Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23915 and BWI76_RS23920 are separated by 377 nucleotides BWI76_RS23920 and BWI76_RS23925 are separated by 269 nucleotides
BWI76_RS23915: BWI76_RS23915 - 5-formyltetrahydrofolate cyclo-ligase, at 4,990,550 to 4,991,146
_RS23915
BWI76_RS23920: BWI76_RS23920 - D-3-phosphoglycerate dehydrogenase, at 4,991,524 to 4,992,756
_RS23920
BWI76_RS23925: BWI76_RS23925 - ribose-5-phosphate isomerase, at 4,993,026 to 4,993,685
_RS23925
Position (kb)
4991
4992
4993 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4990.770 kb on - strand, within BWI76_RS23915 at 4991.082 kb on + strand, within BWI76_RS23915 at 4991.197 kb on + strand at 4991.198 kb on + strand at 4991.205 kb on - strand at 4991.354 kb on - strand at 4991.744 kb on + strand, within BWI76_RS23920 at 4991.783 kb on + strand, within BWI76_RS23920 at 4991.801 kb on - strand, within BWI76_RS23920 at 4991.924 kb on - strand, within BWI76_RS23920 at 4992.264 kb on - strand, within BWI76_RS23920 at 4992.658 kb on + strand at 4992.671 kb on + strand at 4992.746 kb on + strand at 4992.746 kb on + strand at 4992.802 kb on - strand at 4993.048 kb on + strand at 4993.057 kb on + strand at 4993.407 kb on + strand, within BWI76_RS23925 at 4993.628 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,990,770 - BWI76_RS23915 0.37 -0.5 4,991,082 + BWI76_RS23915 0.89 +0.5 4,991,197 + -1.7 4,991,198 + +0.1 4,991,205 - +0.0 4,991,354 - +1.6 4,991,744 + BWI76_RS23920 0.18 -1.0 4,991,783 + BWI76_RS23920 0.21 -2.2 4,991,801 - BWI76_RS23920 0.22 -3.5 4,991,924 - BWI76_RS23920 0.32 +1.0 4,992,264 - BWI76_RS23920 0.60 +0.0 4,992,658 + +0.0 4,992,671 + -2.6 4,992,746 + -1.0 4,992,746 + +1.0 4,992,802 - +0.0 4,993,048 + -0.9 4,993,057 + -1.6 4,993,407 + BWI76_RS23925 0.58 +1.0 4,993,628 + -0.6
Or see this region's nucleotide sequence