Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00095

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00090 and BWI76_RS00095 overlap by 32 nucleotidesBWI76_RS00095 and BWI76_RS00100 are separated by 147 nucleotidesBWI76_RS00100 and BWI76_RS00105 are separated by 37 nucleotides BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393 _RS00090 BWI76_RS00095: BWI76_RS00095 - DNA-binding transcriptional regulator, at 18,362 to 19,321 _RS00095 BWI76_RS00100: BWI76_RS00100 - hypothetical protein, at 19,469 to 20,206 _RS00100 BWI76_RS00105: BWI76_RS00105 - NAD(P)H-dependent oxidoreductase, at 20,244 to 20,810 _RS00105 Position (kb) 18 19 20Strain fitness (log2 ratio) -3 -2 -1 0 1at 17.498 kb on - strand, within BWI76_RS00090at 17.539 kb on + strand, within BWI76_RS00090at 17.546 kb on + strand, within BWI76_RS00090at 17.672 kb on + strand, within BWI76_RS00090at 17.683 kb on + strand, within BWI76_RS00090at 17.688 kb on + strand, within BWI76_RS00090at 17.696 kb on - strand, within BWI76_RS00090at 17.881 kb on - strand, within BWI76_RS00090at 17.963 kb on + strand, within BWI76_RS00090at 18.025 kb on - strand, within BWI76_RS00090at 18.069 kb on - strand, within BWI76_RS00090at 18.111 kb on + strand, within BWI76_RS00090at 18.167 kb on + strand, within BWI76_RS00090at 18.217 kb on - strand, within BWI76_RS00090at 18.219 kb on - strand, within BWI76_RS00090at 18.343 kb on - strandat 18.381 kb on + strandat 18.544 kb on - strandat 18.570 kb on + strand, within BWI76_RS00095at 18.904 kb on + strand, within BWI76_RS00095at 19.096 kb on + strand, within BWI76_RS00095at 19.227 kb on + strandat 19.300 kb on + strandat 19.308 kb on - strandat 19.308 kb on - strandat 19.313 kb on - strandat 19.623 kb on - strand, within BWI76_RS00100at 19.625 kb on - strand, within BWI76_RS00100at 19.967 kb on + strand, within BWI76_RS00100at 20.030 kb on + strand, within BWI76_RS00100at 20.038 kb on - strand, within BWI76_RS00100at 20.053 kb on - strand, within BWI76_RS00100at 20.123 kb on + strand, within BWI76_RS00100at 20.244 kb on - strandat 20.281 kb on - strandat 20.314 kb on + strand, within BWI76_RS00105

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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17,498 - BWI76_RS00090 0.24 +0.2
17,539 + BWI76_RS00090 0.28 -0.9
17,546 + BWI76_RS00090 0.28 -2.0
17,672 + BWI76_RS00090 0.39 +1.1
17,683 + BWI76_RS00090 0.40 +0.9
17,688 + BWI76_RS00090 0.40 +1.3
17,696 - BWI76_RS00090 0.41 +0.1
17,881 - BWI76_RS00090 0.57 +0.3
17,963 + BWI76_RS00090 0.64 +0.2
18,025 - BWI76_RS00090 0.69 +0.1
18,069 - BWI76_RS00090 0.73 -0.8
18,111 + BWI76_RS00090 0.76 +0.2
18,167 + BWI76_RS00090 0.81 +0.2
18,217 - BWI76_RS00090 0.85 -0.7
18,219 - BWI76_RS00090 0.85 +0.5
18,343 - +0.1
18,381 + -0.0
18,544 - -0.8
18,570 + BWI76_RS00095 0.22 -1.3
18,904 + BWI76_RS00095 0.56 -3.3
19,096 + BWI76_RS00095 0.76 -1.4
19,227 + -3.2
19,300 + -0.1
19,308 - +1.2
19,308 - +0.5
19,313 - -0.9
19,623 - BWI76_RS00100 0.21 -0.4
19,625 - BWI76_RS00100 0.21 +0.3
19,967 + BWI76_RS00100 0.67 -0.8
20,030 + BWI76_RS00100 0.76 -0.4
20,038 - BWI76_RS00100 0.77 -0.8
20,053 - BWI76_RS00100 0.79 -1.1
20,123 + BWI76_RS00100 0.89 +0.2
20,244 - +0.8
20,281 - -0.8
20,314 + BWI76_RS00105 0.12 -0.1

Or see this region's nucleotide sequence