Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25265 and BWI76_RS25270 are separated by 22 nucleotides BWI76_RS25270 and BWI76_RS25275 are separated by 65 nucleotides BWI76_RS25275 and BWI76_RS25280 are separated by 45 nucleotides BWI76_RS25280 and BWI76_RS25285 overlap by 4 nucleotides BWI76_RS25285 and BWI76_RS25290 are separated by 53 nucleotides
BWI76_RS25265: BWI76_RS25265 - RNA polymerase factor sigma-54, at 5,251,847 to 5,253,280
_RS25265
BWI76_RS25270: BWI76_RS25270 - ribosome hibernation promoting factor, at 5,253,303 to 5,253,590
_RS25270
BWI76_RS25275: BWI76_RS25275 - PTS IIA-like nitrogen regulatory protein PtsN, at 5,253,656 to 5,254,144
_RS25275
BWI76_RS25280: BWI76_RS25280 - RNase adaptor protein RapZ, at 5,254,190 to 5,255,044
_RS25280
BWI76_RS25285: BWI76_RS25285 - HPr family phosphocarrier protein, at 5,255,041 to 5,255,313
_RS25285
BWI76_RS25290: BWI76_RS25290 - monofunctional biosynthetic peptidoglycan transglycosylase, at 5,255,367 to 5,256,092
_RS25290
Position (kb) 5254
5255
5256 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5253.303 kb on + strand at 5253.477 kb on + strand, within BWI76_RS25270 at 5253.483 kb on + strand, within BWI76_RS25270 at 5253.502 kb on + strand, within BWI76_RS25270 at 5254.184 kb on + strand at 5254.201 kb on - strand at 5254.555 kb on + strand, within BWI76_RS25280 at 5255.053 kb on - strand at 5255.069 kb on + strand, within BWI76_RS25285 at 5255.356 kb on - strand at 5255.396 kb on - strand at 5255.680 kb on - strand, within BWI76_RS25290 at 5255.899 kb on - strand, within BWI76_RS25290 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.994 kb on + strand, within BWI76_RS25290 at 5255.997 kb on - strand, within BWI76_RS25290 at 5256.013 kb on + strand, within BWI76_RS25290 at 5256.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 5,253,303 + +1.0 5,253,477 + BWI76_RS25270 0.60 +0.4 5,253,483 + BWI76_RS25270 0.62 -2.0 5,253,502 + BWI76_RS25270 0.69 +0.1 5,254,184 + -1.8 5,254,201 - +0.0 5,254,555 + BWI76_RS25280 0.43 -1.8 5,255,053 - +1.3 5,255,069 + BWI76_RS25285 0.10 +0.3 5,255,356 - +1.0 5,255,396 - +0.4 5,255,680 - BWI76_RS25290 0.43 -0.3 5,255,899 - BWI76_RS25290 0.73 +1.2 5,255,989 + BWI76_RS25290 0.86 -2.6 5,255,989 + BWI76_RS25290 0.86 +2.2 5,255,994 + BWI76_RS25290 0.86 +0.9 5,255,997 - BWI76_RS25290 0.87 +0.3 5,256,013 + BWI76_RS25290 0.89 -0.7 5,256,021 - +1.9
Or see this region's nucleotide sequence