Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12095 and BWI76_RS12100 overlap by 4 nucleotides BWI76_RS12100 and BWI76_RS12105 overlap by 8 nucleotides BWI76_RS12105 and BWI76_RS12110 overlap by 8 nucleotides BWI76_RS12110 and BWI76_RS12115 overlap by 4 nucleotides BWI76_RS12115 and BWI76_RS12120 overlap by 8 nucleotides
BWI76_RS12095: BWI76_RS12095 - hypothetical protein, at 2,540,164 to 2,541,084
_RS12095
BWI76_RS12100: BWI76_RS12100 - replication protein P, at 2,541,081 to 2,541,824
_RS12100
BWI76_RS12105: BWI76_RS12105 - hypothetical protein, at 2,541,817 to 2,542,152
_RS12105
BWI76_RS12110: BWI76_RS12110 - hypothetical protein, at 2,542,145 to 2,542,930
_RS12110
BWI76_RS12115: BWI76_RS12115 - hypothetical protein, at 2,542,927 to 2,543,130
_RS12115
BWI76_RS12120: BWI76_RS12120 - hypothetical protein, at 2,543,123 to 2,543,308
_RS12120
Position (kb)
2541
2542
2543 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2540.842 kb on - strand, within BWI76_RS12095 at 2541.008 kb on + strand at 2541.008 kb on + strand at 2541.016 kb on - strand at 2541.104 kb on + strand at 2541.133 kb on - strand at 2541.426 kb on - strand, within BWI76_RS12100 at 2541.524 kb on + strand, within BWI76_RS12100 at 2541.532 kb on - strand, within BWI76_RS12100 at 2541.618 kb on + strand, within BWI76_RS12100 at 2541.626 kb on - strand, within BWI76_RS12100 at 2541.636 kb on - strand, within BWI76_RS12100 at 2541.709 kb on + strand, within BWI76_RS12100 at 2542.011 kb on - strand, within BWI76_RS12105 at 2542.050 kb on + strand, within BWI76_RS12105 at 2542.289 kb on + strand, within BWI76_RS12110 at 2542.297 kb on - strand, within BWI76_RS12110 at 2542.297 kb on - strand at 2542.297 kb on - strand, within BWI76_RS12110 at 2542.297 kb on - strand, within BWI76_RS12110 at 2542.309 kb on + strand, within BWI76_RS12110 at 2542.383 kb on + strand, within BWI76_RS12110 at 2542.613 kb on + strand, within BWI76_RS12110 at 2542.655 kb on - strand, within BWI76_RS12110 at 2542.697 kb on + strand, within BWI76_RS12110 at 2542.866 kb on + strand at 2543.010 kb on - strand, within BWI76_RS12115 at 2543.010 kb on - strand, within BWI76_RS12115 at 2543.015 kb on - strand, within BWI76_RS12115 at 2543.015 kb on - strand, within BWI76_RS12115 at 2543.107 kb on - strand, within BWI76_RS12115 at 2543.108 kb on - strand, within BWI76_RS12115
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,540,842 - BWI76_RS12095 0.74 +0.6 2,541,008 + +0.5 2,541,008 + +1.0 2,541,016 - -0.3 2,541,104 + +0.0 2,541,133 - +0.2 2,541,426 - BWI76_RS12100 0.46 +0.4 2,541,524 + BWI76_RS12100 0.60 +0.4 2,541,532 - BWI76_RS12100 0.61 -0.2 2,541,618 + BWI76_RS12100 0.72 +2.3 2,541,626 - BWI76_RS12100 0.73 +0.0 2,541,636 - BWI76_RS12100 0.75 -2.7 2,541,709 + BWI76_RS12100 0.84 +0.3 2,542,011 - BWI76_RS12105 0.58 -0.8 2,542,050 + BWI76_RS12105 0.69 -2.0 2,542,289 + BWI76_RS12110 0.18 -0.1 2,542,297 - BWI76_RS12110 0.19 -0.9 2,542,297 - -1.3 2,542,297 - BWI76_RS12110 0.19 -1.0 2,542,297 - BWI76_RS12110 0.19 -0.6 2,542,309 + BWI76_RS12110 0.21 +0.6 2,542,383 + BWI76_RS12110 0.30 -0.3 2,542,613 + BWI76_RS12110 0.60 -1.0 2,542,655 - BWI76_RS12110 0.65 +0.6 2,542,697 + BWI76_RS12110 0.70 +0.4 2,542,866 + -0.5 2,543,010 - BWI76_RS12115 0.41 -0.7 2,543,010 - BWI76_RS12115 0.41 -0.5 2,543,015 - BWI76_RS12115 0.43 -0.1 2,543,015 - BWI76_RS12115 0.43 -0.7 2,543,107 - BWI76_RS12115 0.88 -0.9 2,543,108 - BWI76_RS12115 0.89 -1.3
Or see this region's nucleotide sequence