Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01865 and BWI76_RS01870 are separated by 129 nucleotides BWI76_RS01870 and BWI76_RS01875 are separated by 122 nucleotides BWI76_RS01875 and BWI76_RS01880 are separated by 69 nucleotides
BWI76_RS01865: BWI76_RS01865 - glycerol-3-phosphate 1-O-acyltransferase, at 380,061 to 382,484
_RS01865
BWI76_RS01870: BWI76_RS01870 - diacylglycerol kinase, at 382,614 to 382,982
_RS01870
BWI76_RS01875: BWI76_RS01875 - repressor LexA, at 383,105 to 383,713
_RS01875
BWI76_RS01880: BWI76_RS01880 - MATE family efflux transporter, at 383,783 to 385,099
_RS01880
Position (kb) 382
383 Strain fitness (log2 ratio) -2
-1
0
1 at 382.488 kb on - strand at 382.536 kb on - strand at 382.571 kb on + strand at 382.571 kb on + strand at 382.571 kb on + strand at 382.627 kb on + strand at 382.764 kb on - strand, within BWI76_RS01870 at 382.798 kb on - strand, within BWI76_RS01870 at 382.803 kb on - strand, within BWI76_RS01870 at 382.873 kb on + strand, within BWI76_RS01870 at 382.907 kb on - strand, within BWI76_RS01870 at 382.995 kb on - strand at 383.810 kb on + strand at 383.868 kb on - strand at 383.899 kb on - strand at 383.927 kb on - strand, within BWI76_RS01880 at 383.952 kb on + strand, within BWI76_RS01880
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 382,488 - +0.3 382,536 - -1.0 382,571 + +0.2 382,571 + +1.0 382,571 + +0.2 382,627 + +0.7 382,764 - BWI76_RS01870 0.41 -0.2 382,798 - BWI76_RS01870 0.50 -1.6 382,803 - BWI76_RS01870 0.51 -2.2 382,873 + BWI76_RS01870 0.70 -0.9 382,907 - BWI76_RS01870 0.79 -0.6 382,995 - +1.6 383,810 + +0.2 383,868 - -0.2 383,899 - -0.3 383,927 - BWI76_RS01880 0.11 +0.8 383,952 + BWI76_RS01880 0.13 +0.9
Or see this region's nucleotide sequence