Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06735 and BWI76_RS06740 overlap by 1 nucleotides BWI76_RS06740 and BWI76_RS06745 overlap by 4 nucleotides BWI76_RS06745 and BWI76_RS06750 are separated by 100 nucleotides BWI76_RS06750 and BWI76_RS06755 overlap by 26 nucleotides BWI76_RS06755 and BWI76_RS06760 are separated by 126 nucleotides
BWI76_RS06735: BWI76_RS06735 - 50S ribosomal protein L31, at 1,413,802 to 1,414,065
_RS06735
BWI76_RS06740: BWI76_RS06740 - 50S ribosomal protein L36, at 1,414,065 to 1,414,205
_RS06740
BWI76_RS06745: BWI76_RS06745 - N-acetyltransferase GCN5, at 1,414,202 to 1,414,900
_RS06745
BWI76_RS06750: BWI76_RS06750 - GntR family transcriptional regulator with, at 1,415,001 to 1,416,455
_RS06750
BWI76_RS06755: BWI76_RS06755 - sigma factor YlaC, at 1,416,430 to 1,416,900
_RS06755
BWI76_RS06760: BWI76_RS06760 - maltose O-acetyltransferase, at 1,417,027 to 1,417,593
_RS06760
Position (kb)
1415
1416
1417 Strain fitness (log2 ratio)
-2
-1
0
1 at 1414.080 kb on + strand, within BWI76_RS06740 at 1414.090 kb on + strand, within BWI76_RS06740 at 1414.169 kb on + strand, within BWI76_RS06740 at 1414.533 kb on + strand, within BWI76_RS06745 at 1414.656 kb on + strand, within BWI76_RS06745 at 1414.752 kb on + strand at 1414.762 kb on + strand, within BWI76_RS06745 at 1414.893 kb on - strand at 1414.903 kb on - strand at 1415.175 kb on - strand, within BWI76_RS06750 at 1415.187 kb on + strand, within BWI76_RS06750 at 1415.187 kb on + strand, within BWI76_RS06750 at 1415.247 kb on + strand, within BWI76_RS06750 at 1415.264 kb on + strand, within BWI76_RS06750 at 1415.374 kb on - strand, within BWI76_RS06750 at 1415.424 kb on - strand, within BWI76_RS06750 at 1415.536 kb on - strand, within BWI76_RS06750 at 1415.548 kb on + strand, within BWI76_RS06750 at 1415.604 kb on + strand, within BWI76_RS06750 at 1415.612 kb on - strand, within BWI76_RS06750 at 1415.737 kb on + strand, within BWI76_RS06750 at 1415.802 kb on + strand, within BWI76_RS06750 at 1415.877 kb on + strand, within BWI76_RS06750 at 1415.885 kb on - strand, within BWI76_RS06750 at 1415.916 kb on - strand, within BWI76_RS06750 at 1416.071 kb on + strand, within BWI76_RS06750 at 1416.366 kb on + strand at 1416.366 kb on + strand at 1416.668 kb on - strand, within BWI76_RS06755 at 1416.747 kb on + strand, within BWI76_RS06755 at 1416.755 kb on - strand at 1416.755 kb on - strand, within BWI76_RS06755 at 1416.767 kb on + strand, within BWI76_RS06755 at 1417.026 kb on - strand at 1417.093 kb on - strand, within BWI76_RS06760 at 1417.165 kb on - strand, within BWI76_RS06760 at 1417.196 kb on - strand, within BWI76_RS06760 at 1417.227 kb on - strand, within BWI76_RS06760 at 1417.290 kb on + strand, within BWI76_RS06760 at 1417.297 kb on - strand, within BWI76_RS06760 at 1417.408 kb on + strand, within BWI76_RS06760
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,414,080 + BWI76_RS06740 0.11 -0.4 1,414,090 + BWI76_RS06740 0.18 +0.3 1,414,169 + BWI76_RS06740 0.74 +0.9 1,414,533 + BWI76_RS06745 0.47 -1.5 1,414,656 + BWI76_RS06745 0.65 +0.2 1,414,752 + +0.4 1,414,762 + BWI76_RS06745 0.80 +0.4 1,414,893 - -0.2 1,414,903 - +0.8 1,415,175 - BWI76_RS06750 0.12 +0.8 1,415,187 + BWI76_RS06750 0.13 -0.2 1,415,187 + BWI76_RS06750 0.13 +0.5 1,415,247 + BWI76_RS06750 0.17 -0.2 1,415,264 + BWI76_RS06750 0.18 +0.6 1,415,374 - BWI76_RS06750 0.26 +0.2 1,415,424 - BWI76_RS06750 0.29 +0.8 1,415,536 - BWI76_RS06750 0.37 +1.3 1,415,548 + BWI76_RS06750 0.38 +0.3 1,415,604 + BWI76_RS06750 0.41 +0.2 1,415,612 - BWI76_RS06750 0.42 -1.7 1,415,737 + BWI76_RS06750 0.51 +0.1 1,415,802 + BWI76_RS06750 0.55 +0.0 1,415,877 + BWI76_RS06750 0.60 +1.2 1,415,885 - BWI76_RS06750 0.61 +1.6 1,415,916 - BWI76_RS06750 0.63 +0.2 1,416,071 + BWI76_RS06750 0.74 +0.5 1,416,366 + +0.3 1,416,366 + -1.2 1,416,668 - BWI76_RS06755 0.51 +0.5 1,416,747 + BWI76_RS06755 0.67 +0.7 1,416,755 - +0.6 1,416,755 - BWI76_RS06755 0.69 -0.7 1,416,767 + BWI76_RS06755 0.72 +0.3 1,417,026 - +0.7 1,417,093 - BWI76_RS06760 0.12 +0.2 1,417,165 - BWI76_RS06760 0.24 -2.6 1,417,196 - BWI76_RS06760 0.30 +0.4 1,417,227 - BWI76_RS06760 0.35 +0.2 1,417,290 + BWI76_RS06760 0.46 -1.5 1,417,297 - BWI76_RS06760 0.48 -0.1 1,417,408 + BWI76_RS06760 0.67 +0.1
Or see this region's nucleotide sequence