Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03155 and BWI76_RS03160 are separated by 463 nucleotides BWI76_RS03160 and BWI76_RS03165 are separated by 768 nucleotides
BWI76_RS03155: BWI76_RS03155 - hypothetical protein, at 650,064 to 650,966
_RS03155
BWI76_RS03160: BWI76_RS03160 - hypothetical protein, at 651,430 to 653,463
_RS03160
BWI76_RS03165: BWI76_RS03165 - phage polarity suppression protein, at 654,232 to 654,798
_RS03165
Position (kb) 651
652
653
654 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 650.541 kb on + strand at 650.541 kb on + strand, within BWI76_RS03155 at 650.542 kb on + strand, within BWI76_RS03155 at 650.542 kb on + strand, within BWI76_RS03155 at 650.549 kb on - strand, within BWI76_RS03155 at 650.550 kb on - strand, within BWI76_RS03155 at 650.550 kb on - strand, within BWI76_RS03155 at 650.557 kb on + strand, within BWI76_RS03155 at 650.560 kb on - strand, within BWI76_RS03155 at 650.565 kb on - strand, within BWI76_RS03155 at 650.807 kb on - strand, within BWI76_RS03155 at 650.807 kb on - strand, within BWI76_RS03155 at 650.877 kb on + strand at 650.939 kb on + strand at 650.946 kb on - strand at 651.045 kb on - strand at 651.088 kb on + strand at 651.088 kb on + strand at 651.088 kb on + strand at 651.096 kb on - strand at 651.135 kb on + strand at 651.135 kb on + strand at 651.135 kb on + strand at 651.143 kb on - strand at 651.143 kb on - strand at 651.143 kb on - strand at 651.143 kb on - strand at 651.181 kb on + strand at 651.181 kb on + strand at 651.181 kb on + strand at 651.189 kb on - strand at 651.189 kb on - strand at 651.189 kb on - strand at 651.189 kb on - strand at 651.193 kb on + strand at 651.201 kb on - strand at 651.215 kb on + strand at 651.215 kb on + strand at 651.215 kb on + strand at 651.278 kb on + strand at 651.287 kb on - strand at 651.292 kb on + strand at 651.300 kb on + strand at 651.300 kb on - strand at 651.300 kb on - strand at 651.319 kb on + strand at 651.415 kb on + strand at 651.438 kb on + strand at 651.446 kb on - strand at 651.446 kb on - strand at 651.483 kb on + strand at 651.484 kb on - strand at 651.491 kb on + strand at 651.498 kb on + strand at 651.552 kb on + strand at 651.552 kb on + strand at 651.552 kb on + strand at 651.552 kb on + strand at 651.552 kb on + strand at 651.552 kb on + strand at 651.552 kb on + strand at 651.560 kb on - strand at 651.560 kb on - strand at 651.560 kb on - strand at 651.560 kb on - strand at 651.560 kb on - strand at 651.560 kb on - strand at 651.610 kb on + strand at 651.755 kb on - strand, within BWI76_RS03160 at 651.844 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.845 kb on + strand, within BWI76_RS03160 at 651.853 kb on - strand, within BWI76_RS03160 at 651.853 kb on - strand, within BWI76_RS03160 at 651.853 kb on - strand, within BWI76_RS03160 at 651.853 kb on - strand, within BWI76_RS03160 at 651.853 kb on - strand, within BWI76_RS03160 at 651.853 kb on - strand, within BWI76_RS03160 at 651.855 kb on + strand, within BWI76_RS03160 at 651.855 kb on + strand, within BWI76_RS03160 at 651.863 kb on - strand, within BWI76_RS03160 at 651.969 kb on + strand, within BWI76_RS03160 at 651.984 kb on + strand, within BWI76_RS03160 at 651.984 kb on + strand, within BWI76_RS03160 at 651.987 kb on - strand, within BWI76_RS03160 at 651.992 kb on - strand, within BWI76_RS03160 at 651.998 kb on + strand, within BWI76_RS03160 at 652.060 kb on - strand, within BWI76_RS03160 at 652.070 kb on - strand, within BWI76_RS03160 at 652.070 kb on - strand, within BWI76_RS03160 at 652.073 kb on + strand, within BWI76_RS03160 at 652.073 kb on + strand, within BWI76_RS03160 at 652.073 kb on + strand, within BWI76_RS03160 at 652.073 kb on + strand, within BWI76_RS03160 at 652.081 kb on - strand, within BWI76_RS03160 at 652.081 kb on - strand, within BWI76_RS03160 at 652.233 kb on + strand, within BWI76_RS03160 at 652.386 kb on - strand, within BWI76_RS03160 at 652.559 kb on - strand at 652.671 kb on - strand, within BWI76_RS03160 at 652.765 kb on - strand, within BWI76_RS03160 at 652.776 kb on - strand, within BWI76_RS03160 at 652.786 kb on - strand, within BWI76_RS03160 at 652.788 kb on - strand, within BWI76_RS03160 at 652.800 kb on + strand, within BWI76_RS03160 at 652.822 kb on + strand, within BWI76_RS03160 at 652.844 kb on + strand, within BWI76_RS03160 at 652.868 kb on + strand, within BWI76_RS03160 at 652.868 kb on + strand, within BWI76_RS03160 at 652.869 kb on + strand, within BWI76_RS03160 at 652.885 kb on + strand, within BWI76_RS03160 at 652.893 kb on + strand, within BWI76_RS03160 at 652.893 kb on - strand, within BWI76_RS03160 at 652.895 kb on + strand, within BWI76_RS03160 at 652.931 kb on + strand, within BWI76_RS03160 at 652.983 kb on + strand, within BWI76_RS03160 at 652.983 kb on + strand, within BWI76_RS03160 at 653.122 kb on + strand, within BWI76_RS03160 at 653.131 kb on + strand, within BWI76_RS03160 at 653.146 kb on + strand, within BWI76_RS03160 at 653.148 kb on + strand, within BWI76_RS03160 at 653.154 kb on - strand, within BWI76_RS03160 at 653.265 kb on + strand at 653.276 kb on + strand at 653.403 kb on + strand at 653.456 kb on + strand at 653.456 kb on + strand at 653.509 kb on - strand at 653.509 kb on - strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.629 kb on - strand at 653.629 kb on - strand at 653.659 kb on + strand at 653.664 kb on + strand at 653.667 kb on + strand at 653.667 kb on + strand at 653.674 kb on + strand at 653.674 kb on + strand at 653.682 kb on - strand at 653.735 kb on - strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.749 kb on - strand at 653.770 kb on + strand at 653.770 kb on + strand at 653.778 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.783 kb on + strand at 653.783 kb on + strand at 653.783 kb on + strand at 653.788 kb on + strand at 653.788 kb on + strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.791 kb on - strand at 653.791 kb on - strand at 653.792 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.796 kb on - strand at 653.796 kb on - strand at 653.798 kb on + strand at 653.801 kb on - strand at 653.801 kb on - strand at 653.808 kb on - strand at 653.871 kb on + strand at 653.878 kb on + strand at 653.878 kb on + strand at 653.878 kb on - strand at 653.881 kb on + strand at 653.889 kb on - strand at 653.889 kb on - strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.905 kb on - strand at 653.907 kb on + strand at 653.907 kb on + strand at 653.914 kb on + strand at 653.915 kb on - strand at 653.915 kb on - strand at 653.915 kb on - strand at 653.922 kb on - strand at 653.923 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.946 kb on - strand at 653.948 kb on - strand at 653.948 kb on - strand at 653.956 kb on - strand at 653.972 kb on - strand at 653.977 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 654.249 kb on - strand at 654.249 kb on - strand at 654.321 kb on + strand, within BWI76_RS03165 at 654.416 kb on - strand, within BWI76_RS03165 at 654.420 kb on + strand, within BWI76_RS03165 at 654.436 kb on + strand, within BWI76_RS03165
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 650,541 + -1.0 650,541 + BWI76_RS03155 0.53 -0.0 650,542 + BWI76_RS03155 0.53 -0.8 650,542 + BWI76_RS03155 0.53 -1.4 650,549 - BWI76_RS03155 0.54 -1.0 650,550 - BWI76_RS03155 0.54 -0.6 650,550 - BWI76_RS03155 0.54 -1.6 650,557 + BWI76_RS03155 0.55 -2.5 650,560 - BWI76_RS03155 0.55 -0.0 650,565 - BWI76_RS03155 0.55 -1.7 650,807 - BWI76_RS03155 0.82 -0.0 650,807 - BWI76_RS03155 0.82 +1.9 650,877 + +0.8 650,939 + -3.4 650,946 - -1.5 651,045 - -0.0 651,088 + +2.5 651,088 + -0.0 651,088 + +1.0 651,096 - -0.3 651,135 + -0.5 651,135 + -1.7 651,135 + +1.0 651,143 - +1.0 651,143 - -1.6 651,143 - -1.2 651,143 - -1.6 651,181 + -0.6 651,181 + -1.0 651,181 + -0.8 651,189 - -1.0 651,189 - -2.5 651,189 - +0.5 651,189 - +0.5 651,193 + -2.5 651,201 - -0.6 651,215 + -2.7 651,215 + -0.6 651,215 + -0.8 651,278 + -0.6 651,287 - -0.0 651,292 + -0.0 651,300 + +1.0 651,300 - -0.4 651,300 - -0.0 651,319 + -2.0 651,415 + -3.4 651,438 + -0.9 651,446 - -0.7 651,446 - -0.0 651,483 + -2.4 651,484 - -0.6 651,491 + +1.0 651,498 + -1.6 651,552 + -1.9 651,552 + -1.9 651,552 + +1.0 651,552 + -0.1 651,552 + -0.4 651,552 + -0.6 651,552 + +1.7 651,560 - -0.6 651,560 - +1.4 651,560 - +0.6 651,560 - -1.0 651,560 - -3.0 651,560 - -0.6 651,610 + -1.0 651,755 - BWI76_RS03160 0.16 +1.0 651,844 + BWI76_RS03160 0.20 -0.0 651,845 + BWI76_RS03160 0.20 -2.5 651,845 + BWI76_RS03160 0.20 +1.0 651,845 + BWI76_RS03160 0.20 -1.6 651,845 + BWI76_RS03160 0.20 -0.8 651,845 + BWI76_RS03160 0.20 +1.0 651,845 + BWI76_RS03160 0.20 +1.0 651,845 + BWI76_RS03160 0.20 -2.6 651,845 + BWI76_RS03160 0.20 -1.4 651,845 + BWI76_RS03160 0.20 +1.0 651,845 + BWI76_RS03160 0.20 -1.5 651,845 + BWI76_RS03160 0.20 -1.6 651,853 - BWI76_RS03160 0.21 +1.0 651,853 - BWI76_RS03160 0.21 +1.0 651,853 - BWI76_RS03160 0.21 -1.0 651,853 - BWI76_RS03160 0.21 -0.2 651,853 - BWI76_RS03160 0.21 -3.2 651,853 - BWI76_RS03160 0.21 -2.2 651,855 + BWI76_RS03160 0.21 +1.0 651,855 + BWI76_RS03160 0.21 -0.6 651,863 - BWI76_RS03160 0.21 -3.0 651,969 + BWI76_RS03160 0.26 -1.0 651,984 + BWI76_RS03160 0.27 -0.6 651,984 + BWI76_RS03160 0.27 -2.4 651,987 - BWI76_RS03160 0.27 -0.6 651,992 - BWI76_RS03160 0.28 -2.0 651,998 + BWI76_RS03160 0.28 -0.0 652,060 - BWI76_RS03160 0.31 -0.9 652,070 - BWI76_RS03160 0.31 +0.4 652,070 - BWI76_RS03160 0.31 -3.9 652,073 + BWI76_RS03160 0.32 -0.0 652,073 + BWI76_RS03160 0.32 +1.5 652,073 + BWI76_RS03160 0.32 -1.0 652,073 + BWI76_RS03160 0.32 +1.2 652,081 - BWI76_RS03160 0.32 +1.0 652,081 - BWI76_RS03160 0.32 +1.0 652,233 + BWI76_RS03160 0.39 +1.4 652,386 - BWI76_RS03160 0.47 -2.7 652,559 - +1.0 652,671 - BWI76_RS03160 0.61 +1.2 652,765 - BWI76_RS03160 0.66 +2.5 652,776 - BWI76_RS03160 0.66 -0.8 652,786 - BWI76_RS03160 0.67 -1.6 652,788 - BWI76_RS03160 0.67 -2.4 652,800 + BWI76_RS03160 0.67 +1.1 652,822 + BWI76_RS03160 0.68 -2.4 652,844 + BWI76_RS03160 0.70 +1.0 652,868 + BWI76_RS03160 0.71 +0.3 652,868 + BWI76_RS03160 0.71 -1.6 652,869 + BWI76_RS03160 0.71 -0.6 652,885 + BWI76_RS03160 0.72 -1.0 652,893 + BWI76_RS03160 0.72 -2.0 652,893 - BWI76_RS03160 0.72 -2.0 652,895 + BWI76_RS03160 0.72 -0.0 652,931 + BWI76_RS03160 0.74 +0.4 652,983 + BWI76_RS03160 0.76 -3.2 652,983 + BWI76_RS03160 0.76 -0.8 653,122 + BWI76_RS03160 0.83 +1.0 653,131 + BWI76_RS03160 0.84 +0.7 653,146 + BWI76_RS03160 0.84 -3.0 653,148 + BWI76_RS03160 0.84 -0.0 653,154 - BWI76_RS03160 0.85 +1.0 653,265 + -0.6 653,276 + -0.6 653,403 + +1.0 653,456 + -1.6 653,456 + -0.7 653,509 - -1.1 653,509 - +0.9 653,621 + +0.2 653,621 + -0.2 653,621 + -0.6 653,621 + -0.6 653,621 + +0.8 653,621 + -1.6 653,621 + -1.5 653,626 + -1.7 653,626 + -0.6 653,626 + -0.6 653,626 + -2.4 653,626 + +0.2 653,626 + -1.1 653,626 + -0.4 653,626 + +0.2 653,629 - +0.4 653,629 - -1.4 653,659 + +0.7 653,664 + -0.9 653,667 + -1.6 653,667 + -1.9 653,674 + +1.9 653,674 + +0.4 653,682 - -0.3 653,735 - -1.2 653,736 + +2.2 653,736 + -1.0 653,736 + -1.1 653,736 + +0.3 653,736 + +0.4 653,736 + -1.3 653,736 + +0.9 653,744 - +0.9 653,744 - -0.5 653,744 - +0.0 653,744 - -0.3 653,744 - -1.1 653,744 - -1.4 653,744 - +0.9 653,749 - -0.1 653,770 + -0.6 653,770 + +0.5 653,778 + -1.1 653,782 + -1.6 653,782 + -0.6 653,782 + +0.9 653,782 + +0.9 653,782 + -2.4 653,782 + +0.9 653,782 + +2.3 653,782 + -1.4 653,783 + -0.1 653,783 + -1.5 653,783 + +0.9 653,788 + +0.0 653,788 + -2.4 653,790 - -0.3 653,790 - -0.6 653,790 - -0.8 653,790 - -0.2 653,790 - -1.1 653,790 - +1.2 653,790 - +0.5 653,790 - -1.1 653,790 - -1.3 653,790 - +0.4 653,790 - -1.6 653,791 - +2.0 653,791 - -0.4 653,792 + -1.2 653,793 + -0.3 653,793 + +0.5 653,793 + +0.4 653,793 + -0.9 653,793 + +0.4 653,793 + -1.1 653,793 + +0.3 653,793 + +1.9 653,796 - +1.9 653,796 - -1.9 653,798 + -2.8 653,801 - -0.6 653,801 - -0.2 653,808 - +0.6 653,871 + -1.9 653,878 + +0.1 653,878 + -3.5 653,878 - -1.2 653,881 + -0.1 653,889 - -0.6 653,889 - -3.4 653,892 + -0.1 653,892 + +0.3 653,892 + +0.9 653,892 + -1.1 653,900 - +1.0 653,900 - -0.1 653,900 - -0.6 653,900 - +0.1 653,900 - -1.2 653,900 - -0.1 653,900 - -1.1 653,905 - -2.3 653,907 + -1.1 653,907 + +0.9 653,914 + -0.6 653,915 - -0.9 653,915 - -0.1 653,915 - -2.5 653,922 - +0.9 653,923 + -0.6 653,925 + -1.6 653,925 + -0.1 653,925 + -0.1 653,925 + -0.6 653,925 + -1.6 653,925 + -0.6 653,925 + -2.4 653,925 + -0.7 653,925 + -1.1 653,925 + +0.6 653,925 + -0.6 653,933 - +0.9 653,933 - -0.9 653,933 - -0.9 653,933 - -0.6 653,933 - -0.7 653,933 - -1.1 653,933 - -0.0 653,933 - -0.1 653,933 - -1.1 653,933 - +0.2 653,933 - +0.9 653,933 - +0.5 653,933 - +1.9 653,933 - +0.2 653,943 - +0.3 653,943 - -0.6 653,943 - +0.9 653,943 - -1.5 653,946 - -2.1 653,948 - -0.0 653,948 - +0.8 653,956 - -0.5 653,972 - +0.1 653,977 - +0.3 653,982 - -0.3 653,982 - +0.0 653,982 - -0.1 653,982 - +0.9 654,249 - -0.6 654,249 - +0.9 654,321 + BWI76_RS03165 0.16 +0.6 654,416 - BWI76_RS03165 0.32 +1.2 654,420 + BWI76_RS03165 0.33 +1.3 654,436 + BWI76_RS03165 0.36 -0.4
Or see this region's nucleotide sequence