Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01695 and BWI76_RS01700 are separated by 243 nucleotides BWI76_RS01700 and BWI76_RS01705 are separated by 236 nucleotides BWI76_RS01705 and BWI76_RS01710 overlap by 1 nucleotides BWI76_RS01710 and BWI76_RS01715 are separated by 31 nucleotides
BWI76_RS01695: BWI76_RS01695 - IS110 family transposase, at 348,913 to 349,893
_RS01695
BWI76_RS01700: BWI76_RS01700 - dipeptidase E, at 350,137 to 350,826
_RS01700
BWI76_RS01705: BWI76_RS01705 - CcdB antidote CcdA, at 351,063 to 351,356
_RS01705
BWI76_RS01710: BWI76_RS01710 - DNA gyrase inhibitor, at 351,356 to 351,673
_RS01710
BWI76_RS01715: BWI76_RS01715 - shikimate 5-dehydrogenase, at 351,705 to 352,514
_RS01715
Position (kb) 350
351 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 349.492 kb on - strand, within BWI76_RS01695 at 349.569 kb on + strand, within BWI76_RS01695 at 349.607 kb on + strand, within BWI76_RS01695 at 349.633 kb on - strand, within BWI76_RS01695 at 349.633 kb on - strand, within BWI76_RS01695 at 349.680 kb on - strand, within BWI76_RS01695 at 349.680 kb on - strand, within BWI76_RS01695 at 349.708 kb on + strand, within BWI76_RS01695 at 349.733 kb on - strand, within BWI76_RS01695 at 349.743 kb on - strand, within BWI76_RS01695 at 349.845 kb on - strand at 349.860 kb on + strand at 350.096 kb on - strand at 350.153 kb on + strand at 350.188 kb on - strand at 350.202 kb on - strand at 350.219 kb on + strand, within BWI76_RS01700 at 350.219 kb on + strand, within BWI76_RS01700 at 350.282 kb on - strand, within BWI76_RS01700 at 350.293 kb on - strand, within BWI76_RS01700 at 350.293 kb on - strand, within BWI76_RS01700 at 350.457 kb on - strand, within BWI76_RS01700 at 350.457 kb on - strand, within BWI76_RS01700 at 350.722 kb on - strand, within BWI76_RS01700 at 350.780 kb on - strand at 350.908 kb on - strand at 350.909 kb on + strand at 350.922 kb on - strand at 350.922 kb on - strand at 350.963 kb on - strand at 351.034 kb on - strand at 351.258 kb on + strand, within BWI76_RS01705 at 351.310 kb on + strand, within BWI76_RS01705 at 351.443 kb on + strand, within BWI76_RS01710 at 351.458 kb on - strand, within BWI76_RS01710 at 351.622 kb on + strand, within BWI76_RS01710 at 351.622 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.635 kb on - strand, within BWI76_RS01710 at 351.682 kb on + strand at 351.682 kb on + strand at 351.690 kb on - strand at 351.722 kb on - strand at 351.777 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 349,492 - BWI76_RS01695 0.59 +0.4 349,569 + BWI76_RS01695 0.67 +0.4 349,607 + BWI76_RS01695 0.71 +0.8 349,633 - BWI76_RS01695 0.73 +0.1 349,633 - BWI76_RS01695 0.73 +0.8 349,680 - BWI76_RS01695 0.78 -1.3 349,680 - BWI76_RS01695 0.78 -1.2 349,708 + BWI76_RS01695 0.81 -3.1 349,733 - BWI76_RS01695 0.84 +0.3 349,743 - BWI76_RS01695 0.85 -0.5 349,845 - +0.7 349,860 + -1.6 350,096 - -1.2 350,153 + +1.0 350,188 - +0.7 350,202 - +1.2 350,219 + BWI76_RS01700 0.12 +0.3 350,219 + BWI76_RS01700 0.12 +0.4 350,282 - BWI76_RS01700 0.21 +0.8 350,293 - BWI76_RS01700 0.23 +0.5 350,293 - BWI76_RS01700 0.23 +1.5 350,457 - BWI76_RS01700 0.46 -3.8 350,457 - BWI76_RS01700 0.46 +0.6 350,722 - BWI76_RS01700 0.85 +0.5 350,780 - +0.2 350,908 - +0.5 350,909 + +0.2 350,922 - +0.8 350,922 - +0.5 350,963 - +0.6 351,034 - +1.1 351,258 + BWI76_RS01705 0.66 -0.9 351,310 + BWI76_RS01705 0.84 +1.1 351,443 + BWI76_RS01710 0.27 +0.3 351,458 - BWI76_RS01710 0.32 +1.1 351,622 + BWI76_RS01710 0.84 +0.5 351,622 + BWI76_RS01710 0.84 +0.8 351,627 + BWI76_RS01710 0.85 +0.5 351,627 + BWI76_RS01710 0.85 -0.2 351,627 + BWI76_RS01710 0.85 +0.9 351,630 - BWI76_RS01710 0.86 +0.2 351,630 - BWI76_RS01710 0.86 -0.5 351,630 - BWI76_RS01710 0.86 -0.8 351,635 - BWI76_RS01710 0.88 +0.6 351,682 + -1.9 351,682 + +0.3 351,690 - +0.2 351,722 - +0.7 351,777 + +1.0
Or see this region's nucleotide sequence