BWI76_RS12220 0.31 +1.2 2,555,848 - BWI76_RS12220 0.34 -1.1 2,555,848 - BWI76_RS12220 0.34 -3.8 2,555,884 + BWI76_RS12220 0.49 -0.7 2,555,884 + BWI76_RS12220 0.49 +1.0 2,555,892 - BWI76_RS12220 0.53 -0.6 2,555,892 - BWI76_RS12220 0.53 -1.3 2,555,892 - BWI76_RS12220 0.53 -0.2 2,555,908 - BWI76_RS12220 0.59 +1.0 2,555,951 + BWI76_RS12220 0.77 -0.8 2,555,951 + BWI76_RS12220 0.77 +0.2 2,555,951 + BWI76_RS12220 0.77 -1.2 2,555,951 + BWI76_RS12220 0.77 -1.5 2,555,961 - BWI76_RS12220 0.81 -1.6 2,556,091 + -0.3 2,556,091 + -0.5 2,556,091 + -2.0 2,556,091 + -0.6 2,556,091 + -0.0 2,556,091 + -0.1 2,556,099 + +0.5 2,556,099 - -0.3 2,556,100 + +1.3 2,556,101 + -0.7 2,556,101 + -0.5 2,556,105 + -1.3 2,556,114 + -0.7 2,556,114 + -0.2 2,556,114 + -2.9 2,556,122 - -1.3 2,556,137 + -1.8 2,556,140 - +1.0 2,556,191 + +0.1 2,556,195 + +0.2 2,556,240 + -0.5 2,556,245 + -0.3 2,556,250 + -0.5 2,556,250 + -1.1 2,556,250 + -0.8 2,556,250 + -0.9 2,556,250 + -0.4 2,556,250 + +0.7 2,556,250 + -0.2 2,556,250 + -2.7 2,556,250 + -1.0 2,556,250 + -1.4 2,556,258 - -1.1 2,556,258 - -0.7 2,556,258 - +0.9 2,556,258 - +0.5 2,556,258 - -2.0 2,556,258 - -0.5 2,556,258 - -0.2 2,556,258 - +1.0 2,556,260 + -0.0 2,556,290 - -1.1 2,556,299 - -1.4 2,556,301 + -2.8 2,556,301 + -1.0 2,556,354 + +0.1 2,556,354 + +0.8 2,556,354 + -0.6 2,556,354 + -0.0 2,556,360 - -0.4 2,556,360 - +0.2 2,556,362 - -0.8 2,556,362 - -3.5 2,556,362 - -1.2 2,556,362 - +0.2 2,556,362 - -1.0 2,556,362 - -1.8 2,556,362 - -0.6 2,556,362 - -3.3 2,556,506 + BWI76_RS12225 0.78 -0.6 2,556,506 + BWI76_RS12225 0.78 -1.6 2,556,576 + -2.3 2,556,576 + -0.5 2,556,579 + -2.0 2,556,579 + -0.6 2,556,589 + -0.6 2,556,589 + -0.6 2,556,591 - -0.9 2,556,591 - -1.3 2,556,592 - -0.7 2,556,592 - -1.6 2,556,601 - -0.7 2,556,621 - -1.6 2,556,636 + -0.5 2,556,636 + -1.0 2,556,658 - -0.6 2,556,662 + -0.2 2,556,663 - -0.1 2,556,686 - -0.6 2,556,767 + +0.6 2,556,806 + +0.1 2,556,808 + -2.0 2,556,808 + -0.6 2,556,808 + -0.4 2,556,808 + -0.9 2,556,816 - -3.3 2,556,816 - -0.9 2,556,840 + +0.2 2,556,841 + -0.8 2,556,842 + -2.1 2,556,843 + -0.8 2,556,843 - -1.2 2,556,848 - -2.9 2,556,867 - +0.0 2,556,867 - +0.4 2,556,868 - -0.4 2,556,870 + -0.4 2,556,870 + -1.0 2,556,870 + +0.8 2,556,870 + -0.3 2,556,870 + -1.3 2,556,870 + -0.1 2,556,878 - -0.3 2,556,878 - -0.2 2,556,878 - -2.0 2,556,878 - -0.9 2,556,878 - -0.6 2,556,879 - -1.7 2,556,886 - +0.3 2,556,889 - -1.2 2,556,900 - -0.9 2,556,907 + -1.6
Or see this region's nucleotide sequence