Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21740

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21735 and BWI76_RS21740 are separated by 116 nucleotidesBWI76_RS21740 and BWI76_RS21745 are separated by 185 nucleotides BWI76_RS21735: BWI76_RS21735 - aldehyde oxidase, at 4,542,789 to 4,543,754 _RS21735 BWI76_RS21740: BWI76_RS21740 - transcriptional regulator, LysR family protein, at 4,543,871 to 4,544,764 _RS21740 BWI76_RS21745: BWI76_RS21745 - LysR family transcriptional regulator, at 4,544,950 to 4,545,837 _RS21745 Position (kb) 4543 4544 4545Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4542.902 kb on + strand, within BWI76_RS21735at 4542.910 kb on - strand, within BWI76_RS21735at 4542.928 kb on + strand, within BWI76_RS21735at 4542.936 kb on - strand, within BWI76_RS21735at 4542.936 kb on - strand, within BWI76_RS21735at 4542.936 kb on - strand, within BWI76_RS21735at 4543.006 kb on + strand, within BWI76_RS21735at 4543.089 kb on + strand, within BWI76_RS21735at 4543.091 kb on - strand, within BWI76_RS21735at 4543.099 kb on + strand, within BWI76_RS21735at 4543.122 kb on - strand, within BWI76_RS21735at 4543.195 kb on + strand, within BWI76_RS21735at 4543.195 kb on + strand, within BWI76_RS21735at 4543.203 kb on - strand, within BWI76_RS21735at 4543.203 kb on - strand, within BWI76_RS21735at 4543.249 kb on + strand, within BWI76_RS21735at 4543.410 kb on - strand, within BWI76_RS21735at 4543.458 kb on + strand, within BWI76_RS21735at 4543.563 kb on - strand, within BWI76_RS21735at 4543.627 kb on - strand, within BWI76_RS21735at 4543.627 kb on - strand, within BWI76_RS21735at 4543.664 kb on - strandat 4543.691 kb on - strandat 4543.731 kb on - strandat 4543.801 kb on + strandat 4543.801 kb on + strandat 4543.809 kb on - strandat 4543.956 kb on - strandat 4544.193 kb on - strand, within BWI76_RS21740at 4544.230 kb on + strand, within BWI76_RS21740at 4544.378 kb on - strand, within BWI76_RS21740at 4544.485 kb on + strand, within BWI76_RS21740at 4544.696 kb on - strandat 4544.702 kb on - strandat 4544.755 kb on + strandat 4544.820 kb on + strandat 4544.828 kb on - strandat 4545.017 kb on + strandat 4545.025 kb on - strandat 4545.049 kb on - strand, within BWI76_RS21745at 4545.083 kb on - strand, within BWI76_RS21745at 4545.176 kb on + strand, within BWI76_RS21745at 4545.185 kb on + strand, within BWI76_RS21745at 4545.194 kb on + strand, within BWI76_RS21745at 4545.202 kb on - strand, within BWI76_RS21745at 4545.299 kb on - strand, within BWI76_RS21745at 4545.336 kb on + strandat 4545.381 kb on + strand, within BWI76_RS21745at 4545.418 kb on + strand, within BWI76_RS21745at 4545.418 kb on + strandat 4545.426 kb on - strand, within BWI76_RS21745at 4545.427 kb on + strand, within BWI76_RS21745at 4545.428 kb on + strand, within BWI76_RS21745at 4545.515 kb on - strandat 4545.646 kb on - strand, within BWI76_RS21745at 4545.660 kb on + strand, within BWI76_RS21745at 4545.668 kb on - strand, within BWI76_RS21745at 4545.668 kb on - strand, within BWI76_RS21745at 4545.673 kb on - strand, within BWI76_RS21745at 4545.673 kb on - strand, within BWI76_RS21745at 4545.702 kb on + strand, within BWI76_RS21745

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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4,542,902 + BWI76_RS21735 0.12 +1.0
4,542,910 - BWI76_RS21735 0.13 -0.5
4,542,928 + BWI76_RS21735 0.14 +1.1
4,542,936 - BWI76_RS21735 0.15 +0.2
4,542,936 - BWI76_RS21735 0.15 +0.4
4,542,936 - BWI76_RS21735 0.15 +1.2
4,543,006 + BWI76_RS21735 0.22 +0.2
4,543,089 + BWI76_RS21735 0.31 +0.4
4,543,091 - BWI76_RS21735 0.31 +0.3
4,543,099 + BWI76_RS21735 0.32 +0.1
4,543,122 - BWI76_RS21735 0.34 +0.9
4,543,195 + BWI76_RS21735 0.42 +0.3
4,543,195 + BWI76_RS21735 0.42 +0.6
4,543,203 - BWI76_RS21735 0.43 +0.1
4,543,203 - BWI76_RS21735 0.43 -0.1
4,543,249 + BWI76_RS21735 0.48 -0.0
4,543,410 - BWI76_RS21735 0.64 +1.3
4,543,458 + BWI76_RS21735 0.69 +0.4
4,543,563 - BWI76_RS21735 0.80 +1.0
4,543,627 - BWI76_RS21735 0.87 +2.0
4,543,627 - BWI76_RS21735 0.87 +0.3
4,543,664 - +0.3
4,543,691 - -0.4
4,543,731 - -0.0
4,543,801 + +0.1
4,543,801 + +0.5
4,543,809 - -0.3
4,543,956 - +0.1
4,544,193 - BWI76_RS21740 0.36 +0.4
4,544,230 + BWI76_RS21740 0.40 -0.4
4,544,378 - BWI76_RS21740 0.57 -1.7
4,544,485 + BWI76_RS21740 0.69 -1.7
4,544,696 - -0.2
4,544,702 - +0.4
4,544,755 + -0.1
4,544,820 + -0.3
4,544,828 - +0.1
4,545,017 + -0.1
4,545,025 - +0.2
4,545,049 - BWI76_RS21745 0.11 +2.8
4,545,083 - BWI76_RS21745 0.15 +1.2
4,545,176 + BWI76_RS21745 0.25 -1.6
4,545,185 + BWI76_RS21745 0.26 +0.5
4,545,194 + BWI76_RS21745 0.27 +1.2
4,545,202 - BWI76_RS21745 0.28 +0.4
4,545,299 - BWI76_RS21745 0.39 +0.9
4,545,336 + +0.2
4,545,381 + BWI76_RS21745 0.49 +0.7
4,545,418 + BWI76_RS21745 0.53 -0.2
4,545,418 + -0.6
4,545,426 - BWI76_RS21745 0.54 -0.1
4,545,427 + BWI76_RS21745 0.54 +0.0
4,545,428 + BWI76_RS21745 0.54 +0.8
4,545,515 - +2.5
4,545,646 - BWI76_RS21745 0.78 -0.3
4,545,660 + BWI76_RS21745 0.80 -0.5
4,545,668 - BWI76_RS21745 0.81 +0.3
4,545,668 - BWI76_RS21745 0.81 -1.3
4,545,673 - BWI76_RS21745 0.81 -0.1
4,545,673 - BWI76_RS21745 0.81 -2.9
4,545,702 + BWI76_RS21745 0.85 +0.1

Or see this region's nucleotide sequence