Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS14725

Experiment: Betaine (C)(N); with MOPS; with chloride

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Betaine (C)(N); with MOPS; with chloride
3,289,287	-		PFLU_RS14715	0.57	+6.3
3,289,287	-		PFLU_RS14715	0.57	+5.3
3,289,287	-		PFLU_RS14715	0.57	+6.1
3,289,299	-		PFLU_RS14715	0.59	+6.6
3,289,299	-		PFLU_RS14715	0.59	+5.7
3,289,299	-		PFLU_RS14715	0.59	+5.9
3,289,299	-		PFLU_RS14715	0.59	+6.7
3,289,335	-		PFLU_RS14715	0.63	+6.5
3,289,335	-		PFLU_RS14715	0.63	+5.8
3,289,335	-		PFLU_RS14715	0.63	+6.1
3,289,338	-		PFLU_RS14715	0.64	+5.9
3,289,338	-		PFLU_RS14715	0.64	+6.2
3,289,338	-		PFLU_RS14715	0.64	+5.8
3,289,338	-		PFLU_RS14715	0.64	+6.2
3,289,338	-		PFLU_RS14715	0.64	+5.2
3,289,338	-		PFLU_RS14715	0.64	+6.0
3,289,338	-		PFLU_RS14715	0.64	+5.6
3,289,338	-		PFLU_RS14715	0.64	+6.2
3,289,338	-		PFLU_RS14715	0.64	+5.7
3,289,470	-		PFLU_RS14715	0.80	+3.9
3,289,629	+				+0.5
3,289,630	-				+3.9
3,289,630	-				+3.4
3,289,630	-				+4.6
3,289,630	-				+4.0
3,289,630	-				+3.4
3,289,630	-				-0.0
3,289,669	-				+1.3
3,289,670	+				-1.1
3,289,670	+				+0.2
3,289,671	-				-0.3
3,289,671	-				+1.3
3,289,671	-				-0.3
3,289,671	-				-1.5
3,289,671	-				-1.0
3,289,707	-		PFLU_RS14720	0.13	-1.0
3,289,722	-		PFLU_RS14720	0.15	+0.3
3,289,722	-		PFLU_RS14720	0.15	-1.9
3,289,722	-		PFLU_RS14720	0.15	-0.6
3,289,722	-		PFLU_RS14720	0.15	-0.3
3,289,724	+		PFLU_RS14720	0.16	-1.9
3,289,724	+		PFLU_RS14720	0.16	+0.7
3,289,724	+		PFLU_RS14720	0.16	+2.0
3,289,724	+		PFLU_RS14720	0.16	+0.0
3,289,724	+		PFLU_RS14720	0.16	-2.3
3,289,724	+		PFLU_RS14720	0.16	+2.3
3,289,725	-		PFLU_RS14720	0.16	-1.0
3,289,725	-		PFLU_RS14720	0.16	+0.3
3,289,725	-		PFLU_RS14720	0.16	+0.1
3,289,725	-		PFLU_RS14720	0.16	+0.3
3,289,725	-		PFLU_RS14720	0.16	-0.7
3,289,725	-		PFLU_RS14720	0.16	+0.5
3,289,725	-		PFLU_RS14720	0.16	-0.7
3,289,725	-		PFLU_RS14720	0.16	-0.7
3,289,725	-		PFLU_RS14720	0.16	-1.7
3,289,758	+		PFLU_RS14720	0.21	+2.2
3,289,794	+		PFLU_RS14720	0.27	-1.0
3,289,795	-		PFLU_RS14720	0.27	-0.1
3,289,802	+		PFLU_RS14720	0.28	+0.6
3,289,882	+		PFLU_RS14720	0.42	-2.3
3,289,882	+		PFLU_RS14720	0.42	-0.4
3,289,882	+		PFLU_RS14720	0.42	+0.2
3,289,882	+		PFLU_RS14720	0.42	+2.0
3,289,882	+		PFLU_RS14720	0.42	+0.3
3,289,883	-		PFLU_RS14720	0.42	-2.6
3,289,883	-		PFLU_RS14720	0.42	-1.7
3,289,883	-		PFLU_RS14720	0.42	-1.6
3,290,009	-		PFLU_RS14720	0.63	-0.6
3,290,009	-		PFLU_RS14720	0.63	-3.0
3,290,074	+		PFLU_RS14720	0.73	+2.3
3,290,074	+		PFLU_RS14720	0.73	+1.9
3,290,075	-		PFLU_RS14720	0.73	-0.7
3,290,221	+				-1.7
3,290,221	+				-2.5
3,290,222	-				-2.4
3,290,233	+				-1.6
3,290,233	+				-2.5
3,290,233	+				-1.9
3,290,233	+				+0.7
3,290,233	+				+0.1
3,290,233	+				+0.1
3,290,233	+				-0.7
3,290,233	+				-1.9
3,290,233	+				-2.2
3,290,233	+				-1.7
3,290,233	+				+0.9
3,290,233	+				-0.7
3,290,233	+				-1.2
3,290,233	+				+0.3
3,290,233	+				+1.3
3,290,233	+				+0.1
3,290,233	+				+0.1
3,290,233	+				-0.3
3,290,233	+				-1.1
3,290,233	+				+1.3
3,290,233	+				+0.3
3,290,233	+				-3.6
3,290,234	-				+0.3
3,290,234	-				+2.5
3,290,234	-				-0.0
3,290,234	-				-1.4
3,290,234	-				-2.0
3,290,234	-				-1.7
3,290,234	-				-1.4
3,290,234	-				+0.3
3,290,234	-				-0.7
3,290,234	-				-1.0
3,290,234	-				-1.7
3,290,234	-				-0.1
3,290,234	-				-1.3
3,290,234	-				-0.3
3,290,234	-				+0.9
3,290,234	-				+0.3
3,290,234	-				-2.0
3,290,237	+				-1.9
3,290,237	+				+0.3
3,290,237	+				-0.5
3,290,237	+				-0.3
3,290,237	+				-2.1
3,290,237	+				-0.3
3,290,237	+				-0.8
3,290,237	+				-1.0
3,290,237	+				+0.5
3,290,238	-				-0.7
3,290,238	-				-0.9
3,290,238	-				+1.3
3,290,238	-				-1.4
3,290,238	-				-2.1
3,290,238	-				-1.0
3,290,238	-				+0.3
3,290,238	-				+1.3
3,290,238	-				-0.7
3,290,511	+		PFLU_RS14725	0.24	-2.0
3,290,512	-		PFLU_RS14725	0.24	-2.5
3,290,512	-		PFLU_RS14725	0.24	-0.3
3,290,526	+		PFLU_RS14725	0.25	-1.4
3,290,610	+		PFLU_RS14725	0.32	-0.3
3,290,725	+		PFLU_RS14725	0.42	-1.5
3,290,725	+		PFLU_RS14725	0.42	-0.3
3,290,725	+		PFLU_RS14725	0.42	-2.0
3,290,725	+		PFLU_RS14725	0.42	-0.3
3,290,725	+		PFLU_RS14725	0.42	+1.3
3,290,726	-		PFLU_RS14725	0.42	-1.3
3,290,729	+		PFLU_RS14725	0.42	-1.7
3,290,729	+		PFLU_RS14725	0.42	-1.9
3,290,729	+		PFLU_RS14725	0.42	-0.2
3,290,729	+		PFLU_RS14725	0.42	-1.9
3,290,729	+		PFLU_RS14725	0.42	-1.9
3,290,730	-		PFLU_RS14725	0.42	-3.4
3,290,730	-		PFLU_RS14725	0.42	-1.5
3,290,730	-		PFLU_RS14725	0.42	-1.3
3,290,730	-		PFLU_RS14725	0.42	-0.7
3,290,730	-		PFLU_RS14725	0.42	+0.3
3,290,771	+		PFLU_RS14725	0.45	-1.0
3,290,772	-		PFLU_RS14725	0.45	-0.7
3,290,868	-		PFLU_RS14725	0.54	-1.9
3,290,868	-		PFLU_RS14725	0.54	-1.3
3,291,080	+		PFLU_RS14725	0.71	-1.0
3,291,080	+		PFLU_RS14725	0.71	-1.0
3,291,080	+		PFLU_RS14725	0.71	-0.0
3,291,080	+		PFLU_RS14725	0.71	-1.9
3,291,081	-		PFLU_RS14725	0.71	-0.9
3,291,081	-		PFLU_RS14725	0.71	-1.9
3,291,081	-		PFLU_RS14725	0.71	-1.9
3,291,081	-		PFLU_RS14725	0.71	-0.7
3,291,174	+		PFLU_RS14725	0.79	-0.5
3,291,175	-		PFLU_RS14725	0.79	-2.0
3,291,175	-		PFLU_RS14725	0.79	-0.3
3,291,175	-		PFLU_RS14725	0.79	-2.8
3,291,314	+				-2.2
3,291,315	-				-2.0
3,291,315	-				-1.3
3,291,371	-				-2.2
3,291,371	-				-1.0
3,291,469	+				-0.2
3,291,469	+				-0.2
3,291,470	-				-1.5
3,291,470	-				-1.3
3,291,470	-				-0.3
3,291,470	-				-0.1
3,291,555	+		PFLU_RS14730	0.14	+1.2
3,291,555	+		PFLU_RS14730	0.14	+0.3
3,291,555	+		PFLU_RS14730	0.14	-0.8
3,291,556	-		PFLU_RS14730	0.14	-0.7
3,291,556	-		PFLU_RS14730	0.14	-2.0
3,291,556	-		PFLU_RS14730	0.14	+0.1
3,291,574	+		PFLU_RS14730	0.19	-0.2
3,291,574	+		PFLU_RS14730	0.19	-0.3
3,291,574	+		PFLU_RS14730	0.19	-1.2
3,291,575	-		PFLU_RS14730	0.19	-0.0
3,291,575	-		PFLU_RS14730	0.19	-0.3
3,291,575	-		PFLU_RS14730	0.19	+1.3
3,291,727	-		PFLU_RS14730	0.60	+0.7
3,291,727	-		PFLU_RS14730	0.60	+1.1
3,291,731	+		PFLU_RS14730	0.61	-0.7
3,291,732	-		PFLU_RS14730	0.61	-1.6
3,291,789	+		PFLU_RS14730	0.76	+0.7
3,291,789	+		PFLU_RS14730	0.76	-0.4
3,291,790	-		PFLU_RS14730	0.76	-0.7
3,291,790	-		PFLU_RS14730	0.76	-0.9
3,291,790	-		PFLU_RS14730	0.76	-0.5
3,291,790	-		PFLU_RS14730	0.76	-0.3
3,291,790	-		PFLU_RS14730	0.76	-1.0
3,291,790	-		PFLU_RS14730	0.76	+1.3
3,291,858	+				+0.8
3,291,859	-				-2.3
3,291,859	-				-1.7
3,291,877	+				+0.5
3,291,877	+				-1.5
3,291,878	-				-2.6
3,291,878	-				-2.4
3,291,878	-				-1.3
3,291,878	-				-1.8
3,291,878	-				+0.1
3,291,984	+				+1.7
3,291,985	-				-0.5
3,291,985	-				-0.7
3,291,985	-				-1.5
3,291,985	-				+1.3
3,291,997	+				+0.1
3,291,997	+				+1.4
3,291,998	-				-2.0
3,292,008	+				+1.3
3,292,009	-				+0.3
3,292,009	-				+2.6
3,292,093	+				+1.3
3,292,093	+				-0.2
3,292,094	-				-0.5
3,292,114	+				-1.3
3,292,114	+				-1.9
3,292,114	+				-0.3
3,292,114	+				+0.9
3,292,114	+				+0.3
3,292,114	+				-1.9
3,292,115	-				+1.1
3,292,115	-				-1.0
3,292,115	-				+0.3
3,292,115	-				-0.3
3,292,115	-				-0.2
3,292,115	-				-1.0
3,292,115	-				+1.3
3,292,115	-				-0.7
3,292,116	+				-0.0
3,292,116	+				-1.3
3,292,116	+				-0.0
3,292,116	+				+1.0
3,292,116	+				+0.4
3,292,116	+				+1.3
3,292,116	+				+0.3
3,292,117	-				-1.3
3,292,117	-				-0.1
3,292,117	-				+1.8
3,292,117	-				+0.1
3,292,212	+				-1.0
3,292,213	-				+1.3
3,292,242	-				+1.6
3,292,303	+				+0.9
3,292,304	-				-1.5
3,292,322	+				+1.7
3,292,322	+				-0.0
3,292,322	+				-0.7
3,292,322	+				-1.7
3,292,322	+				+0.9
3,292,322	+				-1.0
3,292,323	-				+0.1
3,292,323	-				+0.1
3,292,323	-				-2.0
3,292,323	-				-3.5
3,292,323	-				-1.3
3,292,323	-				-0.3
3,292,323	-				-0.7
3,292,335	+				+2.3
3,292,335	+				-3.1
3,292,336	-				-0.4
3,292,402	+				-0.6
3,292,402	+				-1.3
3,292,402	+				-1.3
3,292,402	+				-1.9
3,292,402	+				+1.3
3,292,402	+				-1.0
3,292,402	+				+1.3
3,292,403	-				+1.3
3,292,403	-				-0.1
3,292,403	-				+1.9
3,292,403	-				-2.6
3,292,403	-				+0.3
3,292,406	+				+1.5
3,292,406	+				+1.3
3,292,407	-				+1.5
3,292,418	-		PFLU_RS14735	0.10	-1.7