Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06970 and BWI76_RS06975 are separated by 87 nucleotides BWI76_RS06975 and BWI76_RS06980 are separated by 210 nucleotides BWI76_RS06980 and BWI76_RS06985 are separated by 91 nucleotides
BWI76_RS06970: BWI76_RS06970 - selenophosphate-dependent tRNA 2-selenouridine synthase, at 1,463,888 to 1,464,973
_RS06970
BWI76_RS06975: BWI76_RS06975 - DNA-binding transcriptional activator AllS, at 1,465,061 to 1,465,984
_RS06975
BWI76_RS06980: BWI76_RS06980 - ureidoglycolate lyase, at 1,466,195 to 1,466,677
_RS06980
BWI76_RS06985: BWI76_RS06985 - transcriptional regulator, at 1,466,769 to 1,467,587
_RS06985
Position (kb)
1465
1466 Strain fitness (log2 ratio)
-2
-1
0
1 at 1464.424 kb on + strand, within BWI76_RS06970 at 1464.550 kb on - strand, within BWI76_RS06970 at 1464.601 kb on + strand, within BWI76_RS06970 at 1464.622 kb on - strand, within BWI76_RS06970 at 1464.637 kb on - strand, within BWI76_RS06970 at 1464.846 kb on + strand, within BWI76_RS06970 at 1464.925 kb on + strand at 1464.982 kb on + strand at 1465.048 kb on - strand at 1465.074 kb on - strand at 1465.141 kb on - strand at 1465.143 kb on + strand at 1465.208 kb on - strand, within BWI76_RS06975 at 1465.347 kb on + strand, within BWI76_RS06975 at 1465.372 kb on + strand, within BWI76_RS06975 at 1465.542 kb on - strand, within BWI76_RS06975 at 1465.646 kb on + strand, within BWI76_RS06975 at 1465.925 kb on + strand at 1465.928 kb on - strand at 1466.133 kb on + strand at 1466.152 kb on + strand at 1466.153 kb on - strand at 1466.499 kb on + strand, within BWI76_RS06980 at 1466.835 kb on + strand at 1466.978 kb on - strand, within BWI76_RS06985
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,464,424 + BWI76_RS06970 0.49 +0.7 1,464,550 - BWI76_RS06970 0.61 -2.6 1,464,601 + BWI76_RS06970 0.66 +0.3 1,464,622 - BWI76_RS06970 0.68 +0.9 1,464,637 - BWI76_RS06970 0.69 +0.4 1,464,846 + BWI76_RS06970 0.88 +1.4 1,464,925 + -0.5 1,464,982 + -0.2 1,465,048 - +0.5 1,465,074 - -0.0 1,465,141 - +0.6 1,465,143 + -0.1 1,465,208 - BWI76_RS06975 0.16 +0.5 1,465,347 + BWI76_RS06975 0.31 -0.2 1,465,372 + BWI76_RS06975 0.34 -0.1 1,465,542 - BWI76_RS06975 0.52 +0.5 1,465,646 + BWI76_RS06975 0.63 +0.9 1,465,925 + +1.1 1,465,928 - +0.0 1,466,133 + +0.2 1,466,152 + -0.3 1,466,153 - -0.5 1,466,499 + BWI76_RS06980 0.63 +0.7 1,466,835 + +1.2 1,466,978 - BWI76_RS06985 0.26 -0.2
Or see this region's nucleotide sequence