Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14065 and BWI76_RS14070 are separated by 5 nucleotides BWI76_RS14070 and BWI76_RS14075 are separated by 17 nucleotides BWI76_RS14075 and BWI76_RS14080 are separated by 11 nucleotides
BWI76_RS14065: BWI76_RS14065 - hypothetical protein, at 2,939,961 to 2,940,386
_RS14065
BWI76_RS14070: BWI76_RS14070 - ECF subfamily RNA polymerase sigma-24 subunit, at 2,940,392 to 2,940,952
_RS14070
BWI76_RS14075: BWI76_RS14075 - TetR family transcriptional regulator, at 2,940,970 to 2,941,605
_RS14075
BWI76_RS14080: BWI76_RS14080 - hydrophobe/amphiphile efflux-1 family RND transporter, at 2,941,617 to 2,944,724
_RS14080
Position (kb)
2940
2941
2942 Strain fitness (log2 ratio)
-1
0
1
2 at 2940.146 kb on - strand, within BWI76_RS14065 at 2940.253 kb on + strand, within BWI76_RS14065 at 2940.261 kb on - strand, within BWI76_RS14065 at 2940.760 kb on - strand, within BWI76_RS14070 at 2941.102 kb on + strand, within BWI76_RS14075 at 2941.246 kb on - strand, within BWI76_RS14075 at 2941.809 kb on + strand at 2942.107 kb on + strand, within BWI76_RS14080 at 2942.131 kb on + strand, within BWI76_RS14080 at 2942.308 kb on + strand, within BWI76_RS14080
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,940,146 - BWI76_RS14065 0.43 -0.2 2,940,253 + BWI76_RS14065 0.69 -0.8 2,940,261 - BWI76_RS14065 0.70 +1.8 2,940,760 - BWI76_RS14070 0.66 +0.4 2,941,102 + BWI76_RS14075 0.21 -1.5 2,941,246 - BWI76_RS14075 0.43 -1.4 2,941,809 + +0.3 2,942,107 + BWI76_RS14080 0.16 -0.0 2,942,131 + BWI76_RS14080 0.17 +0.3 2,942,308 + BWI76_RS14080 0.22 +0.1
Or see this region's nucleotide sequence