Experiment: Betaine (C)(N); with MOPS; with chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS20840 and PFLU_RS20845 are separated by 148 nucleotides PFLU_RS20845 and PFLU_RS20850 are separated by 176 nucleotides PFLU_RS20850 and PFLU_RS20855 are separated by 14 nucleotides
PFLU_RS20840: PFLU_RS20840 - hypothetical protein, at 4,686,353 to 4,687,036
_RS20840
PFLU_RS20845: PFLU_RS20845 - hypothetical protein, at 4,687,185 to 4,687,427
_RS20845
PFLU_RS20850: PFLU_RS20850 - hypothetical protein, at 4,687,604 to 4,688,002
_RS20850
PFLU_RS20855: PFLU_RS20855 - LysE family translocator, at 4,688,017 to 4,688,625
_RS20855
Position (kb) 4687
4688 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4686.209 kb on + strand at 4686.209 kb on + strand at 4686.209 kb on + strand at 4686.210 kb on - strand at 4686.210 kb on - strand at 4686.210 kb on - strand at 4686.244 kb on - strand at 4686.354 kb on + strand at 4686.354 kb on + strand at 4686.354 kb on + strand at 4686.354 kb on + strand at 4686.354 kb on + strand at 4686.354 kb on + strand at 4686.354 kb on + strand at 4686.355 kb on - strand at 4686.408 kb on + strand at 4686.552 kb on + strand, within PFLU_RS20840 at 4686.552 kb on + strand, within PFLU_RS20840 at 4686.553 kb on - strand, within PFLU_RS20840 at 4686.553 kb on - strand, within PFLU_RS20840 at 4686.683 kb on - strand, within PFLU_RS20840 at 4686.684 kb on + strand, within PFLU_RS20840 at 4686.685 kb on - strand, within PFLU_RS20840 at 4686.685 kb on - strand, within PFLU_RS20840 at 4686.685 kb on - strand, within PFLU_RS20840 at 4686.846 kb on + strand, within PFLU_RS20840 at 4686.846 kb on + strand, within PFLU_RS20840 at 4686.847 kb on - strand, within PFLU_RS20840 at 4686.847 kb on - strand, within PFLU_RS20840 at 4686.856 kb on + strand, within PFLU_RS20840 at 4686.856 kb on + strand, within PFLU_RS20840 at 4686.857 kb on - strand, within PFLU_RS20840 at 4686.857 kb on - strand, within PFLU_RS20840 at 4686.857 kb on - strand, within PFLU_RS20840 at 4686.875 kb on + strand, within PFLU_RS20840 at 4686.876 kb on - strand, within PFLU_RS20840 at 4686.876 kb on - strand, within PFLU_RS20840 at 4686.930 kb on + strand, within PFLU_RS20840 at 4686.930 kb on + strand, within PFLU_RS20840 at 4686.930 kb on + strand, within PFLU_RS20840 at 4686.930 kb on + strand, within PFLU_RS20840 at 4686.930 kb on + strand, within PFLU_RS20840 at 4686.930 kb on + strand, within PFLU_RS20840 at 4686.930 kb on + strand, within PFLU_RS20840 at 4686.930 kb on + strand, within PFLU_RS20840 at 4686.930 kb on + strand, within PFLU_RS20840 at 4686.931 kb on - strand, within PFLU_RS20840 at 4686.931 kb on - strand, within PFLU_RS20840 at 4686.931 kb on - strand, within PFLU_RS20840 at 4686.931 kb on - strand, within PFLU_RS20840 at 4686.931 kb on - strand, within PFLU_RS20840 at 4686.936 kb on + strand, within PFLU_RS20840 at 4686.936 kb on + strand, within PFLU_RS20840 at 4686.966 kb on + strand, within PFLU_RS20840 at 4686.992 kb on - strand at 4687.000 kb on - strand at 4687.000 kb on - strand at 4687.000 kb on - strand at 4687.002 kb on + strand at 4687.003 kb on - strand at 4687.015 kb on - strand at 4687.106 kb on + strand at 4687.106 kb on + strand at 4687.106 kb on + strand at 4687.106 kb on + strand at 4687.107 kb on - strand at 4687.113 kb on + strand at 4687.143 kb on + strand at 4687.143 kb on + strand at 4687.144 kb on - strand at 4687.201 kb on + strand at 4687.202 kb on - strand at 4687.313 kb on + strand, within PFLU_RS20845 at 4687.313 kb on + strand, within PFLU_RS20845 at 4687.313 kb on + strand, within PFLU_RS20845 at 4687.314 kb on - strand, within PFLU_RS20845 at 4687.314 kb on - strand, within PFLU_RS20845 at 4687.447 kb on + strand at 4687.448 kb on - strand at 4687.817 kb on + strand, within PFLU_RS20850 at 4687.817 kb on + strand, within PFLU_RS20850 at 4687.817 kb on + strand, within PFLU_RS20850 at 4687.817 kb on + strand, within PFLU_RS20850 at 4687.818 kb on - strand, within PFLU_RS20850 at 4687.818 kb on - strand, within PFLU_RS20850 at 4687.835 kb on + strand, within PFLU_RS20850 at 4687.835 kb on + strand, within PFLU_RS20850 at 4687.835 kb on + strand, within PFLU_RS20850 at 4687.835 kb on + strand, within PFLU_RS20850 at 4687.836 kb on - strand, within PFLU_RS20850 at 4687.836 kb on - strand, within PFLU_RS20850 at 4687.836 kb on - strand, within PFLU_RS20850 at 4687.836 kb on - strand, within PFLU_RS20850 at 4687.836 kb on - strand, within PFLU_RS20850 at 4687.874 kb on + strand, within PFLU_RS20850 at 4687.875 kb on - strand, within PFLU_RS20850 at 4687.898 kb on + strand, within PFLU_RS20850 at 4687.898 kb on + strand, within PFLU_RS20850 at 4687.899 kb on - strand, within PFLU_RS20850 at 4687.900 kb on + strand, within PFLU_RS20850 at 4687.900 kb on + strand, within PFLU_RS20850 at 4687.901 kb on - strand, within PFLU_RS20850 at 4687.901 kb on - strand, within PFLU_RS20850 at 4687.901 kb on - strand, within PFLU_RS20850 at 4687.901 kb on - strand, within PFLU_RS20850 at 4687.901 kb on - strand, within PFLU_RS20850 at 4687.901 kb on - strand, within PFLU_RS20850 at 4687.901 kb on - strand, within PFLU_RS20850 at 4687.901 kb on - strand, within PFLU_RS20850 at 4688.022 kb on + strand at 4688.022 kb on + strand at 4688.022 kb on + strand at 4688.022 kb on + strand at 4688.023 kb on - strand at 4688.023 kb on - strand at 4688.023 kb on - strand at 4688.023 kb on - strand at 4688.023 kb on - strand at 4688.023 kb on - strand at 4688.023 kb on - strand at 4688.023 kb on - strand at 4688.023 kb on - strand at 4688.023 kb on - strand at 4688.073 kb on + strand at 4688.073 kb on + strand at 4688.074 kb on - strand at 4688.382 kb on - strand, within PFLU_RS20855 at 4688.382 kb on - strand, within PFLU_RS20855
Per-strain Table
Position Strand Gene LocusTag Fraction Betaine (C)(N); with MOPS; with chloride remove 4,686,209 + +0.4 4,686,209 + +0.1 4,686,209 + -0.5 4,686,210 - -2.3 4,686,210 - +0.0 4,686,210 - +0.0 4,686,244 - -2.7 4,686,354 + +1.1 4,686,354 + +0.3 4,686,354 + -0.2 4,686,354 + +1.2 4,686,354 + +0.6 4,686,354 + +0.2 4,686,354 + +1.0 4,686,355 - +0.8 4,686,408 + +0.8 4,686,552 + PFLU_RS20840 0.29 +0.4 4,686,552 + PFLU_RS20840 0.29 +1.1 4,686,553 - PFLU_RS20840 0.29 +0.5 4,686,553 - PFLU_RS20840 0.29 +0.7 4,686,683 - PFLU_RS20840 0.48 +0.3 4,686,684 + PFLU_RS20840 0.48 -1.3 4,686,685 - PFLU_RS20840 0.49 +0.6 4,686,685 - PFLU_RS20840 0.49 -2.0 4,686,685 - PFLU_RS20840 0.49 +0.3 4,686,846 + PFLU_RS20840 0.72 +0.3 4,686,846 + PFLU_RS20840 0.72 +2.6 4,686,847 - PFLU_RS20840 0.72 -0.3 4,686,847 - PFLU_RS20840 0.72 -0.5 4,686,856 + PFLU_RS20840 0.74 +0.3 4,686,856 + PFLU_RS20840 0.74 +0.4 4,686,857 - PFLU_RS20840 0.74 -1.0 4,686,857 - PFLU_RS20840 0.74 -0.7 4,686,857 - PFLU_RS20840 0.74 +0.3 4,686,875 + PFLU_RS20840 0.76 +0.4 4,686,876 - PFLU_RS20840 0.76 +0.4 4,686,876 - PFLU_RS20840 0.76 +0.1 4,686,930 + PFLU_RS20840 0.84 -0.2 4,686,930 + PFLU_RS20840 0.84 +0.5 4,686,930 + PFLU_RS20840 0.84 -0.5 4,686,930 + PFLU_RS20840 0.84 +0.6 4,686,930 + PFLU_RS20840 0.84 -0.9 4,686,930 + PFLU_RS20840 0.84 +0.9 4,686,930 + PFLU_RS20840 0.84 -1.7 4,686,930 + PFLU_RS20840 0.84 -0.3 4,686,930 + PFLU_RS20840 0.84 -0.2 4,686,931 - PFLU_RS20840 0.85 +2.5 4,686,931 - PFLU_RS20840 0.85 +2.1 4,686,931 - PFLU_RS20840 0.85 +1.8 4,686,931 - PFLU_RS20840 0.85 +0.8 4,686,931 - PFLU_RS20840 0.85 +1.2 4,686,936 + PFLU_RS20840 0.85 +0.3 4,686,936 + PFLU_RS20840 0.85 +0.4 4,686,966 + PFLU_RS20840 0.90 -0.7 4,686,992 - -0.0 4,687,000 - -0.0 4,687,000 - +2.1 4,687,000 - +1.2 4,687,002 + -1.3 4,687,003 - -0.2 4,687,015 - +0.7 4,687,106 + -3.1 4,687,106 + +0.8 4,687,106 + +0.8 4,687,106 + +1.4 4,687,107 - -0.2 4,687,113 + +0.0 4,687,143 + -1.5 4,687,143 + +1.0 4,687,144 - -1.0 4,687,201 + -1.0 4,687,202 - +2.0 4,687,313 + PFLU_RS20845 0.53 -1.0 4,687,313 + PFLU_RS20845 0.53 -0.7 4,687,313 + PFLU_RS20845 0.53 +0.5 4,687,314 - PFLU_RS20845 0.53 -1.3 4,687,314 - PFLU_RS20845 0.53 -2.2 4,687,447 + +1.3 4,687,448 - -0.1 4,687,817 + PFLU_RS20850 0.53 -1.5 4,687,817 + PFLU_RS20850 0.53 +0.3 4,687,817 + PFLU_RS20850 0.53 +0.5 4,687,817 + PFLU_RS20850 0.53 -0.5 4,687,818 - PFLU_RS20850 0.54 -0.5 4,687,818 - PFLU_RS20850 0.54 +3.5 4,687,835 + PFLU_RS20850 0.58 +0.1 4,687,835 + PFLU_RS20850 0.58 -0.7 4,687,835 + PFLU_RS20850 0.58 -0.7 4,687,835 + PFLU_RS20850 0.58 +0.6 4,687,836 - PFLU_RS20850 0.58 +0.4 4,687,836 - PFLU_RS20850 0.58 +0.6 4,687,836 - PFLU_RS20850 0.58 -0.7 4,687,836 - PFLU_RS20850 0.58 -2.0 4,687,836 - PFLU_RS20850 0.58 -0.7 4,687,874 + PFLU_RS20850 0.68 +2.1 4,687,875 - PFLU_RS20850 0.68 -1.7 4,687,898 + PFLU_RS20850 0.74 +1.4 4,687,898 + PFLU_RS20850 0.74 +0.8 4,687,899 - PFLU_RS20850 0.74 -1.3 4,687,900 + PFLU_RS20850 0.74 +0.4 4,687,900 + PFLU_RS20850 0.74 -1.0 4,687,901 - PFLU_RS20850 0.74 +0.1 4,687,901 - PFLU_RS20850 0.74 +3.2 4,687,901 - PFLU_RS20850 0.74 -1.2 4,687,901 - PFLU_RS20850 0.74 -0.7 4,687,901 - PFLU_RS20850 0.74 +0.4 4,687,901 - PFLU_RS20850 0.74 -0.5 4,687,901 - PFLU_RS20850 0.74 -2.0 4,687,901 - PFLU_RS20850 0.74 -1.2 4,688,022 + -0.5 4,688,022 + +0.3 4,688,022 + -1.3 4,688,022 + -2.2 4,688,023 - -0.2 4,688,023 - +0.5 4,688,023 - -0.2 4,688,023 - -1.6 4,688,023 - -0.7 4,688,023 - -0.6 4,688,023 - +2.2 4,688,023 - -2.2 4,688,023 - -0.2 4,688,023 - -1.1 4,688,073 + -1.6 4,688,073 + -1.6 4,688,074 - -1.5 4,688,382 - PFLU_RS20855 0.60 -0.8 4,688,382 - PFLU_RS20855 0.60 +0.8
Or see this region's nucleotide sequence