Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09820

Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09810 and BWI76_RS09815 overlap by 4 nucleotidesBWI76_RS09815 and BWI76_RS09820 are separated by 141 nucleotidesBWI76_RS09820 and BWI76_RS09825 overlap by 24 nucleotidesBWI76_RS09825 and BWI76_RS09830 are separated by 121 nucleotides BWI76_RS09810: BWI76_RS09810 - hypothetical protein, at 2,059,189 to 2,059,371 _RS09810 BWI76_RS09815: BWI76_RS09815 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 2,059,368 to 2,060,114 _RS09815 BWI76_RS09820: BWI76_RS09820 - hypothetical protein, at 2,060,256 to 2,061,149 _RS09820 BWI76_RS09825: BWI76_RS09825 - envelope biogenesis factor ElyC, at 2,061,126 to 2,061,905 _RS09825 BWI76_RS09830: BWI76_RS09830 - tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM, at 2,062,027 to 2,062,806 _RS09830 Position (kb) 2060 2061 2062Strain fitness (log2 ratio) -2 -1 0 1at 2060.371 kb on + strand, within BWI76_RS09820at 2060.689 kb on - strand, within BWI76_RS09820at 2060.851 kb on + strand, within BWI76_RS09820at 2060.870 kb on + strand, within BWI76_RS09820at 2060.915 kb on + strand, within BWI76_RS09820at 2061.255 kb on - strand, within BWI76_RS09825at 2061.333 kb on + strand, within BWI76_RS09825at 2061.806 kb on + strand, within BWI76_RS09825at 2061.811 kb on + strand, within BWI76_RS09825at 2061.814 kb on - strand, within BWI76_RS09825at 2061.990 kb on - strandat 2062.032 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,060,371 + BWI76_RS09820 0.13 +0.8
2,060,689 - BWI76_RS09820 0.48 +0.8
2,060,851 + BWI76_RS09820 0.67 +0.2
2,060,870 + BWI76_RS09820 0.69 +0.1
2,060,915 + BWI76_RS09820 0.74 -0.4
2,061,255 - BWI76_RS09825 0.17 -1.8
2,061,333 + BWI76_RS09825 0.27 -1.6
2,061,806 + BWI76_RS09825 0.87 -0.8
2,061,811 + BWI76_RS09825 0.88 -1.4
2,061,814 - BWI76_RS09825 0.88 -2.6
2,061,990 - +0.8
2,062,032 - +0.2

Or see this region's nucleotide sequence