Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06975

Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06970 and BWI76_RS06975 are separated by 87 nucleotidesBWI76_RS06975 and BWI76_RS06980 are separated by 210 nucleotidesBWI76_RS06980 and BWI76_RS06985 are separated by 91 nucleotides BWI76_RS06970: BWI76_RS06970 - selenophosphate-dependent tRNA 2-selenouridine synthase, at 1,463,888 to 1,464,973 _RS06970 BWI76_RS06975: BWI76_RS06975 - DNA-binding transcriptional activator AllS, at 1,465,061 to 1,465,984 _RS06975 BWI76_RS06980: BWI76_RS06980 - ureidoglycolate lyase, at 1,466,195 to 1,466,677 _RS06980 BWI76_RS06985: BWI76_RS06985 - transcriptional regulator, at 1,466,769 to 1,467,587 _RS06985 Position (kb) 1465 1466Strain fitness (log2 ratio) -2 -1 0 1at 1464.424 kb on + strand, within BWI76_RS06970at 1464.550 kb on - strand, within BWI76_RS06970at 1464.601 kb on + strand, within BWI76_RS06970at 1464.622 kb on - strand, within BWI76_RS06970at 1464.637 kb on - strand, within BWI76_RS06970at 1464.846 kb on + strand, within BWI76_RS06970at 1464.925 kb on + strandat 1464.982 kb on + strandat 1465.048 kb on - strandat 1465.074 kb on - strandat 1465.141 kb on - strandat 1465.143 kb on + strandat 1465.208 kb on - strand, within BWI76_RS06975at 1465.347 kb on + strand, within BWI76_RS06975at 1465.372 kb on + strand, within BWI76_RS06975at 1465.542 kb on - strand, within BWI76_RS06975at 1465.646 kb on + strand, within BWI76_RS06975at 1465.925 kb on + strandat 1465.928 kb on - strandat 1466.133 kb on + strandat 1466.152 kb on + strandat 1466.153 kb on - strandat 1466.499 kb on + strand, within BWI76_RS06980at 1466.835 kb on + strandat 1466.978 kb on - strand, within BWI76_RS06985

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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1,464,424 + BWI76_RS06970 0.49 +0.7
1,464,550 - BWI76_RS06970 0.61 -2.6
1,464,601 + BWI76_RS06970 0.66 +0.3
1,464,622 - BWI76_RS06970 0.68 +0.9
1,464,637 - BWI76_RS06970 0.69 +0.4
1,464,846 + BWI76_RS06970 0.88 +1.4
1,464,925 + -0.5
1,464,982 + -0.2
1,465,048 - +0.5
1,465,074 - -0.0
1,465,141 - +0.6
1,465,143 + -0.1
1,465,208 - BWI76_RS06975 0.16 +0.5
1,465,347 + BWI76_RS06975 0.31 -0.2
1,465,372 + BWI76_RS06975 0.34 -0.1
1,465,542 - BWI76_RS06975 0.52 +0.5
1,465,646 + BWI76_RS06975 0.63 +0.9
1,465,925 + +1.1
1,465,928 - +0.0
1,466,133 + +0.2
1,466,152 + -0.3
1,466,153 - -0.5
1,466,499 + BWI76_RS06980 0.63 +0.7
1,466,835 + +1.2
1,466,978 - BWI76_RS06985 0.26 -0.2

Or see this region's nucleotide sequence